LOCUS       sequence-203041-        5685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry clone for gateway cloning, p150Glued subunit of dynactin, 
            cofactor of dynein, dominant negative truncation, amino acids 1-811.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, 
            Norden C, Wilsch-Brauninger M, Huttner WB
  TITLE     Non-canonical features of the Golgi apparatus in bipolar epithelial 
            neural stem cells.
  JOURNAL   Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
  PUBMED    26879757
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:21206. doi"; pages: " 
            10.1038/srep21206"
COMMENT     Sequence Label: pME mKate2-dynactin1-811
FEATURES             Location/Qualifiers
     source          1..5685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             671..1366
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(3810..3909)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3926..3945)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3927..3945)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3950..3966)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3950..3966)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4068..4087)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4079..4888
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4146..4165)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5035..5623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5524..5543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg atggtgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga
      721 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
      781 gggcacccag accatgagaa tcaaggcggt cgagggcggc cctctcccct tcgccttcga
      841 catcctggct accagcttca tgtacggcag caaaaccttc atcaaccaca cccagggcat
      901 ccccgacttc tttaagcagt ccttccccga gggcttcaca tgggagagag tcaccacata
      961 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     1021 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1081 aacactcggc tgggaggcct ccaccgagac cctgtacccc gctgacggcg gcctggaagg
     1141 cagagccgac atggccctga agctcgtggg cgggggccac ctgatctgca acttgaagac
     1201 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     1261 cagaagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     1321 ggctgtggcc agatactgcg acctccctag caaactgggg cacagagata tcatggcaca
     1381 gagcaagagg cacgtgtaca gccggacgcc cagcggcagc aggatgagtg cggaggcaag
     1441 cgcccggcct ctgcgggtgg gctcccgtgt agaggtgatt ggaaaaggcc accgaggcac
     1501 tgtggcctat gttggagcca cactgtttgc cactggcaaa tgggtaggca tgattctgga
     1561 tgaagcaaag ggcaaaaatg atggaactgt tcaaggcagg aagtacttca cttgtgatga
     1621 agggcatggc atctttgtgc gccagtccca gatccaggta tttgaagatg gagcagatac
     1681 tacttcccca gagacacctg attcttctgc ttcaaaagtc ctcaaaagag agggaactga
     1741 tacaactgca aagactagca aactgcgggg actgaagcct aagaaggcac cgacagcccg
     1801 aaagaccaca actcggcgac ccaagcccac gcgcccagcc agtactgggg tggctggggc
     1861 cagtagctcc ctgggcccct ctggctcagc gtcagcaggt gagctgagca gcagtgagcc
     1921 cagcaccccg gctcagactc cgctggcagc acccatcatc cccacgccgg tcctcacctc
     1981 tcctggagca gtccccccgc ttccttcccc atccaaggag gaggagggac taagggctca
     2041 ggtgcgggac ctggaggaga aactagagac cctgagactg aaacgggcag aagacaaagc
     2101 aaagctaaaa gagctggaga aacacaaaat ccagctggag caggtgcagg aatggaagag
     2161 caaaatgcag gagcagcagg ccgacctgca gcggcgcctc aaggaggcga gaaaggaagc
     2221 caaggaggcg atggaggcaa aggaacgcta tatggaggag atggctgata ctgctgatgc
     2281 cattgagatg gccactttgg acaaggagat ggctgaagag cgggctgagt ccctgcagca
     2341 ggaggtggag gcactgaagg agcgggtgga cgagctcact actgacttag agatcctcaa
     2401 ggctgagatt gaagagaagg gctcagatgg cgctgcatcc agttatcagc tcaagcagct
     2461 tgaggagcag aatgcccgcc tgaaggatgc cctggtgagg atgcgggatc tttcttcctc
     2521 agagaagcag gagcatgtga agctccagaa gctcatggaa aagaagaacc aagagctgga
     2581 agttgtgagg caacagcggg agcgtctgca ggaggagcta agccaggcag agagcaccat
     2641 tgatgagctc aaggagcagg tggatgctgc tctgggtgct gaggagatgg tggagatgct
     2701 gacagatcgg aacctgaatc tggaagagaa agtgcgcgag ttgagggaga ctgtgggaga
     2761 cttggaagcg atgaatgaga tgaacgatga gctgcaggag aatgcacgtg agacagaact
     2821 ggagctgcgg gagcagctgg acatggcagg cgcgcgggtt cgtgaggccc agaagcgtgt
     2881 ggaggcagcc caggagacgg ttgcagacta ccagcagacc atcaagaagt accgccagct
     2941 gaccgcccat ctacaggatg tgaatcggga actgacgaac cagcaggaag catctgtgga
     3001 gaggcaacag cagccacctc cagagacctt tgacttcaaa atcaagtttg ctgagactaa
     3061 ggcccatgcc aaggcaattg agatggaatt gaggcagatg gaggtggccc aggccaatcg
     3121 acacatgtcc ctgctgacag ccttcatgcc tgacagcttc cttcggccag gtggggacca
     3181 tgactgcgtt ctggtgctgt tgctcatgcc tcgtctcatt tgcaaggcag agctgatccg
     3241 gaagcaggcc caggagaagt ttgaactaag tgagaactgt tcagagcggc ctgggctgcg
     3301 aggagctgct ggggagcaac tcagctttgc tgctggactg gtgtactcgc tgagcctgct
     3361 gcaggccacg ctacaccgct atgagcatgc cctctctcag tgcagtgtgg atgtgtataa
     3421 gaaagtgggc agcctgtacc ctgagatgag tgcccatgag cgctccttgg atttcctcat
     3481 tgaactgctg cacaaggatc agctggatga gactgtcaat gtggagcctc tcaccaaggc
     3541 catcaagtac tatcagcatc tgtacagcat ccaccttgcc gaacagcctg aggactgtac
     3601 tatgcagctg gctgaccaca ttaagttcac gcagagtgct ctggactgca tgagtgtgga
     3661 ggtaggacgg ctgcgtgcct tcttgcaggg tgggcaggag gctacagata ttgccctcct
     3721 gctccgggat ctggaaactt catgcagtga catccgccag ttctgcaaga agatccgaag
     3781 gcgaatgcca gggacagatg ctccttaaga cccagctttc ttgtacaaag ttggcattat
     3841 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3901 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     3961 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     4021 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     4081 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     4141 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     4201 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     4261 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     4321 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     4381 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     4441 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     4501 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     4561 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     4621 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     4681 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     4741 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     4801 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     4861 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     4921 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     4981 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5161 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
