LOCUS       sequence-203111-       17875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also 
            expresses Cas9 from AtUBQ10 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17875)
  AUTHORS   Curtin SJ, Xiong Y, Michno JM, Campbell BW, Stec AO, Cermak T, 
            Starker C, Voytas DF, Eamens AL, Stupar RM
  TITLE     CRISPR/Cas9 and TALENs generate heritable mutations for genes 
            involved in small RNA processing of Glycine max and Medicago 
            truncatula.
  JOURNAL   Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
  PUBMED    29087011
REFERENCE   2  (bases 1 to 17875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857."
COMMENT     Sequence Label: pSC44
FEATURES             Location/Qualifiers
     source          1..17875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1220..1987
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             2179..2973
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             3272..4420
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    6016..6040
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6287..6308)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6499..6518
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6658..6674
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6687..6939)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7083..7634)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7655..8000)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7692..7712)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     8536..8552
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9235..9259
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9281..9328
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             9335..9355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(9380..10816,11006..13669)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          10817..11005
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             13670..13717
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      13728..13980
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    14025..14045
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        14527..14602
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(14614..14638)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14657..14681)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      14745..14997
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(15015..15031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15015..15031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    15297..15321
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15786..16436
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(16624..16641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(16875..16894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            17117..17226
                     /note="incP origin of transfer"
     CDS             17259..17630
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      join(17757..17875,1..597)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
       61 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
      121 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
      181 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
      241 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
      301 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
      361 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
      421 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
      481 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
      541 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
      601 tggcagtcct tgccattgcc gggatcgggg cagtaacggg atgggcgatc agcccgagcg
      661 cgacgcccgg aagcattgac gtgccgcagg tgctggcatc gacattcagc gaccaggtgc
      721 cgggcagtga gggcggcggc ctgggtggcg gcctgccctt cacttcggcc gtcggggcat
      781 tcacggactt catggcgggg ccggcaattt ttaccttggg cattcttggc atagtggtcg
      841 cgggtgccgt gctcgtgttc gggggtgcga taaacccagc gaaccatttg aggtgatagg
      901 taagattata ccgaggtatg aaaacgagaa ttggaccttt acagaattac tctatgaagc
      961 gccatattta aaaagctacc aagacgaaga ggatgaagag gatgaggagg cagattgcct
     1021 tgaatatatt gacaatactg ataagataat atatctttta tatagaagat atcgccgtat
     1081 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     1141 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     1201 gtaaattcta tcataattgg gtaatgactc caacttattg atagtgtttt atgttcagat
     1261 aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc gacttccgtc
     1321 ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc
     1381 gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg
     1441 tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc agcgtcgcca
     1501 tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca tacgcgtaaa
     1561 acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc atttccgcgc
     1621 agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc ctgagttttt
     1681 taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg cccggcatcc
     1741 aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag aagcggtgta
     1801 agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct gatgtccggc
     1861 ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac agctgataga
     1921 aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag
     1981 catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
     2041 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
     2101 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
     2161 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     2221 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     2281 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     2341 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     2401 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     2461 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     2521 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     2581 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     2641 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
     2701 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     2761 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     2821 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     2881 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     2941 aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca acgatgccgg
     3001 cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc aggccgaggc
     3061 ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga ttcggaatac
     3121 caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg ataaggtgga
     3181 ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca ttgccccggc
     3241 gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc ggaaggcata
     3301 caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca tcgcaagccg
     3361 caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc
     3421 tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc ccgcgccatc
     3481 ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat
     3541 gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg gcgaggacct
     3601 ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga agcagcagat
     3661 caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat
     3721 gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga
     3781 ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca cctacaccgg
     3841 cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg agtacgcgaa
     3901 gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc aggacctggg
     3961 ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc
     4021 gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca
     4081 ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga
     4141 ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg agaagtaccg
     4201 caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc gggagccgta
     4261 cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa
     4321 gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc tggtggaaca
     4381 cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg ggtcagttcc
     4441 ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg gcactgctcg
     4501 acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga actgccgata
     4561 aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct tggagcggcc
     4621 gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc agagatgttc
     4681 gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga acggttgcga
     4741 gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc ggtcttcaaa
     4801 caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt cgtggagccc
     4861 gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc gggtttattg
     4921 ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat cttcatcctc
     4981 ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta ccgcctgccg
     5041 ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt catctctgcc
     5101 gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg cggaactgcg
     5161 ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg cgtcgcagcg
     5221 ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa gtggcaacct
     5281 cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact tctgctcgtt
     5341 ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa tgttctcggc
     5401 ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt ccgggggatc
     5461 tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagccccag atctggggtc
     5521 gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac ctgtaccatt
     5581 cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag aaatagcgcc
     5641 actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat agttctcaag
     5701 atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat tcggatctct
     5761 gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac aatcaacatg
     5821 ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa
     5881 tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc
     5941 ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag
     6001 ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact
     6061 taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc accagtgaga
     6121 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     6181 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat
     6241 cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa
     6301 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6361 cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa
     6421 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6481 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc aggctgcgca
     6541 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6601 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6661 aaacgacggc cagtgaatta attcccgatc tagtaacata gatgacaccg cgcgcgataa
     6721 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     6781 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     6841 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     6901 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctcggtac
     6961 cccctcctgg gcacggtcgt acgcgatccg ccgcggttcc acctcaccat tgcgggtatg
     7021 ccagttgagt tcgatgagtt cccgtaccag ctccaactcg ggcgatgcca cgcgcactcc
     7081 gttcagatct cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc
     7141 tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg
     7201 cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg
     7261 acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc
     7321 gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc
     7381 cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg
     7441 tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac
     7501 tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg
     7561 acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt
     7621 cgttctgggc tcatggatcc tctagagtcc cccgtgttct ctccaaatga aatgaacttc
     7681 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     7741 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     7801 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttcaa
     7861 cgatggcctt tcctttatcg caatgatggc atttgtagga gccaccttcc ttttccacta
     7921 tcttcacaat aaagtgacag atagctgggc aatggaatcc gaggaggttt ccggatatta
     7981 ccctttgttg aaaagtctca attgcccttt ggtcttctga gactgtatct ttgatatttt
     8041 tggagtagac aagtgtgtcg tgctccacca tgttgacgaa gattttcttc ttgtcattga
     8101 gtcgtaagag actctgtatg aactgttcgc cagtctttac ggcgagttct gttaggtcct
     8161 ctatttgaat ctttgactcc atggcctttg attcagtggg aactaccttt ttagagactc
     8221 caatctctat tacttgcctt ggtttgtgaa gcaagccttg aatcgtccat actggaatag
     8281 tacttctgat cttgagaaat atatctttct ctgtgttctt gatgcagtta gtcctgaatc
     8341 ttttgactgc atctttaacc ttcttgggaa ggtatttgat ttcctggaga ttattgctcg
     8401 ggtagatcgt cttgatgaga cctgctgcgt aagcctctct aaccatctgt gggttagcat
     8461 tctttctgaa attgaaaagg ctaatctggg gacctgcagg ggcaataccg ccagggtttt
     8521 cccagtcacg acgttgtaaa acgacggcca gtgccaagct ttcgacgagt cagtaataaa
     8581 cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa
     8641 tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac
     8701 gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga
     8761 cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc
     8821 ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct
     8881 accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc
     8941 gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga
     9001 cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata
     9061 tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt
     9121 tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtaga ttaatctgag
     9181 tttttctgat taacagggta ccgggccccc cctcgaggcg cgccaagcta tcaaacaagt
     9241 ttgtacaaaa aagcaggctc cgaattcgcc cttcaccatg gattacaagg atgatgatga
     9301 taaggattac aaggatgatg atgataagat ggctccaaag aagaagagaa aggttggaat
     9361 ccacggagtt ccagctgctg ataagaagta ctctatcgga cttgacatcg gaaccaactc
     9421 tgttggatgg gctgttatca ccgatgagta caaggttcca tctaagaagt tcaaggttct
     9481 tggaaacacc gatagacact ctatcaagaa gaaccttatc ggtgctcttc ttttcgattc
     9541 tggagagacc gctgaggcta ccagattgaa gagaaccgct agaagaagat acaccagaag
     9601 aaagaacaga atctgctacc ttcaggaaat cttctctaac gagatggcta aggttgatga
     9661 ttctttcttc cacagacttg aggagtcttt ccttgttgag gaggataaga agcacgagag
     9721 acacccaatc ttcggaaaca tcgttgatga ggttgcttac cacgagaagt acccaaccat
     9781 ctaccacctt agaaagaagt tggttgattc taccgataag gctgatctta gacttatcta
     9841 ccttgctctt gctcacatga tcaagttcag aggacacttc cttatcgagg gagaccttaa
     9901 cccagataac tctgatgttg ataagttgtt catccagctt gttcagacct acaaccagct
     9961 tttcgaggag aacccaatca acgcttctgg agttgatgct aaggctatcc tttctgctag
    10021 actttctaag tctcgtagac ttgagaacct tatcgctcag cttccaggag agaagaagaa
    10081 cggacttttc ggaaacctta tcgctctttc tcttggactt accccaaact tcaagtctaa
    10141 cttcgatctt gctgaggatg ctaagttgca gctttctaag gatacctacg atgatgatct
    10201 tgataacctt cttgctcaga tcggagatca gtacgctgat cttttccttg ctgctaagaa
    10261 cctttctgat gctatccttc tttctgacat ccttagagtt aacaccgaga tcaccaaggc
    10321 tccactttct gcttctatga tcaagagata cgatgagcac caccaggatc ttaccctttt
    10381 gaaggctctt gttagacagc agcttccaga gaagtacaag gaaatcttct tcgatcagtc
    10441 taagaacgga tacgctggat acatcgatgg aggagcttct caggaggagt tctacaagtt
    10501 catcaagcca atccttgaga agatggatgg aaccgaggag cttcttgtta agttgaacag
    10561 agaggatctt cttagaaagc agagaacctt cgataacgga tctatcccac accagatcca
    10621 ccttggagag cttcacgcta tccttcgtag acaggaggat ttctacccat tcttgaagga
    10681 taacagagag aagatcgaga agatccttac cttcagaatc ccatactacg ttggaccact
    10741 tgctagagga aactctcgtt tcgcttggat gaccagaaag tctgaggaga ccatcacccc
    10801 ttggaacttc gaggaggtaa gtttctgctt ctacctttga tatatatata ataattatca
    10861 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
    10921 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
    10981 atgaccaaaa tttgttgatg tgcaggttgt tgataaggga gcttctgctc agtctttcat
    11041 cgagagaatg accaacttcg ataagaacct tccaaacgag aaggttcttc caaagcactc
    11101 tcttctttac gagtacttca ccgtttacaa cgagcttacc aaggttaagt acgttaccga
    11161 gggaatgaga aagccagctt tcctttctgg agagcagaag aaggctatcg ttgatcttct
    11221 tttcaagacc aacagaaagg ttaccgttaa gcagttgaag gaggattact tcaagaagat
    11281 cgagtgcttc gattctgttg aaatctctgg agttgaggat agattcaacg cttctcttgg
    11341 aacctaccac gatcttttga agatcatcaa ggataaggat ttccttgata acgaggagaa
    11401 cgaggacatc cttgaggaca tcgttcttac ccttaccctt ttcgaggata gagagatgat
    11461 cgaggagaga ctcaagacct acgctcacct tttcgatgat aaggttatga agcagttgaa
    11521 gagaagaaga tacaccggat ggggtagact ttctcgtaag ttgatcaacg gaatcagaga
    11581 taagcagtct ggaaagacca tccttgattt cttgaagtct gatggattcg ctaacagaaa
    11641 cttcatgcag cttatccacg atgattctct taccttcaag gaggacatcc agaaggctca
    11701 ggtttctgga cagggagatt ctcttcacga gcacatcgct aaccttgctg gatctccagc
    11761 tatcaagaag ggaatccttc agaccgttaa ggttgttgat gagcttgtta aggttatggg
    11821 tagacacaag ccagagaaca tcgttatcga gatggctaga gagaaccaga ccacccagaa
    11881 gggacagaag aactctcgtg agagaatgaa gagaatcgag gagggaatca aggagcttgg
    11941 atctcaaatc ttgaaggagc acccagttga gaacacccag cttcagaacg agaagttgta
    12001 cctttactac cttcagaacg gaagagatat gtacgttgat caggagcttg acatcaacag
    12061 actttctgat tacgatgttg atcacatcgt tccacagtct ttcttgaagg atgattctat
    12121 cgataacaag gttcttaccc gttctgataa gaacagagga aagtctgata acgttccatc
    12181 tgaggaggtt gttaagaaga tgaagaacta ctggagacag cttcttaacg ctaagttgat
    12241 cacccagaga aagttcgata accttaccaa ggctgagaga ggaggacttt ctgagcttga
    12301 taaggctgga ttcatcaaga gacagcttgt tgagaccaga cagatcacca agcacgttgc
    12361 tcagatcctt gattctcgta tgaacaccaa gtacgatgag aacgataagt tgatcagaga
    12421 ggttaaggtt atcaccttga agtctaagtt ggtttctgat ttcagaaagg atttccagtt
    12481 ctacaaggtt agagagatca acaactacca ccacgctcac gatgcttacc ttaacgctgt
    12541 tgttggaacc gctcttatca agaagtaccc aaagttggag tctgagttcg tttacggaga
    12601 ttacaaggtt tacgatgtta gaaagatgat cgctaagtct gagcaggaga tcggaaaggc
    12661 taccgctaag tacttcttct actctaacat catgaacttc ttcaagaccg agatcaccct
    12721 tgctaacgga gagatcagaa agagaccact tatcgagacc aacggagaga ccggagagat
    12781 cgtttgggat aagggaagag atttcgctac cgttagaaag gttctttcta tgccacaggt
    12841 taacatcgtt aagaaaaccg aggttcagac cggaggattc tctaaggagt ctatccttcc
    12901 aaagagaaac tctgataagt tgatcgctag aaagaaggat tgggacccaa agaagtacgg
    12961 aggattcgat tctccaaccg ttgcttactc tgttcttgtt gttgctaagg ttgagaaggg
    13021 aaagtctaag aagttgaagt ctgttaagga gcttcttgga atcaccatca tggagcgttc
    13081 ttctttcgag aagaacccaa tcgatttcct tgaggctaag ggatacaagg aggttaagaa
    13141 ggatcttatc atcaagttgc caaagtactc tcttttcgag cttgagaacg gaagaaagag
    13201 aatgcttgct tctgctggag agcttcagaa gggaaacgag cttgctcttc catctaagta
    13261 cgttaacttc ctttaccttg cttctcacta cgagaagttg aagggatctc cagaggataa
    13321 cgagcagaag cagcttttcg ttgagcagca caagcactac cttgatgaga tcatcgagca
    13381 aatctctgag ttctctaaga gagttatcct tgctgatgct aaccttgata aggttctttc
    13441 tgcttacaac aagcacagag ataagccaat cagagagcag gctgagaaca tcatccacct
    13501 tttcaccctt accaaccttg gtgctccagc tgctttcaag tacttcgata ccaccatcga
    13561 tagaaaaaga tacacctcta ccaaggaggt tcttgatgct acccttatcc accagtctat
    13621 caccggactt tacgagacca gaatcgatct ttctcagctt ggaggagata agagaccagc
    13681 tgctaccaag aaggctggac aggctaagaa gaagaagtga gacgtccgat cgttcaaaca
    13741 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
    13801 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
    13861 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
    13921 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
    13981 gggaattgat cccccctcga cagcttccgg aaagggcgaa ttcgcaactt tgtatacaaa
    14041 agttgccgag ctcgcggccg ccttcgttga acaacggaaa ctcgacttgc cttccgcaca
    14101 atacatcatt tcttcttagc tttttttctt cttcttcgtt catacagttt ttttttgttt
    14161 atcagcttac attttcttga accgtagctt tcgttttctt ctttttaact ttccattcgg
    14221 agtttttgta tcttgtttca tagtttgtcc caggattaga atgattaggc atcgaacctt
    14281 caagaatttg attgaataaa acatcttcat tcttaagata tgaagataat cttcaaaagg
    14341 cccctgggaa tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt
    14401 tcttatatag gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa
    14461 gaaaacgaag ctgagtttat atacagctag agtcgaagta gtgattgaaa ttgaatgata
    14521 tctgtggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
    14581 aaaagtggca ccgagtcggt gctttttttc tagacccagc tttcttgtac aaagttggca
    14641 ttactgcagg tcgacgaccc agctttcttg tacaaagtgg ttcgataatt cttaattaac
    14701 tagttctaga gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt tcaaacattt
    14761 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
    14821 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
    14881 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
    14941 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
    15001 aattcgtaat catggtcata gctgtttcct gcctagtcgg agcggccgcg tccgtctagc
    15061 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
    15121 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
    15181 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
    15241 ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    15301 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    15361 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
    15421 tccccagatc tggcgccggc cagcgagacg agcaagattg gccgccgccc gaaacgatcc
    15481 gacagcgcgc ccagcacagg tgcgcaggca aattgcacca acgcatacag cgccagcaga
    15541 atgccatagt gggcggtgac gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc
    15601 accggcataa tcaggccgat gccgacagcg tcgagcgcga cagtgctcag aattacgatc
    15661 aggggtatgt tgggtttcac gtctggcctc cggaccagcc tccgctggtc cgattgaacg
    15721 cgcggattct ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt
    15781 caagcatgac aaagttgcag ccgaatacag tgatccgtgc cgccctggac ctgttgaacg
    15841 aggtcggcgt agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc
    15901 cggcgcttta ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca
    15961 tgctggcgga gaatcatacg cattcggtgc cgagagccga cgacgactgg cgctcatttc
    16021 tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca
    16081 tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct
    16141 tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa tgcgctgatg acaatcagct
    16201 acttcactgt tggggccgtg cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg
    16261 gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg
    16321 acgaagccgg tccggacgca gcgttcgagc agggactcgc ggtgattgtc gatggattgg
    16381 cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag aaagggtgac gattgatcag
    16441 gaccgctgcc ggagcgcaac ccactcacta cagcagagcc atgtagacaa catcccctcc
    16501 ccctttccac cgcgtcagac gcccgtagca gcccgctacg ggctttttca tgccctgccc
    16561 tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg gccttctggc gctcttccgc
    16621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    16681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    16741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    16801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    16861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    16921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    16981 gcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
    17041 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
    17101 tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct
    17161 tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct
    17221 ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa
    17281 ccaggaaggg cagcccacct atcaaggtgt actgccttcc agacgaacga agagcgattg
    17341 aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta cctgctggcc gtcggccagg
    17401 gctacaaaat cacgggcgtc gtggactatg agcacgtccg cgagctggcc cgcatcaatg
    17461 gcgacctggg ccgcctgggc ggcctgctga aactctggct caccgacgac ccgcgcacgg
    17521 cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa gatcgaagag aagcaggacg
    17581 agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc agagccatga cttttttagc
    17641 cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg tccccatgcg ctccatcaag
    17701 aagagcgact tcgcggagct ggtgaagtac atcaccgacg agcaaggcaa gaccgagcgc
    17761 ctttccgacg ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc
    17821 tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc ggccg
//
