LOCUS       sequence-202906-        3620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Bleymehl K, Perez-Gomez A, Omura M, Moreno-Perez A, Macias D, Bai Z,
            Johnson RS, Leinders-Zufall T, Zufall F, Mombaerts P
  TITLE     A Sensor for Low Environmental Oxygen in the Mouse Main Olfactory 
            Epithelium.
  JOURNAL   Neuron. 2016 Dec 21;92(6):1196-1203. doi: 
            10.1016/j.neuron.2016.11.001. Epub 2016 Dec 1.
  PUBMED    27916458
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.11.001"; journalName: "Neuron"; date: 
            "2016-12-21- 21"; volume: "92"; issue: "6"; pages: "1196-1203"
COMMENT     Sequence Label: pISH-Gucy1b2-B
FEATURES             Location/Qualifiers
     source          1..3620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(785..803)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(786..803)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(821..837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(834..856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    845..861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(869..875,876..893,894..899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    914..935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1052..1069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1223..1811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1303..1322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1982..2773,2774..2842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2605..2624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2843..2947)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3025..3480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3162..3183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3374..3393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3606..3620,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3620,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tgagaattcg agacgtgtac
      121 catccttttc agcgatgttg tgacattcac caacatctgt gcggcctgtg aacctatcca
      181 aatagtgaac atgctcaact caatgtactc caagtttgac aggttaacca acatccatga
      241 tgtctataaa gtagaaacaa taggtgatgc ttacatggtg gtaggtggag taccagtacc
      301 cgttgaaagc catgctcaaa gagttgctaa ttttgctctg gggatgagaa tttctgcaaa
      361 agaagtgatg aatcctgtca ctggggaacc catacagatc agagtgggaa tccatactgg
      421 accagtctta gcaggtgttg tgggagacaa gatgccccgg tactgcctgt ttggtgacac
      481 cgtaaacacg gcctctagga tggaaagtca cgggcttccc aacaaagtgc atctgagccc
      541 cacagcccac agggctctgg aaaacaaagg gtttgaaatt gtcacgagag gggagataga
      601 ggtgaagggg aaaggaaaaa tgaccacata cttcctgatc cggaacctga atgccaccga
      661 ggttgagatc atggggcgac cttcagctct cgctgatggg aaggaatcac tagtgaattc
      721 gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
      781 gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2941 ttccgcgcac atttccccga aaagtgccac ctgatgcggt gtgaaatacc gcacagatgc
     3001 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     3061 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3121 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3181 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3241 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3301 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     3361 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     3421 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3481 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3541 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3601 gttttcccag tcacgacgtt
//
