LOCUS       sequence-203040-       11341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of FLAG-CRBN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UbcP-FLAG-CRBN-pGK-HYG
FEATURES             Location/Qualifiers
     source          1..11341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1585..2084
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1620..1643)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2017..2036
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2102..3127
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2102..3127
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(3158..3191)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3247..3835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3300..3320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3718..3729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3838..3854)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3839..3855)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4044..4277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4349..4470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4386..4405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4440..4459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4510..4645
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4665..4684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4666..4684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4707..5065)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4747..4768)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4959..4978
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5092..5196
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5198..5555
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5198..5389
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5221..5241)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5406..5541
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5590..6384
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5590..6384
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5644..5663)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6254..6273
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6572..6591)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6616..6663
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6992..7580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7481..7500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7755..8058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8060..8258
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8213..8233
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8276..8456
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8503..8628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9127..9360
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9545..9589
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9887..10004
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10096..11307
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     11144..11163
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gatccagatc tgccaccatg gactacaagg acgacgatga caagggcgga gctcgaggtt
       61 atcaaacaag tttgtacaaa aaagttggca tggccggcga aggagatcag caggacgctg
      121 cgcacaacat gggcaaccac ctgccgctcc tgcctgagag tgaggaagaa gatgaaatgg
      181 aagttgaaga ccaggatagt aaagaagcca aaaaaccaaa catcataaat tttgacacca
      241 gtctgccgac atcacataca tacctaggtg ctgatatgga agaatttcat ggcaggactt
      301 tgcacgatga cgacagctgt caggtgattc cagttcttcc acaagtgatg atgatcctga
      361 ttcccggaca gacattacct cttcagcttt ttcaccctca agaagtcagt atggtgcgga
      421 atttaattca gaaagataga acctttgctg ttcttgcata cagcaatgta caggaaaggg
      481 aagcacagtt tggaacaaca gcagagatat atgcctatcg agaagaacag gattttggaa
      541 ttgagatagt gaaagtgaaa gcaattggaa gacaaaggtt caaagtcctt gagctaagaa
      601 cacagtcaga tggaatccag caagctaaag tgcaaattct tcccgaatgt gtgttgcctt
      661 caaccatgtc tgcagttcaa ttagaatccc tcaataagtg ccagatattt ccttcaaaac
      721 ctgtctcaag agaagaccaa tgttcatata aatggtggca gaaataccag aagagaaagt
      781 ttcattgtgc aaatctaact tcatggcctc gctggctgta ttccttatat gatgctgaga
      841 ccttaatgga cagaatcaag aaacagctac gtgaatggga tgaaaatcta aaagatgatt
      901 ctcttccttc aaatccaata gatttttctt acagagtagc tgcttgtctt cctattgatg
      961 atgtattgag aattcagctc cttaaaattg gcagtgctat ccagcgactt cgctgtgaat
     1021 tagacattat gaataaatgt acttcccttt gctgtaaaca atgtcaagaa acagaaataa
     1081 caaccaaaaa tgaaatattc agtttatcct tatgtgggcc gatggcagct tatgtgaatc
     1141 ctcatggata tgtgcatgag acacttactg tgtataaggc ttgcaacttg aatctgatag
     1201 gccggccttc tacagaacac agctggtttc ctgggtatgc ctggactgtt gcccagtgta
     1261 agatctgtgc aagccatatt ggatggaagt ttacggccac caaaaaagac atgtcacctc
     1321 aaaaattttg gggcttaacg cgatctgctc tgttgcccac gatcccagac actgaagatg
     1381 aaataagtcc agacaaagta atactttgct tgtaagaccc agatttattg tacaaagtgg
     1441 ttcgataacg aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag
     1501 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     1561 gggccgcgtt taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
     1621 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
     1681 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
     1741 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
     1801 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
     1861 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
     1921 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
     1981 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
     2041 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atcccgccac
     2101 catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     2161 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     2221 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     2281 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     2341 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     2401 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     2461 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     2521 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     2581 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     2641 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     2701 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     2761 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     2821 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     2881 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     2941 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     3001 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     3061 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     3121 ggaatagtgt acaagtagga attcgtcgag ggacctaata acttcgtata gcatacatta
     3181 tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga tatcaagctt
     3241 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3661 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3841 taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     3901 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     3961 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4021 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     4081 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4141 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4201 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4261 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     4321 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     4381 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4441 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     4501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4561 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4621 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     4681 attagcatgt gagcaaaagg ccagcataaa tcaaaagaat agaccgagat agggttgagt
     4741 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4801 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4861 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4921 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4981 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     5041 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5161 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
     5221 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5281 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5341 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5401 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5461 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5521 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
     5581 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5641 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5701 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5761 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5821 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5881 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5941 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6001 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6061 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     6121 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6181 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6241 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6301 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6361 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6421 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6481 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6541 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     6601 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
     6661 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
     6721 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
     6781 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
     6841 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6901 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     7141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7681 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     7741 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     7801 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     7861 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     7921 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     7981 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     8041 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     8101 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     8161 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     8221 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     8281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     8461 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8521 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8581 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8641 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8701 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8761 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8821 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8881 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8941 aaaagtaaga ccaccgcaca gcaagcggcc ggccgcgctg atcttcagac ctggaggagg
     9001 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9061 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9121 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9181 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9241 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9301 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9361 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9421 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9481 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9541 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9601 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9661 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9721 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     9781 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     9841 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9901 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9961 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10021 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gccctagatt taacccaaca
    10081 gaaggctcga cgcgtggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
    10141 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
    10201 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
    10261 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
    10321 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
    10381 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
    10441 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
    10501 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
    10561 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
    10621 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
    10681 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
    10741 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
    10801 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
    10861 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
    10921 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
    10981 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
    11041 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
    11101 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
    11161 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
    11221 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
    11281 tggccgtttt tggctttttt gttagacgct agactgccgg atcgaattgt cgacgaattc
    11341 g
//
