LOCUS       sequence-203023-        7175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-DN-Cep152-mKate2
FEATURES             Location/Qualifiers
     source          1..7175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..22
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3354..4052
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    4179..4300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4216..4235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4270..4289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4307..4762)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4444..4465)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4656..4675
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4789..4893
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4895..5252
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4895..5086
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4918..4938)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5103..5238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5287..6081
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5287..6081
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5341..5360)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5951..5970
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6269..6288)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6313..6360
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      join(6689..7175,1..102)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
       61 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      181 cctgattctg tggataaccg tattaccgcc atgcattagt tattactagc gctaccggac
      241 tcagatctcg agctcaagct tcgaattctg cagcccgggg gatccttcag gggtgtcgct
      301 tggttatttc caaaaatcaa attaatttta ttaaactatt agaacgagca tgttttgtct
      361 atatgctaca gaagataaaa aataatagga gttaacagtt ataaaacaac acactttgtt
      421 tctattgatt gttgaccaca ctggggtctc attaagttag attaaagaca cactaactgg
      481 gtcaaaagca gcagattgat ttcatagcac cagggtaaac tttctaacac ttttacggca
      541 atcatataca ttaaaattaa atacagacca cgactgaaca aggaggatga tctccaatat
      601 taaacaaaga gacttgtgcc tatttctctg agggtaaaca tgacctctca agttagcaag
      661 ttgtttttaa cactacaaaa atagttaaga ctgcaatccc agaataaagt attggtttta
      721 accaatcaat atagtacagt aaacatccat ttgttttgtt gaaacgttaa acaaatctga
      781 ccaaagctat tagcttatat aaaacaggtt tgccttctat gtagctgaaa acaccacagg
      841 cccgattttg ctactgtgta aaacatttca gcaagatttt tttattgcat ttttttttac
      901 tgaatcgttc aaacatttta tcattttagt ttgttcattc attgcaactg gaaaaacaac
      961 acatcacaca accgcacata tttcagcaat aagtacaata aaacactcaa ataaaaaaaa
     1021 cattttaaat ctctttgtat ttttgaccgc tgtttcgcgt aatttcacgg taaaactctg
     1081 gaaatctcca ctacattcct ctcagcggct cctctcaatg acagctgaag aagtgacgcg
     1141 gctgcctgct gtgttttgat tggtcgaatt cactggaggc ttccagaaca gtgtagagtc
     1201 tgaacgggtg cgcgctctgc tgtatttaaa gggcgaaaga gagaccgcag agaaactcaa
     1261 ccgaagagaa gcgacttgac aaagaagaaa agagcagcct gacaggactt tttccccgac
     1321 gaggtgttta ttcgctctat ttaagaatct actgtaaggt aagtctcaat atattgtact
     1381 ctattggcta atcagaatta tatagagatt atatgtactt aatgtcaaaa aatcaacttt
     1441 gtatatgtaa tctttttaca tgtggactgc ctatgttcat cttattttag gtctactaga
     1501 aaattatatt tcccgttttc acaataagga tttttaaaaa aagcaatgaa cagacgggca
     1561 tttactttat gttgctgaca ttattttata tgagcataat aaccataaat actagcaaat
     1621 gtcctaaatg aatttgtgtt aatgttgtct acaaaagaaa attagcgttt tacttgtaca
     1681 actaataata acttggttat taagagaatt tcacttgttg actagaaaaa tcctttcata
     1741 atgaaacaat tgcaccataa attgtataaa tataaaatta attctaattg tttttttttc
     1801 ctgcagtcat gtcattagac tttggcagtg tggcactacc agtgcaaaat gaagatgaag
     1861 agtatgacga agaggactat gaaagagaga aagagttgca gcagttactc acagaccttc
     1921 cccatgacat gctggatgac gacctctcct ctccagagct ccagtattcg gactgcagcg
     1981 aggatggcac agacggacaa ccacatcatc ctgagcaatt ggagatgagc tggaatgagc
     2041 aaatgctgcc caaatctcaa agtgtaaatg gctataatga aattcagagt ttatatgctg
     2101 gagaaaaatg tggtaatgtc tgggaagaaa atagaagtaa aactgaagac cgacatcctg
     2161 tgtaccatcc tgaagaaggt ggagatgaag gtggaagtgg ttatagtcct ccaagtaaat
     2221 gtgaacagac tgatttatat caccttcctg aaaactttag gccatatacc aatggtcaga
     2281 agcaggaatt taataaccaa gcaaccaatg taattaaatt ttcagatcct caatggaacc
     2341 attttcaggg tcccagttgt caaggtttgg aaccgtataa taaagtgaca tataaacctt
     2401 atcagtcttc tgcccagaat aatggctcac cagcccagga gataacagga agtgacacat
     2461 tcgaaggcct gcaacaacaa tttttaggag ctaatgaaaa ctctgcagaa aatatgcaga
     2521 ttattcaact tcaggttctt aacaaagcaa aagagagaca actggagaac ttaattgaaa
     2581 agttaaatga aagtgaacgt caaattcgat atctgaatca ccagcttgta ataataaaag
     2641 atgaaaagga tggtttgact ctcagccttc gagaatcaca gaaactcttt cagaatggaa
     2701 aagaaagaga gatacagctt gaagctcaaa taaaagcact ggagactcag atacaagcat
     2761 taaaagtcaa tgaagaacag atgatcaaga agtccagaac aactgaaatg gctctggaaa
     2821 gcttgaagca gcagctggtg gaccttcatc attctgaatc acttcaacga gctagagaac
     2881 agcatgagag cattgttatg ggcctcacaa agaagtacga agagcaagta ttgtccttac
     2941 aaaagaattt ggatgccaca gtcaccgcac ttaaagaaca ggaagacatt tgctctcgtc
     3001 tgaaagatca cgtgaaacaa ctggaaagga atcaagaagc aatcaagtta gaaaagactg
     3061 agatcattaa taagttgaca agaagtctag aggagagtca aaagcagtgt gcccacttgt
     3121 tgcagtccgg gtcagtacaa gaggtggctc agctacagtt ccagctgcag caagcacaga
     3181 aggcacatgc tatgagtgca aacatgaaca aggctttgca agaagaatta acagaactaa
     3241 aagatgaaat ttctctctat gaatctgctg caaaactagg aatacatcca agtgactcag
     3301 aaggagaatt aaatatagaa ctcactgaat cgtatgtgga tttggtagga tccatggtga
     3361 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
     3421 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     3481 gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
     3541 tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac ttctttaagc
     3601 agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     3661 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     3721 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     3781 cctccaccga gaccctgtac cccgctgacg gcggcctgga aggcagagcc gacatggccc
     3841 tgaagctcgt gggcgggggc cacctgatct gcaacttgaa gaccacatac agatccaaga
     3901 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacagaaga ctggaaagaa
     3961 tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact
     4021 gcgacctccc tagcaaactg gggcacagat gacccgggcg gccgcgactc tagatcataa
     4081 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     4141 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     4201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4261 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     4321 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4381 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4441 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4501 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4561 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4621 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4681 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4741 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4801 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4861 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     4921 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4981 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5041 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5101 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5161 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5221 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     5281 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5341 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     5401 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     5461 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     5521 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     5581 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5641 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5701 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5761 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     5821 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5881 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5941 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6001 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6061 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     6121 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     6181 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     6241 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     6301 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     6361 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     6421 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     6481 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     6541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7141 gggtcggaac aggagagcgc acgagggagc ttcca
//
