LOCUS       sequence-202961-        3959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr54
FEATURES             Location/Qualifiers
     source          1..3959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1124..1142)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1125..1142)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1160..1176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1160..1176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1173..1195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1184..1200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1208..1214,1215..1232,1233..1238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1253..1274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1391..1408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1562..2150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1642..1661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2321..3112,3113..3181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2944..2963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3182..3286)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3364..3819)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3501..3522)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3713..3732
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3945..3959,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3959,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tctaagagga cttagagccc
      121 ctgttgagga gccttgtcac tgactccttc atgtccttgt tcctcaggct gtaaatgaag
      181 gggttcagca tgggggtaac cacagtatac atgatggtgg ctaccgtgtc ctgggtggag
      241 aaggagtctg gaggtcgtat atagataccc agtactgtgc catagaacag agtgaccaca
      301 gacaagtgtg agctacatgt ggaaaatgtc ttgtgccggc cctttgcaga ggggatctgg
      361 aggaccacca tggtgattcg gacataagag accacaatga gaatgcaggg ggccacaaag
      421 aggaccacag ctcccaggaa gacaagttga ttgactcgtg tgtctgaaca ggccaacttc
      481 atgagcaggt aagcatcaca gtagaagtga gagatctccg ggatggagga gcagaaactc
      541 agtctgctca tgaggagtgt gtgcatcaga gagatgaggg cagagcacat ccacacaacg
      601 gctgccagcc ccacacagag ctggggggtg atgatggtgt ggtactgtag tgggtagcat
      661 atggccacaa atctgtccag ggccatggca gccagcagaa aggtgtccac attggcaaaa
      721 gaaatcagga agtacgtctg agttaggcag tggacataag agatggtcct tgttccagcc
      781 acatggccag ccaggagcct ggggatgaga ttggtggtga agcagacatc aacgaaggaa
      841 aggttggcca ggaagaagta catgggggtg tgcaggcggg gatcggagat gatggccagg
      901 aagatgagtg tgttcccagc taccgtgacc aggtacatgc acagaaaagc ccagaagagg
      961 atttgttgct gctgtgggtc attggtgatg ccaagcagga gaaactcgct gactccagtc
     1021 tcattctccc tccccttgga cataatcact agtgaattcg cggccgcctg caggtcgacc
     1081 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
     1141 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1201 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1261 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1321 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2461 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3301 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     3361 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3421 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3481 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3541 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3601 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3661 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3721 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3781 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     3841 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3901 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgtt
//
