LOCUS       sequence-203028-       12226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, ubiquitous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12226)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 12226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: bactin:mKate2-ras
FEATURES             Location/Qualifiers
     source          1..12226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6651..6675
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6678..7373
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(7453..7477)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7482..7616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7506..7525)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7560..7579
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9756..9943,9944..9955)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9989..10005)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9989..10005)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10042..10062)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10042..10060)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10081..10097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10081..10097)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10094..10116)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10105..10121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10129..10135,10136..10153,10154..10159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10174..10195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10312..10329)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10483..11071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10563..10582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11242..12033,12034..12102))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11865..11884
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12103..12207)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgaaaatt ccagtttgaa gaaacttttc aagactcacc
     1381 ttttcactgt tggtcttaat ttaggttgat tatttttgtc aatttaataa ttacattgta
     1441 ttgttcatat tttattgtct tattgtggtg tttttacagc ttttttattg ttctgctttg
     1501 ttactgtttg ccttgtatgc attgtagcgc tttgggtctg agaaaagcgc actataaata
     1561 aactgtatta ttattattag ggatggacat cacggtggcg cgatgggtgg cacaattacc
     1621 tcacagcaag taggttgctg tttagagccc cggctggatc agttggcagt tctgtgtgga
     1681 gtttgcatgt tctccctgtt caagtaggtt tcctcccggt gctctggttt cccctcacaa
     1741 gtccaaagac atgatgtata ggtgaatggg gtaagctaaa ttgaccatag tgagtgtgtg
     1801 tgtgtgtgtg aatgagagtg tataggtgtt tcccagtgtt aggttgcaac tggaaggaca
     1861 tctgctgtgt aaaacatatg caggataagt tggcggttca ttcccctatg gtgacccctg
     1921 gttaattaaa ggactaagtt gaaaatgaat gaatgaatga atgattagag ataaaccgat
     1981 accacttttt tacaaaccga tatgagtaca agtataatag tttctgttac tcgacaatac
     2041 tgagtaccga ttctgatact tcttggattt ggtttcaatg taattaattt gaaagaaggt
     2101 tttatttctt gcctgtcgca aggatgaaca caaaaatctt taccagaagg atattttaag
     2161 ctaagaatat acgcacgttg ttgacaaccc tgtatcgatt accgattaaa cgtggacatt
     2221 taaaggataa gttatgcttg cgccatcaca actttacaca ttacatgatc aaagatcata
     2281 ttgaatattc ggtttattcg tcagaagcct atagatttgt taaaagtgta ttttataaac
     2341 ggatggttta taaagactaa gctattaata tatcagacat tcattaatac atcaagacat
     2401 cgatgtcagg taggacaaca aatcacatcc atgtaatgaa atcttgttga gtggtattgg
     2461 tgcgtggttt cagcatttca tgtgcaatac gagtaagaat attttaacca agtatcgacc
     2521 caaaacctga tactggtatc ggtgcatccc ttattattat tattatcaaa aacacacttg
     2581 ttttatttat gtgggtgcca aacaacaaac tatgaaatgc agcatgacac gcccaaaaag
     2641 tgttcctttg agaacaactt cataaatatg tttcttatca ttatgcatga atggtgtact
     2701 gggagagctt tggtatcaga attgagataa gagactttgg cattctaaag gacaatgaca
     2761 ttatcacact gacagtcaac aacactttat tgctctggaa atcttatgtc atggggcaaa
     2821 gctgaggttc attaggacag taccttattt agagatgaat gggaaggctg tagtgggctg
     2881 ctatcaggga agggtggtgg tcctcggggg aactatggac tcacaaaagc acatctgctg
     2941 taacaagatc ccctgtgcaa tctacaaaag ttacaaagag aagcatggtt ggttaaaaaa
     3001 tagaattgac tggatatgct gaatgcattg tttgttgtgt gttacatcac attaaccaca
     3061 gattggtgct aaatatttgc ttatcttata aaaacctgtg tgtttataaa ggggggtgtg
     3121 gatggatgct tggcattctt tgaaaagatg ttgggggatg gaaggaggag accttattga
     3181 tgagtatcca gtttgcagat aacaagtttt tagacattca tactatgatg ctgtttatgt
     3241 ctgttttttc tcattttttc tatacacaaa cagctataaa acattcactt ttatatctga
     3301 tgctgtatat taaatttcgt tgtatttatt ctgtttgctc tgttgggtca tagctagggc
     3361 cagacagaat ctgctgacat tttttgcttt ttctgcagag aattttgtaa acaatctgtg
     3421 atttatgcga gattttttcg ggagtatcgt aactaaaaac ttactatatg aaataaaaaa
     3481 aaataatacc tttttaactt ttaataaata tatacaatgc aagtccacct agatccaatt
     3541 atttggtcga caaagcaagt ctctcacata atatatcttc taaaagacag aaaacattac
     3601 tttcagacca tattgtaatc aaatgaacac tttcatatta gtcaataata ttacggaaat
     3661 gtatttaaaa actgaataaa catatattta cacctttgtc tttatttcta gagtgctttt
     3721 acaatgtaga ttgtgtcaaa gcagcttaac ttagaggatt agtatagtaa attgaaactg
     3781 tgtcagtcca ttttttaaga gtttaagttc agttcagttt agttcagtgt gagttaattt
     3841 ttactgctga gagtccaaac actaaagagc aaatccaaat acacaagtca ataaataaac
     3901 tcaatgatgg gctaaagatc tggggaaatc tgcagatttc tgtgcgcaca gattctgtgt
     3961 gggcctagtc attacctata ctcaacgttt actctgagaa acttccatat tttagaggta
     4021 aaatgctcag aattgtaaaa agtcttgtca aaatacttgg aatttgccaa aaacaaaata
     4081 aaagtcacat cacctcattg tccttggtat cagcaagcac ttttaacaca atcagctgtt
     4141 tttgtttata actcccactg aaggccatgt gcattactcc caagttacac agtaaaaagt
     4201 cacatgtagt ttatcatgat cactgctgct caagcacagc tttatctgtt attcagtgtg
     4261 gcaatatctg aaatgtgtgc aaaatattat ttattgtagc tgatcaccaa cactttcagt
     4321 gttttttttt tctactttta atgagaatta tgttcttagg atttatcctt ataaacctct
     4381 ttaaaaagaa atgttatgtc tgcatagaca cgacatgaaa ttatgaacag aaaatttaac
     4441 tatttaagat caagacttga catataacat ttctaatgaa cattaaacct gaggtaggcc
     4501 cacttgcaaa aatgtaactg ctatcactca ctttatacat cattgtgtaa cttgtacaca
     4561 caaaaatagt gaagcaatgt ggcatggaat gcaggtccca ttcattcaaa gttattaaag
     4621 cactttctta aagtgcatta ctactgagct aagaccagct agttgctcat acaacatact
     4681 acaagtagtg tcatcctaat ttggataaaa aaaatgtcca ttttatacat ggaaaagtac
     4741 tcattagatc ccccattgtt tgtattacgg tatttcgtga acacaagagg taaatgacct
     4801 acagagctgc tgttgtgtta gattgaaaac acaacacagg atcatggagg catccatcat
     4861 tatgacgcat tctgccactt gtaactctgc acagactttt tgaaaagttt aattgagtct
     4921 tggcgttgtc gcagaaaaat acgaaaaatt ctgtgttaaa atttgcaaat aaaaattcca
     4981 gttttatagg aaatcgccat ggccttgttc tttaaccagt taagcttccc cttctttcac
     5041 tctcaagttg caagaagcaa gtgtagcaat gtgcacgcga cagccgggtg tgtgacgctg
     5101 gaccaatcag agcacagagc tccgaaagtt taccttttat ggctagagcc gggtatgtgc
     5161 cgtcatataa aagagcgcgc ccagcttttc agcctcactt tgagctcctc cacacgcagc
     5221 tagtgcggaa tatcatctgc ttgtaaccca ttctcttaag tcgacaaccc cccaaaccca
     5281 aggtgagttg gttttacagt ttttaagcgt ttaattatag tttctatcta ttaatgttaa
     5341 gtaaaatgtg taaatggata aatatgattt gatgaagtct ttagacagtt cataacggtc
     5401 tctttgtagt tatctactag gtttgattta taagtggtgg ttcttttgat aatctatgtt
     5461 acatttaata aatattggct gtatcttaac ttttttgtag cgttagtgga tgtggcaggt
     5521 gagaatgtcg gtaatttaac gtgaccagtc gtaggcacga cattgaacat tgaataggcc
     5581 ggtgtgaaat gggtgttaag tcttaatata aagttgtgtt ctggctaatc tctctttaac
     5641 agccataaat gtgattttta actacagttt tgagggtgct ggtgagcatg ttgcacactt
     5701 gatggatagc cggcatggga agttctttgt gcaggcagtg ctgcagcagg gtgtgaccta
     5761 ctttagctag ccggctaacc agctctcatc tgatgtaacg taaaccccat caatctaaca
     5821 tcggctcatt cgtgctttat aagatcgaag atcttgttat tagtaacggg tattggcatt
     5881 tagttttaac ttggttaatt ttctttttgt tatgtgttct atttaaatgt agcctgcagc
     5941 cccctctgtc tttaatgaag ctgaagatta acgttaacgt tagtgaagtg ttgtatccgc
     6001 tatgaaagat ttgaatttta gaggtgtggt ccactttaat aacggctgcg gagttctctg
     6061 gctgcacttc agccgctgtg actgtcgaag cagagcccat cagtttttca ggatctcggt
     6121 gatttttgag gctagactat gaactgaacc gactgagagt gaccgtccag aaatgcttta
     6181 gtgtgaagtc tccgctcacg aggagtccgt ggactgactg cagatctgtg acgcagtaac
     6241 acccggcggc agacacccgt tgaaatgcgg ttgtgtaatt gatcgcaggc gagggtggaa
     6301 gaggatgtga aacttcattt gtgtaaaatt tagggagtgg tcctggtgtg atgaatgtcg
     6361 aaatctgttc ctttttactg agccctacga ctctggctga gtgccacacc gccggcagcc
     6421 gcaaagcggc tcaaaccatt gccttttatg gtaataatga gagaatgcag agggacttcc
     6481 tttgtctggc atatctgagg cgcgcgctgt cactagcgcc caccagcggt cagactgtag
     6541 aatgcagagc aaaacaggaa gttgactcca gatggtcaca tgcttgctga aacgcctctc
     6601 cctggcagca gtgcaattct aacagtttcc ctttctttta cagttcagcc tcaagtttgt
     6661 acaaaaaagc aggctggatg gtgagcgagc tgattaagga gaacatgcac atgaagctgt
     6721 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
     6781 cctacgaggg cacccagacc atgagaatca aggcggtcga gggcggccct ctccccttcg
     6841 ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc
     6901 agggcatccc cgacttcttt aagcagtcct tccccgaggg cttcacatgg gagagagtca
     6961 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     7021 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc
     7081 agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct gacggcggcc
     7141 tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact
     7201 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
     7261 atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc
     7321 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac agaagatctc
     7381 gtggatctaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
     7441 tgctctcctg agacccagct ttcttgtaca aagttgatgt agatccagac atgataagat
     7501 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7561 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7621 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     7681 tcgcggccgc ggcgccaatg cattgggccc tcgagcatat gagatctgtc ccgatctttc
     7741 tcttctgtgc tgtcaattta gagtagtttt tgaggtaatt tgggtgcatt ctctgtaaaa
     7801 acaacaaata aatatttata ttgtatgcaa taaatgtaac aattacacat acactctctg
     7861 attacagcat taaagggtta gttcacccaa aaatgaaaag atgtcattaa tgactcaccc
     7921 tcatgtcgtt ccaagcccgt aacacctccg ttcatcttcg gaacacagtt taagatattt
     7981 tagatttagt ccgagagctt tctgtgcctc cattgagaat gtatgtacgg tatactgtcc
     8041 atgtccagaa aggtaataaa aacatcaaag tagtccatgt gacatcagtg ggttagttag
     8101 aattttttga agcatcgaat acattttggt ccaaaaataa caaaacctac gactttattc
     8161 ggcattgtat tctcttccgg gtctgttgtc aatccgcgtt cacgacttcg cagtgacgct
     8221 acaatgctga ataaagtcgt aggttttgtt atttttggac caaaatgtat tttcgatgct
     8281 tcaaataatt ctacctaacc cactgatgtc acatggacta ctttgatgtt tttattacct
     8341 ttctggacat ggacagtata ccgtacatac attttcagtg gagggacaga aagctctcgg
     8401 actaaatcta aaatatctta aactgtgttc cgaagatgaa cggaggtgtt acgggcttgg
     8461 aacgacatga gggtgagtca ttaatgacat tattttcatt tttgggtgaa ctaacccttt
     8521 aataaaactg acatttgcag ccaattgcac actttataat gttaaatact tgttgatttt
     8581 agaaatacag tagcatttag tgacaatcaa acattaattt tatttctgat tatattgaca
     8641 aaaacacagg actgtaaggc tgacatgaca gggtgaattg atttcattac attagttttg
     8701 atggtaacac ttttacaata aggttcgtta gttaactaca ttagttaaca tgaactaata
     8761 atgaactgca cgtatacatg ttaatttcaa catttactaa tactttatat cttgttaaca
     8821 ttagttaatg cactgtgaac taacatgaac aaacaatgaa catctgtatt tctattaact
     8881 aacgttaaca aagattaaca gatacagtaa caaatgtatt gctcatggtt atttaatgtt
     8941 agttaataca tgaactaatc aacctcattg taaagtgtta ccgttttgac aatacaacat
     9001 gtttggaaaa aaatttctcg cttggttttc ttgtgttttg catgaattca aacacaatga
     9061 gagcacggaa tgataatgat tattcaaagc tgcaataggc gccaaatttc ccgtggacct
     9121 aacgttacca attacaaatg cattttagca ttttattacg tgagccttat attgtcaatc
     9181 gcatgcatta gtgaaaatag gcctactaca catataaaag gcgcgccatc aacgtcaaaa
     9241 catgcacacc caaaaaaaaa aaaaaaaaaa aaaaaaaaac tatcagtaaa acaaaatact
     9301 taatgtactt acctcaatat gcttccttag gtttgatggc gaacttttga aggccgatat
     9361 ttctttatta agcgggagac agaggacaca tttcatcttg aatgaatctt tatttacacc
     9421 acttaaagaa aagaattcgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt
     9481 ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg
     9541 attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat
     9601 gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg
     9661 aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt
     9721 caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact
     9781 ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa
     9841 ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa
     9901 aataatactt aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct
     9961 tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg
    10021 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    10081 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10141 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10201 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10261 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10321 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10381 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10441 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10921 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11221 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11281 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11341 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11401 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11461 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11521 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11581 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11641 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11701 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11761 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11821 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11881 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11941 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12001 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12061 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12121 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12181 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtg
//
