LOCUS       sequence-202928-        3996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Trpc2-probe1
FEATURES             Location/Qualifiers
     source          1..3996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1161..1179)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1162..1179)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1197..1213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1197..1213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1210..1232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1221..1237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1245..1251,1252..1269,1270..1275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1290..1311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1428..1445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1599..2187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1679..1698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2358..3149,3150..3218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2981..3000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3219..3323)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3401..3856)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3538..3559)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3750..3769
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3982..3996,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3996,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat taggcagtca ggatgatcat
      121 gaggataaac atgaaccgga tcatgtcgtc aatcatcttg ccgatggaga tctgcagtgt
      181 gcccagcgat tcgtgagctg gcagaatata tgccagtcgg gtgaagctga gcatgctggt
      241 gacagcaaag agcacctcag ccagaaactg ggggtcctct gtacgccact cacttctctc
      301 agctatggtg aaatagcggc aggtggtgct gtctgaggca tcacggcagt gcatgtaggc
      361 aagcccagcc aggaggaggc gcagtgcaaa ggatgccaag tacagggaca ggatgaccac
      421 gtccaggaag ttccaccagt ccaggaggta gctccgcaag ccctcgatcc acacctcctt
      481 gcattcaaac cataggaagc ctgtgaccca gatcatatgt agtgaagtct cccagacact
      541 ctggctgcgg cctttgaagg tgctcagctg ggtctccatg accagagact ctcccagcaa
      601 gaagataagg aaccacaggt aggaggcaga atgcagcagg aacttcagca ccgggatctt
      661 cagcaggcgg cccagctggg acttgggcgc cagccagtag ccaatgcaga ggaagggcat
      721 ggtgaggaag atgaggaagg caacaaagag cttccagatg gtggtgcttc cacgccagcc
      781 agccaggttc ccacaccaga tggaagacag aacttgctgg cagatgggat gtgctacaaa
      841 ctgtttctgg ttgtagttga cagccaaccg cagtcttgcc aggttgggga tgccctcctc
      901 aaaggcctga cccaggccct cagcctcagg ctcagtctca ctatcctcac ccaggtcatt
      961 gagcactgcg gtgacctcac tctgatttcg gcacatgccc agcaactcga agccatagtc
     1021 ctggcagaga gactccaggg caatgtactg aggcttaaac tcaggctcct ttcgtgcaag
     1081 aatcactagt gaattcgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt
     1141 tggatgcata gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca
     1201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga
     3361 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     3421 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3481 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3541 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3601 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3661 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3721 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3781 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3841 ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     3901 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     3961 gttgggtaac gccagggttt tcccagtcac gacgtt
//
