LOCUS       sequence-202916-        4015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Pde2a
FEATURES             Location/Qualifiers
     source          1..4015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1180..1198)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1181..1198)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1216..1232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1216..1232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1229..1251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1240..1256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1264..1270,1271..1288,1289..1294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1309..1330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1447..1464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1618..2206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1698..1717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2377..3168,3169..3237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3000..3019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3238..3342)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3420..3875)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3557..3578)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3769..3788
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4001..4015,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4015,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat ttgtgacgct gcttaagaga
      121 tggagcggct tgggcaggac tgggcatgga tagtcagagg tgcagaagag acccatacca
      181 ctgcccctgg tcactactgt gctcttgtgt acaaatggca aagtctgtgt gggactggta
      241 atcttgcttg ccttctgcag atgagaaaga tgaagaagcc caggaagggt ggcatgtgcc
      301 gggtcacagg ggacatcctg gcctagaacc caggacccat ggctctggtt cccaatgcat
      361 caaacagact ggtagagagt tgtgaaagga gggccaggca gggagggtcc ctagagggga
      421 tccctcagcc ctcgaggctg cagcagccat tgacaggagc tctggtgccg tccaaatcgg
      481 ggacctcgta ttcctcgtcc aggaaatcca gcgagttgtt actggggagg cctcggatgg
      541 tgaacttgtg ggacaccttg gtccagtgct cacggttgga ggccacacgt tcatacagct
      601 ctgccgcctt ggggaacagg tcttgtaaaa gcttgtagat aggcatggca atgtgctcca
      661 taaagctgat ctgaagctca gggatgtagg ccttctcacg gtccatcatc tccatcggtc
      721 ggttgcccat ggccttctcc aagtctccct gggagaagaa ctctttgtag atcagctctg
      781 caatctttct ggtggtcttc cagccctttg tctggtcaga gaggtcacag gaggtcatga
      841 ggaggcacag aagaagcctg tggtgttgcc tgttgtttcg gtcataaccc acttcagcca
      901 tcttctgcag gtccttgaag atgcggaggt ggtgtgccag gtccgtagcc aagatgatgt
      961 ccctcatcag gtccagcatg cgctgatagt ccttccgaga gaagtggtca aagatattgc
     1021 agccgtgggt gttgaggata gcaatggctt gagcaaagtg gtgcctctcc atgacagagc
     1081 cctctgagct gtagagtgca atcactagtg aattcgcggc cgcctgcagg tcgaccatat
     1141 gggagagctc ccaacgcgtt ggatgcatag cttgagtatt ctatagtgtc acctaaatag
     1201 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1261 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     1321 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1381 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     1441 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     1501 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1561 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1621 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     1681 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1741 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1801 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1861 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1921 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1981 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2041 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2101 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2161 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2221 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2281 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2341 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2401 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     2461 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     2521 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     2581 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     2641 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     2701 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2761 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2821 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2881 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2941 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3001 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3061 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3121 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3181 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3241 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3301 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3361 gccacctgat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     3421 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     3481 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     3541 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     3601 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     3661 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     3721 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3781 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3841 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     3901 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3961 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgtt
//
