LOCUS       sequence-203068-        4331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' element for Gateway cloning, 5 single nucleotide mutations 
            compared to the reference sequence, but none of them change the 
            amino acidtruncation aa416-1033(end) 1854 bp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Taverna E, Mora-Bermudez F, Strzyz PJ, Florio M, Icha J, Haffner C, 
            Norden C, Wilsch-Brauninger M, Huttner WB
  TITLE     Non-canonical features of the Golgi apparatus in bipolar epithelial 
            neural stem cells.
  JOURNAL   Sci Rep. 2016 Feb 16;6:21206. doi: 10.1038/srep21206.
  PUBMED    26879757
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:21206. doi"; pages: " 
            10.1038/srep21206"
COMMENT     Sequence Label: p3E DN Kif5a
FEATURES             Location/Qualifiers
     source          1..4331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     354..376
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     368..385
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     369..385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(456..579)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2440..2535)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2572..2591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2573..2591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2596..2612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2596..2612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2714..2733)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2725..3534
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2792..2811)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3681..4269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4170..4189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc
      361 acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat
      421 tctacaggtc actaatacca tctaagtagt tggttcatag tgactgcata tgttgtgttt
      481 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      541 cattttacgt ttctcgttca actttcttgt acaaagtggc ttcctctatc gtggtgcgca
      601 tctcagagga agagcggcag aaatacgagg aagagatccg caaactctac cgccagctgg
      661 acgacaagga tgatgagatc aatctccagt gtcagctggt ggagaagctg aaggagcaga
      721 ttctggatca ggatgagctg ctggcctcca cccgcggcga cagtgataag gtacagacgg
      781 agctgggtcg tctgcagacg gagagcgaga gcgccaaggc ggaggtgcgg gaggtgctgc
      841 aggcgctgga ggagctggcc gtcaactacg accagaagag ccaggaggtg gaggagaaga
      901 gtctgcacaa caaacagctg gccgagcagc tcagccacaa gatggccaac ctgatggagc
      961 tggaggcgga gctagcgcag atgcaggaag tgagctcaca gcagaggaag cgcatcgctg
     1021 atgtgttgaa cggcctgatg aaggacctca gcgagttcag cagcatcgtc ggaaacggag
     1081 aactcaagct accggtggag atcagcggtg cgattgagga ggagttcacc gtggcacgtc
     1141 tctacatcag caagattaaa tctgaggtga agtcgatggt gaagcgctgc cggcagctgg
     1201 agaacatgca gctggagtgc catcgcaaga tggaggagac cagccgggag ctgtcgtcat
     1261 gccaactgct catctcacag catgaggcaa agatccgctc tctcacagaa tacatgcaga
     1321 acgtggagca gaagaagaga cagctggagg acaactatga tgctctcagt gaagaactct
     1381 acacactgca gaactctgag agtcatgtgg cagagctgga tggaggagag aaggcggaca
     1441 gagaggctga tgggaaaaag gccgctgttc gtccagctct gcatgcggag tctcgtcaga
     1501 cgcagctcag ccgcctgcgg gatgaaatta acgagaagca gagactcatc gacgagctca
     1561 cagataagaa ccaggctctg gagttggagc tggctcacgt gcgctccagt ttcagcaatc
     1621 tgaagctgca ggacgacagc aagagcgcct gtctggagca gatgtcgttt caacaggaga
     1681 gacacgagca gtccaaacag gatctcaagg gccttgagga gactgttgct cgggaactcc
     1741 agaccctcca caacctgcgc aagctgttcg ttcaagacct cacgactcgg gttaaaaaaa
     1801 gttcagaaat tggacctgat gatagtgggg ggtctaacac ccagaagcag aagatttcct
     1861 ttcttgagaa caacctggat cagcttacaa aggttcataa acagctggta cgtgataatg
     1921 cagatctccg ctgtgagctt ccgaagctgg agaaacgtct tcgttctacg gctgagcggg
     1981 ttcgggcgct ggagacagca ctgaaggacg ccaaacaggg cgcgatgaat gaccggcgcc
     2041 ggtaccagca ggaggtggag cgcatccggg acgccatgag gctgcgttac ccactgcgcc
     2101 gccccaacgc cgctcagatc gctaagccag tgagacccgg gcatcacgtc accgccacat
     2161 cgcccaccag cttctccgtc acacgcgcaa gcaaaccggc cacgtcttac agcaatgccc
     2221 gattcctgca ggacgagaag atggagaaca gccagaagac caacactgtt ggatacagca
     2281 ctgtgagatc catagacata ctgagctcct gtccgctcga cgtggagaac ggaaacagca
     2341 cagatattaa tgataacagg agtgatgttc actgcggcag catggtggac gatgcctctc
     2401 gagtgtacat catgcagcag gagactgcag ccagttaagc aactttatta tacaaagttg
     2461 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2521 ataaaatcat tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt
     2581 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2641 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2701 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2761 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2821 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2881 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2941 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3001 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3061 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3121 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3181 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3241 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3301 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3361 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3421 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3481 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3541 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3601 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4321 gctcacatgt t
//
