LOCUS       sequence-203089-       10021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast mating-based Split Ubiquitin Assay, bait vector for in vivo 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Obrdlik P, El-Bakkoury M, Hamacher T, Cappellaro C, Vilarino C, 
            Fleischer C, Ellerbrok H, Kamuzinzi R, Ledent V, Blaudez D, Sanders 
            D, Revuelta JL, Boles E, Andre B, Frommer WB
  TITLE     K+ channel interactions detected by a genetic system optimized for 
            systematic studies of membrane protein interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12242-7. Epub 2004 Aug
            6.
  PUBMED    15299147
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-08-17"; volume: "101(33)"; pages: 
            "12242-7. Epub 2004 Aug 6"
COMMENT     Sequence Label: pMetYCgate
FEATURES             Location/Qualifiers
     source          1..10021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    288..309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(324..329,330..347,348..354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    362..378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     367..389
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     386..402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     386..402
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        491..841
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     protein_bind    842..866
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     gene            953..2309
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        953..1296
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1297..2106
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1364..1383)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2112..2309
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    complement(2363..2387)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2599..2772
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2773..2946
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             join(2965..3216,3217..3570)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3508..3529
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3589..3822
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(3974..3993)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4018..4066
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(4368..4387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4369..4387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4394..4411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4394..4410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4403..4425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5167..6329
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      5492..6329
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(6691..7785)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7786..8191)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(8211..8229)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8297..8401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8402..8470,8471..9262)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8620..8639)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9433..10021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9922..9941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      241 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      301 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      361 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
      421 gaaattaacc ctcactaaag ggaacaaaag ctgggtaccg gatgcaaggg ttcgaatccc
      481 ttagctctca ttattttttg ctttttctct tgaggtcaca tgatcgcaaa atggcaaatg
      541 gcacgtgaag ctgtcgatat tggggaactg tggtggttgg caaatgacta attaagttag
      601 tcaaggcgcc atcctcatga aaactgtgta acataataac cgaagtgtcg aaaaggtggc
      661 accttgtcca attgaacacg ctcgatgaaa aaaataagat atatataagg ttaagtaaag
      721 cgtctgttag aaaggaagtt tttccttttt cttgctctct tgtcttttca tctactattt
      781 ccttcgtgta atacagggtc gtcagataca tagatacaat tctattaccc ccatccatac
      841 aacaagtttg tacaaaaaag caggctctcc aaccaccatg aattctatac gaagttatta
      901 ggtctagaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
      961 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
     1021 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
     1081 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
     1141 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
     1201 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
     1261 atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt
     1321 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     1381 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     1441 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     1501 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     1561 cctgatgatg catggttact caccactgcg atccccggca aaacagcatt ccaggtatta
     1621 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     1681 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     1741 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     1801 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg
     1861 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     1921 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     1981 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2041 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2101 ttctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
     2161 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
     2221 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
     2281 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
     2341 tgtcgaaaac gagctcccgg gtacccagct ttcttgtaca aagtggttgg tggtggcgga
     2401 tccggtggag gtggatcagg tatccctcca gatcaacaaa gattgatctt tgccggtaag
     2461 cagctagaag acggtagaac gctgtctgat tacaacattc agaaggagtc caccttacat
     2521 cttgtgctaa ggctaagagg tggtatgcac agatcagctt gcggccgcat ggcaggcctt
     2581 gcgcaacacg atgaagccgt ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat
     2641 gagatcttac atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta
     2701 aaagatgacc caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct
     2761 caggcgccga aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc
     2821 ttacatttac ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat
     2881 gacccaagcc aaagcgctaa ccttttagca gaagctaaaa agctaaatga tgctcaggcg
     2941 ccgaaagtag acgcgagcgg ccgcatgaaa gcgttaacgg ccaggcaaca agaggtgttt
     3001 gatctcatcc gtgatcacat cagccagaca ggtatgccgc cgacgcgtgc ggaaatcgcg
     3061 cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac atctgaaggc gctggcacgc
     3121 aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga tccgtctgtt gcaggaagag
     3181 gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg aaccacttct ggcgcaacag
     3241 catattgaag gtcattatca ggtcgaccct tccttattca agccgaatgc tgatttcctg
     3301 ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta tggatggtga cttgctggca
     3361 gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg tcgcacgtat tgatgacgag
     3421 gttaccgtta agcgcctgaa aaaacagggc aataaagtcg aactgttgcc agaaaatagc
     3481 gagtttaaac caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg
     3541 gttggggtta ttcgcaacgg cgactggctg gaattcccgg ggatctgggc ccccccgacc
     3601 gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat ggcgcatgcc
     3661 gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc gggtccggga
     3721 tttacccccc acgactccgc cccctacggc gctctggata tggccgactt cgagtttgag
     3781 cagatgttta ccgatgccct tggaattgac gagtacggtg ggtagggggc gcgaccggac
     3841 ccgcatcccc cgtctgggtt ttcccctccc gtccccggtt cgtatccaca ataaacacga
     3901 gcacatacat tacaaaaacc tgcggttgtc gtctgattat ttggtggatc cgggggagat
     3961 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4021 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
     4081 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     4141 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     4201 gccaacgtcg gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt
     4261 cccccatggg gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc
     4321 aggtccacga cccaagctgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt
     4381 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4441 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4501 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4561 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgct gggccattct
     4621 catgaagaat atcttgaatt tattgtcata ttactagttg gtgtggaagt cctaatatcg
     4681 gtgatcaata tagtggttga catgctggct agtcaacatt gagccttttg atcatgcaaa
     4741 tatattacgg tattttacaa tcaaatatca aacttaacta ttgactttat aacttattta
     4801 ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa aacagtacta gcttttaact
     4861 tgtatcctag gttatctatg ctgtctcacc atagagaata ttacctattt cagaatgtat
     4921 gtccatgatt cgccgggtaa atacatataa tacacaaatc tggcttaata aagtctataa
     4981 tatatctcat aaagaagtgc taaattggct agtgctatat atttttaaga aaatttcttt
     5041 tgactaagtc catatcgact ttgtaaaagt tcacattagc atacatatat tacacgagcc
     5101 agaaatagta acttttgcct aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca
     5161 catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac
     5221 ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa
     5281 aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc
     5341 tttcatagct taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat
     5401 tcttccttcc ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagttc
     5461 gatgataagc tgtcaaacat gagaattaat tccacatgtt aaaatagtga aggagcatgt
     5521 tcggcacaca gtggaccgaa cgtggggtaa gtgcactagg gtccggttaa acggatctcg
     5581 cattgatgag gcaacgctaa ttatcaacat atagattgtt atctatctgc atgaacacga
     5641 aatctttact tgacgacttg aggctgatgg tgtttatgca aagaaaccac tgtgtttaat
     5701 atgtgtcact gtttgatatt actgtcagcg tagaagataa tagtaaaagc ggttaataag
     5761 tgtatttgag ataagtgtga taaagttttt acagcgaaaa gacgataaat acaagaaaat
     5821 gattacgagg atacggagag aggtatgtac atgtgtattt atatactaag ctgccggcgg
     5881 ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt
     5941 gaacattctc ttcaacaagt ttgattccat tgcggtgaaa tggtaaaagt caaccccctg
     6001 cgatgtatat tttcctgtac aatcaatcaa aaagccaaat gatttagcat tatctttaca
     6061 tcttgttatt ttacagattt tatgtttaga tcttttatgc ttgcttttca aaaggcttgc
     6121 aggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta tttcttagca
     6181 tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg
     6241 cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc
     6301 agtccaccag ctaacataaa atgtaagctc tcggggctct cttgccttcc aacccagtca
     6361 gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga
     6421 ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat
     6481 acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct
     6541 ccatgcagtt ggacgatcga tgataagctg tcaaacatga gaattaattc taccctatga
     6601 acatattcca ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat
     6661 gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc
     6721 gacagcatca ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc
     6781 tgcatccaaa acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa
     6841 tgacaatttc aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt
     6901 tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc
     6961 tggcaaagag gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc
     7021 ttcatcggag atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt
     7081 tgggttctta actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt
     7141 aggaaattcg ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa
     7201 aacattagct ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa
     7261 agcggccatt cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc
     7321 gacaccatca ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct
     7381 gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag
     7441 agagtcggat gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat
     7501 ttttagtaaa ccttgttcag gtctaacact acctgtaccc catttaggac cacccacagc
     7561 acctaacaaa acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac
     7621 acctgtagca tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt
     7681 ggaacgaaca tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc
     7741 aacgtggtca cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc
     7801 ttgaaatata tatatatatt gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg
     7861 attgctaacc acctattgga aaaaacaata ggtccttaaa taatattgtc aacttcaagt
     7921 attgtgatgc aagcatttag tcatgaacgc ttctctattc tatatgaaaa gccggttccg
     7981 gcgctctcac ctttcctttt tctcccaatt tttcagttga aaaaggtata tgcgtcaggc
     8041 gacctctgaa attaacaaaa aatttccagt catcgaattt gattctgtgc gatagcgccc
     8101 ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg ctaagagatt cgaactcttg
     8161 catcttacga tacctgagta ttcccacagt taattcttga agacgaaagg gcctcgtgat
     8221 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     8281 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9001 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9301 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9361 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10021 a
//
