LOCUS       sequence-20304-e         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Clark EA, Golub TR, Lander ES, Hynes RO
  TITLE     Genomic analysis of metastasis reveals an essential role for RhoC.
  JOURNAL   Nature. 2000 Aug 3. 406(6795):532-5.
  PUBMED    10952316
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2000 Aug 3. 406(6795):532-5."
COMMENT     Sequence Label: pMIG-N19RhoA
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..390
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     328..344
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             427..456
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gcgcccccat tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
       61 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
      121 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
      181 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
      241 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
      301 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
      361 gaccccgggg gtggaccatc ctctagactg ccggatccca gtgtggtggt acgggaattc
      421 cccatggagc agaagctgat ctccgaggag gacctgaacc ggaagaaact ggtgattgtt
      481 ggtgatggag cctgtggaaa gaactgcttg ctcatagtct tcagcaagga ccagttccca
      541 gaggtgtatg tgcccacagt gtttgagaac tatgtggcag atatcgaggt ggatgga
//
