LOCUS       sequence-202966-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr78
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1131..1149)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1132..1149)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1167..1183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1167..1183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1180..1202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1191..1207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1215..1221,1222..1239,1240..1245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1260..1281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1398..1415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1569..2157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1649..1668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2328..3119,3120..3188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2951..2970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3189..3293)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3371..3826)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3508..3529)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3720..3739
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3952..3966,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3966,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tcagcttcaa tgtccttgtc
      121 acagctgatc ttgaacatag ccagaacccg tgttctgatc tgtttggtct tagcaccgta
      181 gatgatggga ttgatcacag gaggcagcag caggtagaca tcccccatta gaacatgcac
      241 aatgggatcc aggctgtttc caaaacggtg caccactgac agaccaatga gtggtacata
      301 gaaagccagg accacactaa tgtgtgacac acaagtccca aatgccttag ctcgctcaga
      361 cttggaaggc agttgcagaa ccgttcgtat gatcaggaag taggacaagg agatgaacat
      421 gacatctaca cccatgacta gcagaatggc agttagacca tagactacat tgggtaatgt
      481 gtctgtatag gccaacttca tcacatcctg gtggacacaa taggagtggg agagcacatt
      541 ggagtgacag aaagccagtc gcttgatcag tagtgggagt gggaaaaaga atagggatcc
      601 ccggaccaga gccaccatgc ctatttggac tgttactgta ttgttgagga cagcagcgtg
      661 acgcagtggg tggcagatag ccacgtaccg gtcaaaggcc atggccagca ggatagtaga
      721 ttcaattgct gagagagtat gaatgaaaaa catctgggca agacaggcat caaaagtaat
      781 ctcccgggag tcaaaccaaa agagggcgag aatcttgggc attgtggatg tggacaaagc
      841 cagatcaata gctgccagca tgcagagaaa aaggtacatg ggtgcatgca ggctccgctc
      901 tgttctcact atgaagacca caatgcagtt tccaaacaat gctacagcat acatggaaag
      961 cagggggaag ccaaaccaaa agtgagcttc ctccagtcct ggaataccaa taagcaggaa
     1021 ggtggcatgg gtgaagttgc aggaactcat aatcactagt gaattcgcgg ccgcctgcag
     1081 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
     1141 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3301 ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     3361 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     3421 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     3481 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     3541 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     3601 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     3661 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     3721 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     3781 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     3841 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     3901 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     3961 gacgtt
//
