LOCUS       sequence-203030-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing 
            dCas9-VPR and AvrII site for sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Schwartz C, Curtis N, Lobs AK, Wheeldon I
  TITLE     Multiplexed CRISPR activation of cryptic sugar metabolism enables 
            Yarrowia lipolytica growth on cellobiose.
  JOURNAL   Biotechnol J. 2018 May 5:e1700584. doi: 10.1002/biot.201700584.
  PUBMED    29729131
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2018 May 5:e1700584. doi: 10.1002/biot.201700584."
COMMENT     Sequence Label: pCRISPRa_VPR_yl
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1736..1762,1763..1765,1766..4252,4253..4255,
                     4256..5839)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5852..5872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5885..6034
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6053..6073
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6503..6859
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6878..7447
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      7458..7705
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(7474..7492)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(7724..7740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7724..7740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7737..7759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7748..7764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7772..7778,7779..7796,7797..7802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7817..7838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7955..7972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8126..8714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8206..8225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8885..9676,9677..9745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9508..9527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9746..9850)
                     /gene="bla"
                     /label=AmpR promoter
     misc_RNA        10326..10401
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     10451..10469
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt
      421 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca
      481 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat
      541 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat
      601 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggggatccc ccgggttcga
      661 aggtaccaag gaagcatgcg gtacccgaat tcctgaggtg tctcacaagt gccgtgcagt
      721 cccgccccca cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc
      781 cgcccacctc gatccggtct agactgaggt gtctcacaag tgccgtgcag tcccgccccc
      841 acttgcttct ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct
      901 cgatccgggg atccctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc
      961 tctttgtgtg tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggg
     1021 tcgacctgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt
     1081 gtagtgtacg tacattatcg agaccgttgt tcccgcccac ctcgatccgg gagctcctga
     1141 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
     1201 gtacattatc gagaccgttg ttcccgccca cctcgatccg gtctagactg aggtgtctca
     1261 caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat
     1321 cgagaccgtt gttcccgccc acctcgatcc ggggatccct gaggtgtctc acaagtgccg
     1381 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt
     1441 tgttcccgcc cacctcgatc cgggtcgacc tgaggtgtct cacaagtgcc gtgcagtccc
     1501 gcccccactt gcttctcttt gtgtgtagtg tacgtacatt atcgagaccg ttgttcccgc
     1561 ccacctcgat ccgggcatgc ctgcagaagc ttttgtggtt gggactttag ccaagggtat
     1621 aaaagaccac cgtccccgaa ttacctttcc tcttcttttc tctctctcct tgtcaactca
     1681 cacccgaaat cgttaagcat ttccttctga gtataagaat cattcaaagg cgcgcatgga
     1741 taagaaatac tccattggcc tggccatcgg aaccaactcc gtgggttggg ccgtgatcac
     1801 cgatgagtac aaggtgccct ctaagaaatt caaggtcctg ggcaacaccg accgacactc
     1861 catcaagaag aacctgatcg gcgctctgct cttcgactct ggcgagaccg ctgaggccac
     1921 ccgactgaag cgaaccgctc gaagacgata cacccgaaga aagaaccgaa tctgttacct
     1981 gcaggagatc ttctctaacg agatggccaa ggtggacgac tctttcttcc accgactgga
     2041 ggagtctttc ctggtggagg aggacaagaa gcacgagcga caccccatct tcggcaacat
     2101 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctgc gaaagaagct
     2161 ggtggactct accgacaagg ccgacctgcg actgatctac ctggccctgg cccacatgat
     2221 caagttccga ggccacttcc tgatcgaggg cgacctgaac cccgacaact ctgacgtgga
     2281 caagctgttc atccagctgg tgcagaccta caaccagctc ttcgaagaga accccattaa
     2341 cgcttctggc gtggatgcta aggccatcct gtctgcccga ctgtctaagt ctcgacgact
     2401 cgagaacctg attgctcagc tccccggaga gaagaagaac ggtctgttcg gaaacctgat
     2461 tgctctgtcc ctgggtctca cccctaactt caagtccaac ttcgatctgg ctgaggacgc
     2521 taagctgcag ctgtctaagg acacctacga cgatgacctg gataacctgc tcgcccagat
     2581 tggcgaccag tacgccgacc tgttcctggc cgccaagaac ctgtctgacg ccatcctgct
     2641 gtctgacatc ctgcgagtga acaccgagat caccaaggcc cccctgtctg cctccatgat
     2701 taagcgatac gatgagcacc accaggatct gaccctcctc aaggctctgg tccgacagca
     2761 gctgcccgag aagtacaagg agattttctt cgaccagtct aagaacggct acgccggcta
     2821 catcgacggc ggcgcctctc aggaggagtt ctacaagttc attaagccca tcctggagaa
     2881 gatggacgga accgaggaac tgctcgtgaa gctgaaccga gaggacctcc tgcgaaagca
     2941 gcgaaccttc gacaacggct ctatccccca ccagatccac ctgggcgagc tgcacgccat
     3001 cctgcgacga caggaggact tctacccctt cctgaaggac aaccgagaga agatcgagaa
     3061 gatcctgacc ttccgaatcc cctactacgt gggacccctg gcccgaggaa actctcgatt
     3121 cgcttggatg acccgaaagt ctgaggagac cattaccccc tggaacttcg aggaggtggt
     3181 ggataagggc gcctctgctc agtctttcat cgagcgaatg accaacttcg acaagaacct
     3241 ccccaacgag aaggtcctgc ccaagcactc tctgctctac gagtacttca ccgtctacaa
     3301 cgagctcacc aaggtcaagt acgtgaccga gggaatgcga aagcccgctt tcctgtctgg
     3361 agagcagaag aaggctattg tggatctgct cttcaagact aaccgaaagg tcaccgtcaa
     3421 gcagctgaag gaggattact tcaagaagat tgagtgtttc gattctgtcg agatctccgg
     3481 cgtcgaggac cgattcaacg cctctctggg tacctaccac gacctgctga agattatcaa
     3541 ggacaaggat ttcctggata acgaggagaa cgaggatatt ctcgaggaca ttgtcctgac
     3601 cctcaccctg ttcgaggatc gagagatgat tgaggagcga ctcaagacct acgctcacct
     3661 gttcgacgac aaggtgatga agcagctgaa gcgacgacga tacaccggct ggggccgact
     3721 gtctcgaaag ctgatcaacg gcatccgaga caagcagtct ggcaagacca tcctggactt
     3781 cctgaagtct gacggcttcg ccaaccgaaa cttcatgcag ctgatccacg acgactctct
     3841 gaccttcaag gaggacatcc agaaggccca ggtgtctggc cagggcgact ctctgcacga
     3901 gcacatcgcc aacctggccg gctctcccgc cattaagaaa ggtatcctgc agaccgtcaa
     3961 ggtggtcgat gagctcgtca aggtgatggg ccgacacaag cccgagaaca ttgtcattga
     4021 gatggctcga gagaaccaga ctactcagaa gggtcagaaa aactcccgag agcgaatgaa
     4081 gcgaattgag gaaggtatta aggagctggg atcccagatt ctcaaggagc atcccgtgga
     4141 gaacactcag ctccagaacg agaagctgta cctgtactat ctgcagaacg gtcgagacat
     4201 gtacgtcgac caggagctgg atatcaaccg actctccgac tacgatgtgg acgccattgt
     4261 gccccagtcc ttcctgaagg acgattctat cgataacaag gtgctgaccc gatccgacaa
     4321 gaaccgaggc aagtctgaca acgtgccctc cgaggaggtg gtcaagaaga tgaagaacta
     4381 ctggcgacag ctgctgaacg ccaagctgat tacccagcga aagttcgaca acctgaccaa
     4441 ggccgagcga ggcggcctgt ctgagctgga caaggccggc ttcatcaagc gacagctggt
     4501 ggagacccga cagatcacca agcacgtggc ccagatcctg gactctcgaa tgaacaccaa
     4561 gtacgacgag aacgacaagc tgatccgaga ggtgaaggtg atcaccctga agtctaagct
     4621 ggtgtctgac ttccgaaagg acttccagtt ctacaaggtg cgagagatta acaactacca
     4681 ccacgcccac gatgcctacc tgaacgctgt cgtgggcacc gccctcatca agaagtatcc
     4741 caagctggag tccgagttcg tctacggcga ctacaaggtc tacgatgtgc gaaaaatgat
     4801 tgccaagtcc gagcaggaga ttggcaaggc taccgccaag tacttcttct actccaacat
     4861 tatgaacttc ttcaagaccg agattaccct ggctaacggc gagattcgaa agcgacccct
     4921 cattgagacc aacggagaga ccggtgagat cgtgtgggac aagggacgag acttcgccac
     4981 cgtgcgaaag gtgctgtcta tgccccaggt gaacatcgtg aagaagaccg aggtgcagac
     5041 cggaggtttc tctaaggagt ccatcctgcc caagcgaaac tctgacaagc tgatcgcccg
     5101 aaagaaggac tgggacccca agaagtacgg aggtttcgac tctcccaccg tggcttactc
     5161 tgtgctggtg gtggccaagg tggagaaggg caagtctaag aagctgaagt ctgtgaagga
     5221 gctgctgggc attaccatca tggagcgatc ttctttcgag aagaacccca ttgacttcct
     5281 ggaggccaag ggatacaagg aggtgaagaa agatctgatt atcaagctcc ccaagtactc
     5341 tctgttcgag ctggagaacg gacgaaagcg aatgctggcc tctgccggcg agctgcagaa
     5401 gggaaacgag ctggccctgc cctccaagta cgtcaacttc ctgtacctcg cctcccatta
     5461 cgagaagctg aagggctctc ccgaggataa cgagcagaag cagctcttcg tggagcagca
     5521 taagcactac ctggacgaga tcatcgagca gatctctgag ttctctaagc gagtgatcct
     5581 ggctgacgcc aacctggata aggtgctgtc tgcttacaac aagcaccgag acaagcccat
     5641 tcgagagcag gctgagaaca tcattcacct gttcaccctg accaacctgg gagcccccgc
     5701 tgccttcaag tacttcgaca ccaccatcga ccgaaagcga tacacctcta ccaaggaggt
     5761 gctggacgcc accctgatcc accagtctat caccggcctg tacgagaccc gaatcgacct
     5821 gtctcagctg ggcggcgact ctcgagccga ccccaagaag aagcgaaagg tgggaggatc
     5881 aggagacgct ctcgatgact ttgatctgga catgcttggc tccgatgctc ttgacgattt
     5941 cgacctcgac atgcttggat ctgacgctct ggatgacttc gaccttgata tgctcggatc
     6001 tgatgccctt gacgatttcg atctggacat gctgccgcga tcttctggct ctcccaagaa
     6061 gaagcgaaag gtgggctctc agtacctgcc cgacaccgac gaccgacacc gaatcgagga
     6121 gaagcgaaag cgaacctacg agaccttcaa gtctatcatg aagaagtctc ccttctctgg
     6181 ccctaccgac ccacgacctc ctccccgacg aatcgccgtg ccctctcgat cttctgcctc
     6241 tgtgcccaag cccgctcctc agccctaccc cttcacctct tctctgtcta ccatcaacta
     6301 cgacgagttc cccaccatgg tgttcccctc tggccagatc tctcaggcct ctgccctggc
     6361 accagctcct ccacaggtgc tgcctcaggc acctgcaccg gctcctgcac cggccatggt
     6421 gtctgccctg gcccaagccc ctgctcccgt gcctgtgctg gcacctggcc ctccccaagc
     6481 tgtggctcct ccggctccta agcccaccca ggccggcgag ggcaccctgt ctgaggctct
     6541 gctgcagctg cagttcgacg acgaggacct gggcgccctg ctgggcaact ctaccgaccc
     6601 cgccgtgttc accgacctgg cctctgtgga caactctgag ttccagcagc tgctgaacca
     6661 gggcatcccc gtggctcctc acaccaccga gcccatgctg atggagtacc ccgaggccat
     6721 cacccgactg gtgaccggcg cccagcgacc tccagacccc gctcctgccc ctctgggcgc
     6781 ccccggcctg cccaacggcc tgctgtctgg cgacgaggac ttctcttcta tcgccgacat
     6841 ggacttctct gccctgctgg gctctggctc tggctctcga gactctcgag agggcatgtt
     6901 cctgcccaag cccgaggccg gctctgccat ctctgacgtg ttcgagggcc gagaggtgtg
     6961 ccagcccaag cgaatccgac ccttccaccc acccggctct ccttgggcta accgacccct
     7021 gcctgcttct ctggcaccaa cccccaccgg ccctgtgcac gagccagtgg gctctctgac
     7081 ccctgctcca gtgccccagc ctctggaccc tgcccccgct gtgacccccg aggcctctca
     7141 cctgctggag gaccccgacg aggagacctc tcaggccgtg aaggccctgc gagagatggc
     7201 cgacaccgtg atcccccaga aggaggaggc cgccatctgc ggccagatgg acctgtctca
     7261 ccctccaccc cgaggccacc tggacgagct gaccaccacc ctggagtcta tgaccgagga
     7321 cctgaacctg gactctcccc tgacccccga gctgaacgag atcctggaca ccttcctgaa
     7381 cgacgagtgc ctgctgcacg ccatgcacat ctctaccggc ctgtctatct tcgacacctc
     7441 tctgttctaa gctagcctca tgtaattagt tatgtcacgc ttacattcac gccctccccc
     7501 cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta
     7561 tttttttata gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt
     7621 tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt
     7681 gggacgctcg aaggctttaa tttgcaagct tggcgtaatc atggtcatag ctgtttcctg
     7741 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7801 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7861 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7921 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7981 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8041 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8101 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8161 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8221 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8281 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     8341 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8401 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8461 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8521 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     8581 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8641 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8701 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8761 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8821 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8881 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8941 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     9001 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     9061 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9121 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9181 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9241 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9301 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9361 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9421 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9481 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9541 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9601 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9661 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9721 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9781 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9841 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ccccagttgc aaaagttgac
     9901 acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg tgattatata
     9961 atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac gctctaatgc
    10021 ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt gtatgagact
    10081 ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta aacaaagttt
    10141 tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt gtgcgttcga
    10201 gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga tggtttagtg
    10261 gtaaaatcca tcgttgccat cgatgggccc ccggttcgat tccgggtcgg cgcaggttga
    10321 cctaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
    10381 aaagtggcac cgagtcggtg ctttttttta cgtctaagaa accattatta tcatgacatt
    10441 aacctataaa aataggcgta tcacgaggcc cagatcctct agagtcgaag cggccgctat
    10501 gtctgataaa aggatgtaac ataggcaagc tgctcgtgag tgttgagtac gaaccttaga
    10561 tccaaatcac ccgcacccac ggatatactt gcttgaatat acagtagtat gcggccgctt
    10621 cgacaccata tcatataaaa ctaacaatgc attgcttatt acgaagacta cccgttgcta
    10681 tctccacacc gttatctcca cggtccaaag gctgctcaat gtgctgcata cgtaacgtgg
    10741 ggtgcaacct tgagcacata gtacttttcc gaaaaccggc gataattaag tgtgcactcc
    10801 aacttttcac actgagcgta aaatgtggag aagaaatcgg cactaaaaag tcaggtagac
    10861 tggaaaatgc gccatgaaat gaatatctct tgctacagta atgcccagca tcgaggggta
    10921 ttgtgtcacc aacactatag tggcagctga agcgctcgtg attgtagtat gagtctttat
    10981 tggtgatggg aagagttcac tcaatattct cgttactgcc aaaacaccac ggtaatcggc
    11041 cagacaccat ggatgtagat caccaagcct gtgaatgtta ttcgagctaa aatgcacatg
    11101 gttggtgaaa ggagtagttg ctgtcgaatt ccgtcgtcgc ctgagtcatc atttatttac
    11161 cagttggcca caaacccttg acgatctcgt atgtcccctc cgacatactc ccggccggct
    11221 ggggtacgtt cgatagcgct atcggcatcg acaaggtttg ggtccctagc cgataccgca
    11281 ctacctgagt cacaatcttc ggaggtttag tcttccacat agcacgggca aaagtgcgta
    11341 tatatacaag agcgtttgcc agccacagat tttcactcca cacaccacat cacacataca
    11401 accacacaca tccacaatgg aacccgaaac taagaagacc aagactgact ccaagaagat
    11461 tgttcttctc ggcggcgact tctgtggccc cgaggtgatt gccgaggccg tcaaggtgct
    11521 caagtctgtt gctgaggcct ccggcaccga gtttgtgttt gaggaccgac tcattggagg
    11581 agctgccatt gagaaggagg gcgagcccat caccgacgct actctcgaca tctgccgaaa
    11641 ggctgactct attatgctcg gtgctgtcgg aggcgctgcc aacaccgtat ggaccactcc
    11701 cgacggacga accgacgtgc gacccgagca gggtctcctc aagctgcgaa aggacctgaa
    11761 cctgtacgcc aacctgcgac cctgccagct gctgtcgccc aagctcgccg atctctcccc
    11821 catccgaaac gttgagggca ccgacttcat cattgtccga gagctcgtcg gaggtatcta
    11881 ctttggagag cgaaaggagg atgacggatc tggcgtcgct tccgacaccg agacctactc
    11941 cgttcctgag gttgagcgaa ttgcccgaat ggccgccttc ctggcccttc agcacaaccc
    12001 ccctcttccc gtgtggtctc ttgacaaggc caacgtgctg gcctcctctc gactttggcg
    12061 aaagactgtc actcgagtcc tcaaggacga attcccccag ctcgagctca accaccagct
    12121 gatcgactcg gccgccatga tcctcatcaa gcagccctcc aagatgaatg gtatcatcat
    12181 caccaccaac atgtttggcg atatcatctc cgacgaggcc tccgtcatcc ccggttctct
    12241 gggtctgctg ccctccgcct ctctggcttc tctgcccgac accaacgagg cgttcggtct
    12301 gtacgagccc tgtcacggat ctgcccccga tctcggcaag cagaaggtca accccattgc
    12361 caccattctg tctgccgcca tgatgctcaa gttctctctt aacatgaagc ccgccggtga
    12421 cgctgttgag gctgccgtca aggagtccgt cgaggctggt atcactaccg ccgatatcgg
    12481 aggctcttcc tccacctccg aggtcggaga cttgttgcca acaaggtcaa ggagctgctc
    12541 aagaaggagt aagtcgtttc tacgacgcat tgatggaagg agcaaactga cgcgcctgcg
    12601 ggttggtcta ccggcagggt ccgctagtgt ataagactct ataaaaaggg ccctgccctg
    12661 ctaatgaaat gatgatttat aatttaccgg tgtagcaacc ttgactagaa gaagcagatt
    12721 gggtgtgttt gtagtggagg acagtggtac gttttggaaa cagtcttctt gaaagtgtct
    12781 tgtctacagt atattcactc ataacctcaa tagccaaggg tgtagtcggt ttattaaagg
    12841 aagggagttg tggctgatgt ggatagatat ctttaagctg gcgactgcac ccaacgagtg
    12901 tggtggtagc ttgttactgt atattcggta agatatattt tgtggggttt tagtggtgtt
    12961 tggtaggtta gtgcttggta tatgagttgt aggcatgaca atttggaaag gggtggactt
    13021 tgggaatatt gtgggatttc aataccttag tttgtacagg gtaattgtta caaatgatac
    13081 aaagaactgt atttcttttc atttgtttta attggttgta tatcaagtcc gttagacgag
    13141 ctcagtgcca tggcttttgg cactgtattt catttttaga ggtacactac attcagtgag
    13201 gtatggtaag gttgagggca taatgaaggc accttgtact gacagtcaca gacctctcac
    13261 cgagaatttt atgagatata ctcgggttca ttttaggctc cgattcgatt caaattatta
    13321 ctgtcgaaat cggttgagca tccgttgatt tccgaacaga tctggccctt tcgtc
//
