LOCUS       sequence-202917-        3779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Pde4a
FEATURES             Location/Qualifiers
     source          1..3779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      321..330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(944..962)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(945..962)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(980..996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(980..996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(993..1015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1004..1020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1028..1034,1035..1052,1053..1058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1073..1094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1211..1228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1382..1970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1462..1481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2141..2932,2933..3001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2764..2783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3002..3106)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3184..3639)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3321..3342)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3533..3552
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3765..3779,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3779,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat ttctcaggag ggccaggagt
      121 cgttgaggtg gtctgtggtg agtggagagt gtggggagga gggaggagcc gagggtgaag
      181 ggaaaaggga tcaggcaggg tctccacctg acccccacct gcctggagca gagatgacgg
      241 cagaattgtc tcctgatgtc cccgagcagg cagaacaagc cctcatggca gccagatggt
      301 ctcttggggc ctccacctct gccgccatgg aggggaggcc caggaggccc tgggcctcct
      361 ctgaggggga cagggtcctc aagtcaggca gaatagaggt ttcaaggctg aaggaatggc
      421 ccacagcctc cgcggactcc tcaggagcgc catgtgctga ggctgtctcc tgcttcaaac
      481 tctctgctac ctccacaaca gcagggctct ggcctttgac ctttgactgt tccatggctt
      541 gagctctggt atcatgtgga gccaggagag tttcctcagt gcaaggcaat cctggaacct
      601 ccaatgaatc ctcttcatcc tcttcctcca aggtgagctc aaattggaac ttgtcaggca
      661 gggccggatc gctgagcact cctggctcct cttccagggt tggggaggga ctctggcgga
      721 tggcactgtg ataccaatcc cggttgtctt ccagcgtgtc caggatgtct tgggcatcgg
      781 ggtggacgag atcggcccac gtctcccaca gtgggtgaac aatatagtcg atgaagccca
      841 cctgagactt ctccacagag gctaatcact agtgaattcg cggccgcctg caggtcgacc
      901 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      961 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1021 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1081 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1141 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1801 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3121 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3181 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3241 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3301 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3361 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3421 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3481 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3541 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3601 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     3661 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3721 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgtt
//
