LOCUS       sequence-203038-       11347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of myc-CRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-Myc-CRBN-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..83
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1624..2123
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1659..1682)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2056..2075
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2141..3166
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2141..3166
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3197..3230)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3286..3874
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3339..3359)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3757..3768)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3877..3893)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3878..3894)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4083..4316
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4388..4509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4425..4444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4479..4498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4549..4684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4704..4723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4705..4723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4746..5104)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4786..4807)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4998..5017
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5131..5235
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5237..5594
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5237..5428
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5260..5280)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5445..5580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5629..6423
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5629..6423
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5683..5702)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6293..6312
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6611..6630)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6655..6702
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7031..7619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7520..7539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7794..8097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8099..8297
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8252..8272
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8315..8495
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8542..8667
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9166..9399
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9584..9628
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9926..10043
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10135..11346
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11183..11202
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ctagactgcc ggatcgaatt gtcgacgaat tcggatccag atctgccacc atggagcaga
       61 agctgatctc tgaggaggat ctgggaggcg ctcgaggtta tcaaacaagt ttgtacaaaa
      121 aagttggcat ggccggcgaa ggagatcagc aggacgctgc gcacaacatg ggcaaccacc
      181 tgccgctcct gcctgagagt gaggaagaag atgaaatgga agttgaagac caggatagta
      241 aagaagccaa aaaaccaaac atcataaatt ttgacaccag tctgccgaca tcacatacat
      301 acctaggtgc tgatatggaa gaatttcatg gcaggacttt gcacgatgac gacagctgtc
      361 aggtgattcc agttcttcca caagtgatga tgatcctgat tcccggacag acattacctc
      421 ttcagctttt tcaccctcaa gaagtcagta tggtgcggaa tttaattcag aaagatagaa
      481 cctttgctgt tcttgcatac agcaatgtac aggaaaggga agcacagttt ggaacaacag
      541 cagagatata tgcctatcga gaagaacagg attttggaat tgagatagtg aaagtgaaag
      601 caattggaag acaaaggttc aaagtccttg agctaagaac acagtcagat ggaatccagc
      661 aagctaaagt gcaaattctt cccgaatgtg tgttgccttc aaccatgtct gcagttcaat
      721 tagaatccct caataagtgc cagatatttc cttcaaaacc tgtctcaaga gaagaccaat
      781 gttcatataa atggtggcag aaataccaga agagaaagtt tcattgtgca aatctaactt
      841 catggcctcg ctggctgtat tccttatatg atgctgagac cttaatggac agaatcaaga
      901 aacagctacg tgaatgggat gaaaatctaa aagatgattc tcttccttca aatccaatag
      961 atttttctta cagagtagct gcttgtcttc ctattgatga tgtattgaga attcagctcc
     1021 ttaaaattgg cagtgctatc cagcgacttc gctgtgaatt agacattatg aataaatgta
     1081 cttccctttg ctgtaaacaa tgtcaagaaa cagaaataac aaccaaaaat gaaatattca
     1141 gtttatcctt atgtgggccg atggcagctt atgtgaatcc tcatggatat gtgcatgaga
     1201 cacttactgt gtataaggct tgcaacttga atctgatagg ccggccttct acagaacaca
     1261 gctggtttcc tgggtatgcc tggactgttg cccagtgtaa gatctgtgca agccatattg
     1321 gatggaagtt tacggccacc aaaaaagaca tgtcacctca aaaattttgg ggcttaacgc
     1381 gatctgctct gttgcccacg atcccagaca ctgaagatga aataagtcca gacaaagtaa
     1441 tactttgctt gtaagaccca gatttattgt acaaagtggt tcgataacga attccgcccc
     1501 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     1561 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggccgcgttt aacgaattct
     1621 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     1681 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     1741 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     1801 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     1861 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     1921 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     1981 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     2041 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     2101 cttcctcatc tccgggcctt tcgacctgca tcccgccacc atgaaaaagc ctgaactcac
     2161 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     2221 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2281 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     2341 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     2401 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     2461 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
     2521 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     2581 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     2641 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     2701 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     2761 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     2821 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     2881 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     2941 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     3001 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     3061 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     3121 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagtgta caagtaggaa
     3181 ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa
     3241 gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca acctctggat
     3301 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3361 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3421 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3481 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3541 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3601 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3661 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3721 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3781 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3841 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga
     3901 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     3961 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     4021 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4081 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4141 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4201 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4261 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4321 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4381 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4501 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     4561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4681 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttagcatgtg agcaaaaggc
     4741 cagcataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4801 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4861 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4921 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4981 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     5041 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     5101 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5221 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     5281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5581 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5641 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5701 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5761 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
     5821 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5881 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5941 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6001 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6061 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6121 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
     6181 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6241 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6301 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6361 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6421 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6481 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6541 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
     6601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
     6661 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
     6721 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
     6781 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
     6841 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
     6901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat
     7741 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7801 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7861 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7921 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7981 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     8041 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     8101 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     8161 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     8221 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     8281 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
     8341 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8401 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8461 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8521 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8581 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8641 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8701 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8761 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8821 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8881 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8941 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9001 caagcggccg gccgcgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     9061 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9121 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9181 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9241 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9301 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9361 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9421 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9481 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9541 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9601 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9661 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9721 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9781 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     9841 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca
     9901 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9961 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10021 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt
    10081 tagtaccggg cccgctctag ccctagattt aacccaacag aaggctcgac gcgtggcctc
    10141 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
    10201 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
    10261 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
    10321 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
    10381 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
    10441 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
    10501 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
    10561 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
    10621 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
    10681 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
    10741 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
    10801 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
    10861 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
    10921 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
    10981 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
    11041 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
    11101 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
    11161 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
    11221 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
    11281 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
    11341 ttagacg
//
