LOCUS       sequence-202918-        3773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: pISH-Pde6d
FEATURES             Location/Qualifiers
     source          1..3773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(938..956)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(939..956)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(974..990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(974..990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(987..1009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    998..1014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1022..1028,1029..1046,1047..1052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1067..1088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1205..1222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1376..1964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1456..1475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2135..2926,2927..2995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2758..2777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2996..3100)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3178..3633)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3315..3336)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3527..3546
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3759..3773,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat taggaggcag gcaggtgaca
      121 ggcgcagcgt ggctcctggg agggtttgtt cccatgtttg cgccaggaag gagtcgaggc
      181 ttatccctgc ctttggctgg ggttgggagt ttagtcttgt cagtgaagat ggaggttttg
      241 ggagaaaagc cactggccgt ctattcagag ctggtttcct ggtgcacagg tgggttctgt
      301 gttgaaggag tgagtcagct ggagtatccc agtcaggtgt gcagagaaca agttcagctt
      361 cctcctgccc ccggccaaat tcttagaagt gcacacaaat tctctcaaac atagaaaagc
      421 ctcactttgg atgtgctgac aagaagatca tcgtcaaaga actttgtctc tatgatgaca
      481 ttgcccgtta ggacgctggc gggcatcatc tgggactcag gcgctgcttc tatcaaggac
      541 tgccaggtgt ttgtggagtt agggatcaca aagccaaact cgaagaacca ctcttctagg
      601 cattgtcctt tgaagtaaac tttttgttcc aggcggaatt tttccatttg ttctgctgaa
      661 gaaaagttca attctcgaga cactgccttg cacttgagga ttttcttggg cacacgggct
      721 tcatgttcca caccagggac agacaggtct tctgttcctt gccagagtat cttccctgtt
      781 tcggcatccc gaaggttcat ccaatttagt ttgaagcctc tcaggatatc cctggcccgc
      841 tcgtccttgg ctgacataat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg
      901 gagagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct
      961 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1021 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1081 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1141 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1201 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1261 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1321 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1381 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1441 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1501 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1561 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1621 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1681 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1741 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1801 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1861 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1921 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1981 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2041 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2101 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2161 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2221 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2281 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2341 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2401 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2461 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2521 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2581 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2641 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2701 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2761 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     2821 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     2881 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     2941 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3001 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3061 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3121 cacctgatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
     3181 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3241 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3301 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3361 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3421 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3481 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3541 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3601 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
     3661 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     3721 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gtt
//
