LOCUS       sequence-202931-        3939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Trpc2-probe2
FEATURES             Location/Qualifiers
     source          1..3939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1104..1122)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1105..1122)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1140..1156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1140..1156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1153..1175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1164..1180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1188..1194,1195..1212,1213..1218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1233..1254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1371..1388)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1542..2130)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1622..1641)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2301..3092,3093..3161))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2924..2943
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3162..3266)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3344..3799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3481..3502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3693..3712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3925..3939,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3939,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggccgcggga ttgcaccatg gtctttactg tgagaccctg
      121 gctctaccca acacagcagc tgcacttagt gacagaagag acccttctgt tgccttagga
      181 ctcgcccttg gtctccagat ctccttccag gagcaagtcc cctgacccct ctgcttcttg
      241 ctccctgtga accagcacat gggcagagga ggaagagccg ggctctccag gagcgggagc
      301 tccagcttca cctgtgccat caaggccatc tcctaaagag acttcggtct ccacaatccc
      361 aggcatagtc agctctgctg gggtgtcaga ggtagggggg cccaggttct ggaagctgtt
      421 gcgcactcgg gtgatgtatc tactgaggat gctggcacca tccggaggcc gtggtgcccc
      481 tcccgccgcc aggttcttct gcacactggc cacctcgctt tgcagtcgag acacagtctt
      541 ggtcagctct gtcagtctgt tgcccaggtc tttccgacgg gtctcctcga actcgcgccg
      601 ggcagtctct atgtagcgct gcaccagagc cttgatgact cgaaggcggt aggacacgcg
      661 ttctccttcc ccggcggagc ccgcccttgc cccggggttg gtaaaggtcc cgatgggcgg
      721 gtagtccgac ttcttggctt tgcagcagga ggagccacag caaaggaacc ggaaaattct
      781 cctgacgagg tagaaggcgg cctttgggga tggcaggatg ttaaagggca caggcagcgt
      841 cagaccctct cggaagtagg acaggtagag cttggagcga gcaaacttcc actccacatc
      901 agcatcatcc tcgatcttct ggaaggagtt ggtgatcatg gcaataagca tgttaagtag
      961 cacaatgacc atgacgatgg taaagatgcc gtgcatggcc ctgcccacga actcaggcac
     1021 caggaactga atcactagtg cggccgcctg caggtcgacc atatgggaga gctcccaacg
     1081 cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
     1141 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1201 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1261 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1321 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1381 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1441 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1501 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1561 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1621 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1681 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1741 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1801 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1861 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1921 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1981 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2041 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2101 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2161 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2221 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2281 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2341 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2401 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2461 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2521 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2581 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2641 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2701 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2761 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2821 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2881 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2941 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3001 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3061 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3121 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3181 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3241 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg
     3301 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     3361 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3421 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3481 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3541 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3601 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3661 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3721 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3781 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3841 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3901 taagttgggt aacgccaggg ttttcccagt cacgacgtt
//
