LOCUS       sequence-203036-       11344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HA-CRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11344)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-HA-CRBN-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..47
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1588..2087
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1623..1646)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2020..2039
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2105..3130
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2105..3130
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3161..3194)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3250..3838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3303..3323)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3721..3732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3841..3857)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3842..3858)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4047..4280
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4352..4473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4389..4408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4443..4462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4513..4648
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4668..4687)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4669..4687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4710..5068)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4750..4771)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4962..4981
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5095..5199
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5201..5558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5201..5392
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5224..5244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5409..5544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5593..6387
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5593..6387
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5647..5666)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6257..6276
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6575..6594)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6619..6666
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6995..7583
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7484..7503
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7758..8061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8063..8261
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8216..8236
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8279..8459
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8506..8631
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9130..9363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9548..9592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9890..10007
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10099..11310
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11147..11166
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gatccagatc tgccaccatg tacccatacg atgttccaga ttacgctggc ggagctcgag
       61 gttatcaaac aagtttgtac aaaaaagttg gcatggccgg cgaaggagat cagcaggacg
      121 ctgcgcacaa catgggcaac cacctgccgc tcctgcctga gagtgaggaa gaagatgaaa
      181 tggaagttga agaccaggat agtaaagaag ccaaaaaacc aaacatcata aattttgaca
      241 ccagtctgcc gacatcacat acatacctag gtgctgatat ggaagaattt catggcagga
      301 ctttgcacga tgacgacagc tgtcaggtga ttccagttct tccacaagtg atgatgatcc
      361 tgattcccgg acagacatta cctcttcagc tttttcaccc tcaagaagtc agtatggtgc
      421 ggaatttaat tcagaaagat agaacctttg ctgttcttgc atacagcaat gtacaggaaa
      481 gggaagcaca gtttggaaca acagcagaga tatatgccta tcgagaagaa caggattttg
      541 gaattgagat agtgaaagtg aaagcaattg gaagacaaag gttcaaagtc cttgagctaa
      601 gaacacagtc agatggaatc cagcaagcta aagtgcaaat tcttcccgaa tgtgtgttgc
      661 cttcaaccat gtctgcagtt caattagaat ccctcaataa gtgccagata tttccttcaa
      721 aacctgtctc aagagaagac caatgttcat ataaatggtg gcagaaatac cagaagagaa
      781 agtttcattg tgcaaatcta acttcatggc ctcgctggct gtattcctta tatgatgctg
      841 agaccttaat ggacagaatc aagaaacagc tacgtgaatg ggatgaaaat ctaaaagatg
      901 attctcttcc ttcaaatcca atagattttt cttacagagt agctgcttgt cttcctattg
      961 atgatgtatt gagaattcag ctccttaaaa ttggcagtgc tatccagcga cttcgctgtg
     1021 aattagacat tatgaataaa tgtacttccc tttgctgtaa acaatgtcaa gaaacagaaa
     1081 taacaaccaa aaatgaaata ttcagtttat ccttatgtgg gccgatggca gcttatgtga
     1141 atcctcatgg atatgtgcat gagacactta ctgtgtataa ggcttgcaac ttgaatctga
     1201 taggccggcc ttctacagaa cacagctggt ttcctgggta tgcctggact gttgcccagt
     1261 gtaagatctg tgcaagccat attggatgga agtttacggc caccaaaaaa gacatgtcac
     1321 ctcaaaaatt ttggggctta acgcgatctg ctctgttgcc cacgatccca gacactgaag
     1381 atgaaataag tccagacaaa gtaatacttt gcttgtaaga cccagattta ttgtacaaag
     1441 tggttcgata acgaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     1501 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     1561 tgagggccgc gtttaacgaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     1621 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     1681 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     1741 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     1801 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     1861 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     1921 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     1981 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     2041 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcatcccgc
     2101 caccatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     2161 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     2221 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     2281 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     2341 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     2401 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     2461 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     2521 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     2581 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     2641 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     2701 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     2761 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     2821 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     2881 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     2941 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     3001 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     3061 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     3121 aaaggaatag tgtacaagta ggaattcgtc gagggaccta ataacttcgt atagcataca
     3181 ttatacgaag ttatacatgt ttaagggttc cggttccact aggtacaatt cgatatcaag
     3241 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3301 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3361 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3421 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3481 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3541 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3601 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     3661 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3721 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3781 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     3841 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     3901 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     3961 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     4021 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     4081 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4141 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4201 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4261 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     4321 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     4381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     4501 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4561 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4621 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     4681 cgtattagca tgtgagcaaa aggccagcat aaatcaaaag aatagaccga gatagggttg
     4741 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     4801 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     4861 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     4921 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     4981 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     5041 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5161 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     5221 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5281 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5341 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5401 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5461 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5521 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     5581 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     5641 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     5701 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     5761 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     5821 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     5881 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     5941 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     6001 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     6061 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     6121 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     6181 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     6241 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     6301 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     6361 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     6421 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6481 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6541 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     6601 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     6661 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     6721 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     6781 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     6841 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6901 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6961 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7021 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7081 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7141 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7201 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7261 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7321 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7381 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7441 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7501 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7561 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7621 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7681 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     7741 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     7801 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     7861 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7921 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     7981 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8041 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8101 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8161 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8221 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     8281 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8341 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8401 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8461 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8521 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8581 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     8641 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8701 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8761 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8821 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8881 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8941 aacaaaagta agaccaccgc acagcaagcg gccggccgcg ctgatcttca gacctggagg
     9001 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9061 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9121 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9181 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9241 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9301 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9361 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9421 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9481 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9541 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9601 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9661 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9721 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     9781 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     9841 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     9901 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9961 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
    10021 gggacagcag agatccagtt tggttagtac cgggcccgct ctagccctag atttaaccca
    10081 acagaaggct cgacgcgtgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
    10141 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
    10201 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
    10261 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
    10321 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
    10381 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
    10441 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
    10501 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
    10561 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
    10621 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
    10681 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
    10741 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
    10801 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
    10861 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
    10921 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
    10981 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
    11041 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
    11101 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
    11161 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
    11221 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
    11281 ttctggccgt ttttggcttt tttgttagac gctagactgc cggatcgaat tgtcgacgaa
    11341 ttcg
//
