LOCUS       sequence-202972-        3975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In situ hybridization and RNAi studies in planarians.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, 
            Sanchez Alvarado A
  TITLE     Hands-On Classroom Activities for Exploring Regeneration and Stem 
            Cell Biology with Planarians
  JOURNAL   The American Biology Teacher 79(3):208-223. Mar 2017.
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "The 
            American Biology Teacher 79(3):208-223. Mar 2017."
COMMENT     Sequence Label: Gsp_piwi-1
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
ORIGIN
        1 gcaaagatgg tagagtcgtc aatttaaaat ccaactatgc caactttaac attaaaattg
       61 atggtccttt ttatatgtat gatgttgtta tggagccaaa aagtattcgg tccaaactgc
      121 aattattgat gacaaaaagt gatgggcagc cattttgttt tgatggtcgt agattattta
      181 ctacttctaa atggcattca acggatgatg tagagaaatt tactgttaac aacgtcgaaa
      241 tttcaataag gttgctgaca acatttgtaa aaagttctga agaatattat caaatgatta
      301 atattgtgtt taataatatt caaattttta tgggacaaga gaaaattggg cgacaatttt
      361 ttttgggttc gaattgtgag agtggtggaa taaaagaggg tccgagtttt ttcaaaatgg
      421 agaattttaa aattttgggc ggttattcaa ctaccattac caggggtact aatgaagatg
      481 gaacagcttc aactttatta tacttagaaa gaataaatcg tgttcttaat gaaaattcgg
      541 taatcgctgt ttatagaaga gatcaaatcg atcaattaat agggcgagac atcattacca
      601 aatacaataa taaaacatac agaatatccg atattaaaga aatgggtatt aatgacgaaa
      661 ttgaattgaa tggcactaaa atttcgtatt acaattactt taaacagcgt tataatatta
      721 ctttgaaaaa cgacaagcag ccatttgtta tttctagaat caaaagatca atgactcatc
      781 ggcctaagga aaaaagtgaa aatgaaccag aaggtccaaa ggaaacggat caaagtttat
      841 ccattccagg tgaattgtgt tatttatgcg gattttctga tagtgaaaga agcaatataa
      901 atctacaaaa aagtttaggt ggtgtgctaa aaagagagcc tcgtgaaaga ttaaacgata
      961 taaaagattt ttgcaaatgg acaggtgggg gcaaatccaa agattatatg aaaacctgga
     1021 atatgactat tgaggaacag cctattacga tacgaggtcg tgaattacct ccggtagata
     1081 ttatgtccaa cgatgttaaa attaatgcta aaactccaga tgattggaaa tttggtgaag
     1141 taaaatttga tgttcctaaa ggtataacgc atagatttgg cgttttagtt gtagatcgta
     1201 atcctagtca ttttaataat tttatcgatg atgtaaaaag agaaattagt aggctcagaa
     1261 ttaattacac tattgataat atagcttctt gtaggggtaa tgaagttgaa gacgctctta
     1321 ctgaatttgt tcgtgttaac aaagttcatt tggcgttagt atttatacct gatgataagg
     1381 tatatgcaaa agtcaaaaac tttactatgt ctactggatt gttgacacaa tgtgtgactc
     1441 aaagaaatgg aagtaaccga gatgatagac gccgtaagac cgtggctgat aaatctgtta
     1501 tgcagatatt tgctaaatta ggatacgatc cgtggggtat cagtttaaag atggctccaa
     1561 caatgattgt aggaatagat actttccata gtaaaactgg taagagatcg gtacaagcat
     1621 ccgtgttttc tataagttct aagttttctc agtatatgag ttttgtcaat tcccctaagg
     1681 gtaaaaatga atttcatgag aacttgggca aaaatttctt aacggcaatt actgcattcc
     1741 aacaaaaatt tggcactatg cctcaacgat ttattatcta tcgtgatggg gtaggagatt
     1801 cccaattatt cctcacgaaa aaattcgaga cagatgctgt aatgaaaatg gtcgaaaaga
     1861 tttatgagaa tcaaaatttg cctcaaataa tttatgtagt tgttaaaaaa cggattagtg
     1921 taaaattctt taaagatgga gctaatccga atccgggaac agtggttgat gaaaagattg
     1981 ttaaacctaa cttttatgaa ttctatttgg tttcacagcg aactactaaa ggaacagcat
     2041 ctccaacgaa ttataatgtt cttatggata cgaagtattg taataagaaa accaacgaag
     2101 tgtccccaat ggctccaagc gttttgcaac aaataactta ctctcttact cacttgtatt
     2161 ttaattggat gggtacaata agagttcctg tacccgttca ttatgcagat cgtttggcag
     2221 agttggtcgg aatagtacat cgtggaactg ctcccccaac cattaatgag agaattaggg
     2281 aacgcttatt ttatttatag aatagctgtc tatgtcaatt tttcccactt atgttattat
     2341 tggtgtctaa taaaaaaatt atttttgtag cgtgttataa ttactttttt tgaattaatg
     2401 caagttaaca aaaataaaga tcattgttgt aattcatttt atttaaatga tatagtacat
     2461 tttacaattg aaccttttta tttatctgaa gtgtaaattt ggtttcttcg ttttctttta
     2521 aaacaacccc atagattgca aaagaatttt ttctctaaat atatattgtt tggttggtat
     2581 ctgttcaaat ttttccaatt tgttggtatt tcattaattt cttcagtttg ttcaaatgac
     2641 cacgaaaatg caatgagaaa acatgttatc aatataaaac aattcaaacg catcatttta
     2701 tttgacgatc gaaaaaaaaa aaaaaggaat cagtgccaga tctgctgcaa ttgaaatttg
     2761 tgaagttgaa ggagacaaat ttgtcagtaa aaccacagct ataaaatggt ttaaaagatt
     2821 caattttagc aatacgggta ttaatgatga ttcacgttct ggaagaccca ggacagttga
     2881 tacccaagca attatgcttt ctgtggatga aaatccttgt accagtgttc gaagactttc
     2941 agacgaagtt caaattccaa aatcttcagt acaccgcaaa cgtacagctc atggcaagag
     3001 aaataggaaa tgcaaagttt ttcctcatga actgactccc gaacaagctg aaaaacgttt
     3061 gctaatctgt gaacaactcc ttcgctatcc aaatgatgaa cgatttctga aaaccatcgt
     3121 tacttgcgat gagaaatggc tgtacttccg aaatccagac ttgcggaatc agtggttaga
     3181 ccctaaccag agtcccatac cggttaccaa gtgccaacga tttgaaaaca aggttttgtt
     3241 gtgtgtttgg tggaattacg aggggcctgt gcactgggaa cttcttccgg atggtcgaac
     3301 tatcgatggt aatatttatg ctgaacaact acagcgagtg catgatgctt tgagcactcg
     3361 ttatccagct atgttgaatc gtggtaaaac actcctacag catgataatg ctccaactca
     3421 tacatcacga atagtgaagc ggaaaattca ggaactgcaa ggcatcgaag tcatacccag
     3481 cgtatagccc ggatcttgct ccttctgatt accatatgtt tctctctctg tcacatttct
     3541 tacgagggag aagctttggt tcagagaaag aggtcaaaaa tggaatcatc aaaaaaacct
     3601 gagtggtatc gccatggaat tgagcaattg tcagaaagat ggcttcgagt taaagaaaat
     3661 gatgaactat atttagagga ttaatatgat ctgtgttaaa aaaattttgt aaataaaaca
     3721 tttgcataaa aacatgaata acatttgact caatctaata tgtgaggttg taaatttgta
     3781 ttgaaatatt attacagata ttcgaagatg taaaagttgt atcttaattt tataaggtta
     3841 taaatgtatt ttattttaaa tctattatta tcttaagctg gtgttctgat ttgatctatc
     3901 aatttaaatg taatagatgt aatactttag agaaatttgt cgtcatctat acattggagt
     3961 tcaagagcat aatcg
//
