LOCUS       sequence-202933-        8952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rosa26 targeting vector for intersectional Cre and FLP responsive 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Lusk SJ, McKinney A, Hunt PJ, Fahey PG, Patel J, Chang A, Sun JJ, 
            Martinez VK, Zhu PJ, Egbert JR, Allen G, Jiang X, Arenkiel BR, 
            Tolias AS, Costa-Mattioli M, Ray RS
  TITLE     A CRISPR toolbox for generating intersectional genetic mouse models 
            for functional, molecular, and anatomical circuit mapping.
  JOURNAL   BMC Biol. 2022 Jan 28;20(1):28. doi: 10.1186/s12915-022-01227-0.
  PUBMED    35086530
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-01-28"; volume: "20(1):28. doi"; pages: " 
            10.1186/s12915-022-01227-0"
COMMENT     Sequence Label: 
            p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
FEATURES             Location/Qualifiers
     source          1..8952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    54..87
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             91..894
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(154..173)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     764..783
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          1437..1502
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1632..1652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2077..2211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2253..2286
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    2293..2326
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          2866..2931
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3061..3081
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3506..3640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3682..3715
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3731..4319
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3784..3804)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4202..4213)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4335..4559
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4571..5381
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    4895..5544
                     /label=HA-R
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     CDS             complement(5823..6638)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5823..6638)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6546..6565
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6630..6649
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(7099..7116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7233..7778)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7313..7332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gatgaagttc
       61 ctattctcta gaaagtatag gaacttcacc atgggatcgg ccattgaaca agatggattg
      121 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      181 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      241 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
      301 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
      361 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
      421 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
      481 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
      541 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
      601 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
      661 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
      721 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
      781 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
      841 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctaatagtga
      901 gatattaagg gttccggatc ctcggggaca ccaaatatgg cgatctcggc cttttcgttt
      961 cttggagctg ggacatgttt gccatcgatc catctaccac cagaacggcc gttagatctg
     1021 ctgccaccgt tgtttccacc gaagaaacca ccgttgccgt aaccaccacg acggttgttg
     1081 ctaaagaagc tgccaccgcc acggccaccg ttgtagccgc cgttgttgtt attgtagttg
     1141 ctactgttat ttctggcact tcttggtttt cctcttaagt gaggaggaac ataaccattc
     1201 tcgttgttgt cgttgatgct taaattttgc acttgttcgc tcagttcagc cataatatga
     1261 aatgcttttc ttgttgttct tacggaatac cacttgccac ctatcaccac aactaacttt
     1321 ttcccgttcc tccatctctt ttatattttt tttctcgagg gatctttgtg aaggaacctt
     1381 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     1441 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     1501 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     1561 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     1621 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
     1681 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     1741 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa
     1801 cctttataag taggcataac agttataatc ataacatact gttttttctt actccacaca
     1861 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
     1921 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
     1981 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2041 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2101 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2161 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgacatggt
     2221 aagtaagctt gggctgcagg tcgagggacc tagaagttcc tattctctag aaagtatagg
     2281 aacttcatca ggataacttc gtataatgta tgctatacga agttatgata tcattaaggg
     2341 ttccggatcc tcggggacac caaatatggc gatctcggcc ttttcgtttc ttggagctgg
     2401 gacatgtttg ccatcgatcc atctaccacc agaacggccg ttagatctgc tgccaccgtt
     2461 gtttccaccg aagaaaccac cgttgccgta accaccacga cggttgttgc taaagaagct
     2521 gccaccgcca cggccaccgt tgtagccgcc gttgttgtta ttgtagttgc tactgttatt
     2581 tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct cgttgttgtc
     2641 gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa atgcttttct
     2701 tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt tcccgttcct
     2761 ccatctcttt tatatttttt ttctcgaggg atctttgtga aggaacctta cttctgtggt
     2821 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     2881 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     2941 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     3001 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     3061 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     3121 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     3181 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     3241 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     3301 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     3361 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     3421 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3481 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3541 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3601 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgacatggta agtaagcttg
     3661 ggctgcaggt cgagggacct aataacttcg tataatgtat gctatacgaa gttatcctgt
     3721 ttaaacattt aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3781 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3841 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3901 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3961 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4021 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4081 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4141 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4201 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4261 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4321 tgaaataatt ccgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4381 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4441 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4501 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggt
     4561 taattaaaga agatgggcgg gagtcttctg ggcaggctta aaggctaacc tggtgtgtgg
     4621 gcgttgtcct gcaggggaat tgaacaggtg taaaattgga gggacaagac ttcccacaga
     4681 ttttcggttt tgtcgggaag ttttttaata ggggcaaata aggaaaatgg gaggataggt
     4741 agtcatctgg ggttttatgc agcaaaacta caggttatta ttgcttgtga tccgcctcgg
     4801 agtattttcc atcgaggtag attaaagaca tgctcacccg agttttatac tctcctgctt
     4861 gagatcctta ctacagtatg aaattacagt gtcgcgagtt agactatgta agcagaattt
     4921 taatcatttt taaagagccc agtacttcat atccatttct cccgctcctt ctgcagcctt
     4981 atcaaaaggt attttagaac actcatttta gccccatttt catttattat actggcttat
     5041 ccaaccccta gacagagcat tggcattttc cctttcctga tcttagaagt ctgatgactc
     5101 atgaaaccag acagattagt tacatacacc acaaatcgag gctgtagctg gggcctcaac
     5161 actgcagttc ttttataact ccttagtaca ctttttgttg atcctttgcc ttgatcctta
     5221 attttcagtg tctatcacct ctcccgtcag gtggtgttcc acatttgggc ctattctcag
     5281 tccagggagt tttacaacaa tagatgtatt gagaatccaa cctaaagctt aactttccac
     5341 tcccatgaat gcctctctcc tttttctcca tttataaact gagctattaa ccattaatgg
     5401 tttccaggtg gatgtctcct cccccaatat tacctgatgt atcttacata ttgccaggct
     5461 gatattttaa gacattaaaa ggtatatttc attattgagc cacatggtat tgattactgc
     5521 ttactaaaat tttgtcattg tacacatctg taaaaggtgg ttccttttgg aatgcaaagt
     5581 tcaggtgttt gttgtctttc ctgacctaag gtcttgtgag cttgtatttt ttctatttaa
     5641 gcagtgcttt ctcttggact ggcttgactc atggcattct acacgttatt gctggtctaa
     5701 atgtgatttt gccaagcttc ttcaggacct ataattttgc ttgacttgta gccaaacaca
     5761 agtaaaatga ttaagcaaca aatgtatttg tgaagcttgg tccaccacgc tcggagggcc
     5821 tgttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     5881 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     5941 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     6001 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     6061 actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg ttcaacaggc
     6121 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     6181 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     6241 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     6301 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
     6361 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     6421 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     6481 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
     6541 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     6601 ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
     6661 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     6721 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     6781 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     6841 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     6901 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     6961 cctcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     7021 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     7081 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     7141 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     7201 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     7261 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     7321 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     7381 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     7441 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     7501 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
     7561 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
     7621 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
     7681 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt
     7741 cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt attaatcaga
     7801 taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     7861 ccatacgata taagttgtat ttcgtcgacg gaagagcggc gcgccaaatc tccgttctgt
     7921 gagacagccg ggtacgagtc gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc
     7981 ggtccgccct gcagcaaccg gagggggagg gagaagggag cggaaaagtc tccaccggac
     8041 gcggccatgg ctcggggggg ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg
     8101 ctgattggct tcttttcctc ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc
     8161 gtaaggcgcc tgtcagttaa cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc
     8221 ccactgggtg gggcgggagg taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg
     8281 cctctggcgg ggcgggggag gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc
     8341 cgcccaccct ccccttcctc tgggggagtc gttttacccg ccgccggccg ggcctcgtcg
     8401 tctgattggc tctcggggcc cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag
     8461 ttgagtccat ccgccggcca gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc
     8521 cctcggcccc gcgccgcaga gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg
     8581 cgctgggggc ggggacgggc agtagggctg agcggctgcg gggcgggtgc aagcacgttt
     8641 ccgacttgag ttgcctcaag aggggcgtgc tgagccagac ctccatcgcg cactccgggg
     8701 agtggaggga aggagcgagg gctcagttgg gctgttttgg aggcaggaag cacttgctct
     8761 cccaaagtcg ctctgagttg ttatcagtaa gggagctgca gtggagtagg cggggagaag
     8821 gccgcaccct tctccggagg ggggagggga gtgttgcaat acctttctgg gagttctctg
     8881 ctgcctcctg gcttctgagg accgccctgg gcctgggaga atcccttccc cctcttcttt
     8941 ctttaattaa gc
//
