LOCUS       sequence-202923-        4997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transgenesis vector based on the Minos transposon, carrying the 
            3xP3-DsRed marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Pavlopoulos A, Averof M
  TITLE     Establishing genetic transformation for comparative developmental 
            studies in the crustacean Parhyale hawaiensis.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 May 31;102(22):7888-93. Epub 2005 May
            23.
  PUBMED    15911760
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-05-31"; volume: "102(22)"; pages: 
            "7888-93. Epub 2005 May 23"
COMMENT     Sequence Label: pMi(3xP3-DsRed)
FEATURES             Location/Qualifiers
     source          1..4997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     470..486
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     471..487
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      772..781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             778..1455
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(908..926)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(958..978)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1176..1195
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1370..1393
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    1576..1697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1613..1632)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1667..1686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1734..1750)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1734..1750)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2152..2171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2153..2171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2181..2198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2181..2197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2190..2212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2339..2794
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2426..2445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2636..2657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2820..2924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2925..2993,2994..3785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3143..3162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3956..4544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4445..4464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4698..4715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4832..4853
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4868..4873,4874..4891,4892..4898)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4906..4922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4911..4933
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4930..4946
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4930..4946
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4965..4985
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4967..4985
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccagtg cttgaatgct aaatgttttc aatttttcgc cattaagaca agcctacaca
       61 aatgcttcta taaattatgc caagcacgtt agcagcttct acgagcccca accactatta
      121 attcgaacag catgtttttt tgcagtgcgc aatgtttaac acactatatt atcaatacta
      181 ctaaagataa cacataccaa tgcatttcgt ctcaaagaga attttattct cttcacgacg
      241 aaaaaaaaag ttttgctcta tttccaacaa caacaaaaat atgagtaatt tattcaaacg
      301 gtttgcttaa gagataagaa aaaagtgacc actattaatt cgaacgcggc gtaagcttac
      361 cttaatctca agaagagcaa aacaaaagca actaatgtaa cggaatcatt atctagttat
      421 gatctgcaaa taatgctgca gagctggggg atccccggcg ggccccccct cgaggtcgac
      481 ggtatcgata agcttgatat cgaattcgag ctcgcccggg gatctaattc aattagagac
      541 taattcaatt agagctaatt caattaggat ccaagcttat cgatttcgaa ccctcgaccg
      601 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
      661 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      721 atcggggtac cgctagagtc gacggtaccg cgggcccggg atccaccggt cgccaccatg
      781 gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc
      841 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      901 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      961 tccccccagt tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac
     1021 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1081 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggctcctt catctacaag
     1141 gtgaagttca tcggcgtgaa cttcccctcc gacggccccg taatgcagaa gaagactatg
     1201 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1261 cacaaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac
     1321 atggccaaga agcccgtgca gctgcccggc tactactacg tggactccaa gctggacatc
     1381 acctcccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga gggccgccac
     1441 cacctgttcc tgtagcggcc gcgactctag atcataatca gccataccac atttgtagag
     1501 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     1561 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     1621 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1681 ctcatcaatg tatcttaaag cttatcgata cgcgtacggc gcgcctaggg ggggatccac
     1741 tagttctaga gcggccgcca ccgcgggacg ttacacaatt ctaatattaa ttaaattatt
     1801 gttttaagta tgatagtaaa tcacattacg ccgcgttcga attaatagtg gtcacttttt
     1861 tcttatctct taagcaaacc gtttgaataa attactcata tttttgttgt tgttggaaat
     1921 agagcaaaac tttttttttc gtcgtgaaga gaataaaatt ctctttgaga cgaaatgcat
     1981 tggtatgtgt tatctttagt agtattgata atatagtgtg ttaaacattg cgcactgcaa
     2041 aaaaaacatg ctgttcgaat taatagtggt tggggctcgt agaaaacgaa aaatatctta
     2101 agctagcata gagaatggag caaaactcaa tttgatgcga gctccaattc gccctatagt
     2161 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2221 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2281 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     2341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     2401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     2461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     2521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     2581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     2641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     2701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     2761 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     2821 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2881 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2941 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3001 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3061 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3121 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     3181 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3241 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3301 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     3361 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     3421 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     3481 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3541 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3601 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3661 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3721 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3781 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3841 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4081 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4621 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4681 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4741 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4801 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     4861 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     4921 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     4981 aaagggaaca aaagctg
//
