LOCUS       sequence-203065-        9133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dLbCpf1-eBE in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, 
            Huang X, Yang L, Chen J
  TITLE     Base editing with a Cpf1-cytidine deaminase fusion.
  JOURNAL   Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102.
  PUBMED    29553573
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102."
COMMENT     Sequence Label: pCMV-dCpf1-eBE
FEATURES             Location/Qualifiers
     source          1..9133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             412..432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             433..1119
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             4858..4878
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4891..5139
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5152..5172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5179..5232
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5233..5481
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5482..5502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5503..5559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5560..5808
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5809..5829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5830..5883
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5884..6132
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6145..6165
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6188..6205)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6194..6418
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6489..6505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6489..6505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6502..6524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6513..6529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6537..6543,6544..6561,6562..6567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6582..6603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6720..6737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6891..7479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6971..6990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7650..8441,8442..8510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8273..8292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8511..8615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8694..8713)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8885..9133,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gcccaagaag
      421 aagaggaaag tcatgagctc agagactggc ccagtggctg tggaccccac attgagacgg
      481 cggatcgagc cccatgagtt tgaggtattc ttcgatccga gagagctccg caaggagacc
      541 tgcctgcttt acgaaattaa ttgggggggc cggcactcca tttggcgaca tacatcacag
      601 aacactaaca agcacgtcga agtcaacttc atcgagaagt tcacgacaga aagatatttc
      661 tgtccgaaca caaggtgcag cattacctgg tttctcagct ggagcccatg cggcgaatgt
      721 agtagggcca tcactgaatt cctgtcaagg tatccccacg tcactctgtt tatttacatc
      781 gcaaggctgt accaccacgc tgacccccgc aatcgacaag gcctgcggga tttgatctct
      841 tcaggtgtga ctatccaaat tatgactgag caggagtcag gatactgctg gagaaacttt
      901 gtgaattata gcccgagtaa tgaagcccac tggcctaggt atccccatct gtgggtacga
      961 ctgtacgttc ttgaactgta ctgcatcata ctgggcctgc ctccttgtct caacattctg
     1021 agaaggaagc agccacagct gacattcttt accatcgctc ttcagtcttg tcattaccag
     1081 cgactgcccc cacacattct ctgggccacc gggttgaaaa gcggcagcga gactcccggg
     1141 acctcagagt ccgccacacc cgaaagtatg tcaaagctgg agaaattcac caactgttat
     1201 agcctgtcta agaccctgcg cttcaaggca atcccagtgg gcaagacaca agagaacatt
     1261 gacaacaaac ggctcctggt ggaggatgag aagagggctg aagattacaa gggcgttaag
     1321 aagctgctgg ataggtacta tctgtcattc atcaacgatg tcctccacag tatcaagctg
     1381 aagaatctga acaattacat ttctctgttc cggaagaaga cacggaccga gaaggagaac
     1441 aaagagctgg agaatctgga gatcaacctg aggaaagaaa tagctaaggc tttcaaaggg
     1501 aacgagggtt acaagtccct gttcaagaaa gacattatcg agactattct gcctgagttc
     1561 ctggacgata aagatgagat cgccctcgtc aattccttca atgggtttac cacagccttt
     1621 accggcttct tcgacaatag agagaatatg ttctctgaag aggccaaatc cactagcatc
     1681 gcctttcgct gcataaacga gaacctgact aggtacatca gcaatatgga catctttgag
     1741 aaagtcgatg ccatattcga caaacatgag gtgcaggaga ttaaggagaa gatcctgaac
     1801 tcagattacg atgtcgaaga tttcttcgag ggagagttct tcaacttcgt gctcacacaa
     1861 gagggcattg atgtgtacaa tgcaatcatt ggagggttcg tgacagagag tggcgagaag
     1921 ataaagggcc tgaacgagta tatcaacctc tacaaccaga aaaccaagca gaaactgcct
     1981 aagttcaagc cactgtacaa acaagtgctc tcagataggg aaagcctgag cttctacggt
     2041 gaagggtata catcagatga agaagtgctc gaagtgttcc gcaacaccct caataagaac
     2101 agtgaaatct tctcttcaat caagaagctg gagaaactgt tcaagaattt cgatgagtac
     2161 tcctctgccg gaatctttgt gaagaatggc cctgcaatat ccactattag caaagacatc
     2221 tttggcgagt ggaacgttat cagggataag tggaatgccg agtacgatga tattcatctc
     2281 aagaagaaag ccgtggttac agagaaatac gaggatgata gacgcaagag ctttaagaag
     2341 attggtagct tctctctcga acagctgcag gagtacgccg acgctgacct gtcagtcgtg
     2401 gagaaactca aggagatcat aatccagaag gtggatgaaa tctacaaagt gtatggaagc
     2461 tctgagaaac tcttcgatgc agactttgtt ctggagaaga gtctgaagaa gaacgacgca
     2521 gtggttgcta tcatgaagga cctgctggat tctgttaagt ctttcgagaa ttacattaag
     2581 gcattctttg gtgaagggaa ggagacaaat agggacgaga gcttctatgg cgactttgtt
     2641 ctggcctacg acatcctcct caaggttgac cacatctatg acgctatacg gaattacgtt
     2701 acccagaagc cctatagcaa agacaagttc aagctgtatt tccagaatcc acagtttatg
     2761 ggtgggtggg ataaagacaa agaaacagat tacagggcca ctatcctgcg gtacggcagc
     2821 aaatactatc tggctatcat ggataagaag tacgccaaat gcctccagaa gatcgacaag
     2881 gacgacgtga acggtaacta cgagaagatc aattacaagc tcctgccagg acctaacaag
     2941 atgctgccca aggtgttctt ctccaagaaa tggatggcct actataaccc aagcgaggac
     3001 attcagaaga tatacaagaa tgggacattc aagaagggcg atatgttcaa cctcaacgac
     3061 tgccacaagc tgattgattt cttcaaggat agcatttctc gctatcccaa gtggtctaat
     3121 gcatacgatt tcaacttcag cgagactgag aagtacaaag acatcgctgg cttctaccgg
     3181 gaggtggaag agcaaggcta taaggtgtca ttcgaatccg cttctaagaa ggaagtggat
     3241 aagctcgtgg aagagggtaa gctgtacatg ttccagatat acaacaaaga cttcagcgat
     3301 aagagccacg gcactccaaa cctccatact atgtatttca agctgctgtt tgacgagaac
     3361 aaccacggac agattaggct gtcaggaggc gcagaactct tcatgcgcag agcttcactg
     3421 aagaaggagg aactcgttgt ccacccagcc aatagcccta tagccaataa gaatccagac
     3481 aatcctaaga aaaccactac tctgtcttac gatgtgtata aggataagag attctctgaa
     3541 gatcagtacg aactgcacat acccattgcc attaacaagt gccctaagaa catcttcaag
     3601 attaacacag aggttagagt gctcctgaaa cacgacgata acccttatgt tataggcatt
     3661 gctcgcggag agagaaacct gctgtacatc gtcgtggtgg acggcaaagg caacatcgtg
     3721 gaacagtaca gtctcaatga aatcattaac aatttcaacg gaatccgcat taagaccgac
     3781 taccattctc tcctcgacaa gaaggagaaa gaaaggttcg aagcaagaca gaattggaca
     3841 agtatagaga atatcaaaga actgaaggct gggtacatct ctcaggttgt gcacaagata
     3901 tgtgagctgg tggagaagta cgacgctgtt atcgccctcg cggacctgaa tagcggcttc
     3961 aagaactcca gggtgaaggt ggagaagcag gtgtatcaga agttcgagaa gatgctgatc
     4021 gacaagctca actatatggt ggacaagaaa tccaatcctt gcgctactgg tggagccctg
     4081 aagggctatc aaatcaccaa taagttcgaa tctttcaagt ctatgagcac ccagaatggc
     4141 ttcatcttct acatacccgc atggctgaca tccaagattg atccctctac cggatttgtt
     4201 aatctgctca agactaagta cacctctatt gctgactcaa agaagttcat atcatcattt
     4261 gaccgcatca tgtacgtgcc agaagaggac ctgttcgagt ttgccctgga ttacaagaat
     4321 ttctctcgga ctgacgccga ctacatcaag aagtggaagc tctactctta tggtaatcgg
     4381 attcgcatat tccgcaatcc caagaagaat aacgtgttcg attgggagga agtttgcctc
     4441 accagcgctt acaaggagct gttcaataag tatgggatta actaccagca gggcgacata
     4501 agagccctgc tgtgcgaaca atctgataag gcattctatt cctctttcat ggcactgatg
     4561 tcactgatgc tgcaaatgcg caattccatc accggaagaa cagacgtggc ctttctgatc
     4621 tctcctgtca agaactcaga tggcatcttc tacgattccc gcaactatga agcacaggag
     4681 aatgctatcc tgcctaagaa tgccgatgca aatggagcct ataacatcgc cagaaaggtc
     4741 ctctgggcca taggacaatt caagaaagct gaagatgaga agctggacaa ggtgaagatc
     4801 gccatttcaa acaaagagtg gctcgaatat gctcagacct cagtgaagca tggatcaccc
     4861 aagaagaaac ggaaagtgtc tggtggttct actaatctgt cagatattat tgaaaaggag
     4921 accggtaagc aactggttat ccaggaatcc atcctcatgc tcccagagga ggtggaagaa
     4981 gtcattggga acaagccgga aagcgatata ctcgtgcaca ccgcctacga cgagagcacc
     5041 gacgagaatg tcatgcttct gactagcgac gcccctgaat acaagccttg ggctctggtc
     5101 atacaggata gcaacggtga gaacaagatt aagatgctct ctggtggttc tcccaagaag
     5161 aagaggaaag tcgggcccga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     5221 aatcctggcc caactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt
     5281 atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg
     5341 gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt
     5401 ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt
     5461 gagaacaaga ttaagatgct ccccaagaag aagaggaaag tcgctactaa cttcagcctg
     5521 ctgaagcagg ctggagacgt ggaggagaac cctggaccta ccaacctgtc cgatatcatt
     5581 gagaaagaga ccggcaaaca gctggtgatc caggagagca tcctgatgct gcccgaagag
     5641 gtggaggaag tgatcggcaa caagcccgag tccgacatcc tggtgcacac agcctatgat
     5701 gaatccaccg acgagaacgt gatgctgctg acctccgatg ctcccgagta taaaccctgg
     5761 gcactggtga tccaggactc taatggagag aacaagatca agatgctgcc caagaagaag
     5821 aggaaagtcg aaggacgggg ctccctcctg acctgtggcg atgtggaaga gaaccccggc
     5881 cccacaaacc tcagtgacat tatcgagaag gaaacaggaa aacagctcgt cattcaagaa
     5941 tctattctta tgttgcctga ggaagtcgaa gaggttattg gcaataaacc tgaatctgat
     6001 attcttgtcc ataccgctta cgatgagtcc acagatgaaa atgttatgct gctcacatct
     6061 gacgcaccag agtacaaacc atgggcgctc gttattcaag attccaacgg cgaaaacaaa
     6121 atcaaaatgc tttctggtgg ttctcctaaa aaaaaaagaa aggtgtgagt ttaaacccgc
     6181 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     6241 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     6301 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     6361 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     6421 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
     6481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6541 atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
     6601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8641 gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc
     8701 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     8761 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     8821 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     8881 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     8941 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9001 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9061 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9121 catcaagtgt atc
//
