LOCUS       sequence-202957-        4106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Gnas
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1271..1289)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1272..1289)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1307..1323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1307..1323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1320..1342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1331..1347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1355..1361,1362..1379,1380..1385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1400..1421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1538..1555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1709..2297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1789..1808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2468..3259,3260..3328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3091..3110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3329..3433)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3511..3966)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3648..3669)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3860..3879
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4092..4106,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4106,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggccgcggga ttaagcaagg tgtgaaggga agaggggttt
      121 ttcagaatca ctgggggaaa aaggaaaggt tgcggtgttg atcatgccct atggtgggtg
      181 accaactgct tgtacaatta cgtttcactc ttaattaatt gtgcttaagg ctgaattaaa
      241 tttgggtgtt cccttcttag agcagctcgt attggcggag atgcatgcgc tggatgatgt
      301 cacggcagtc gttgaagaca cggcggatgt tctcagtgtc cacggcgcag gtaaagtgag
      361 ggtagcagta gtggcgccca tctccactag cagtgctgat tctcagaaac tcatcccgaa
      421 tgaagtactt ggcccgggtc acgcgtgggt cctctcccgg ctcgggagtc gcatcctcag
      481 gagtggtgta gcgagcgaac tctgaaaagt agtcctcaat cttcgatttg ccagcgagga
      541 ctttctcagc aagcaggtct tgcttgttga ggaagagaat cacagagatg gtgcgcagcc
      601 atctgttgtt ccagatgctc ttgaagaggt tcagagcctc ctgcaggcgg ttagtctggt
      661 tgtcctcccg aatgaccatg ttgtagctgc tgctggccac cacgaagatg atggcagtca
      721 catcattgaa gcactggatc cacttgcggc gctcatcgcg ctggccgccc acatcgaaca
      781 tgtggaagtt gactttgtcc acctggaact tggtctcaaa gattccagag gtcaggacac
      841 ggcagcgaag caggtcctgg tcacttggca cgtagtcggc ctgcttgatc acatcaatct
      901 tgtccaggaa gtactgggca cagtcaatca gctggtactc attggagcgc tcgtagcagg
      961 cacgcactcc ctcatcctcc cacagagcct tggcatgctc atagaattca ggtgggaagt
     1021 caaagttcgg cacgttcatc acgctcagaa tgtagtccac tctgaactgg ttctcagggt
     1081 tggccagctc cacagggggc accaggttgc tcatggcggc cacaatggtt tcaatggcct
     1141 ccttcaggtt gtttttgatg tcctgcactt tagtggcctt ctcatctccg ttaaacaatc
     1201 actagtgcgg ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata
     1261 gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc
     1321 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1441 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1501 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1561 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3421 taggggttcc gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac
     3481 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     3541 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3601 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3661 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3721 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3781 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3841 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3901 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3961 gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4021 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4081 gccagggttt tcccagtcac gacgtt
//
