LOCUS       sequence-20313-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  TITLE     Buchler Lab plasmids
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p406GALS
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     387..410
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     431..447
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     431..447
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(481..497)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(482..498)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      500..747
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(516..534)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(764..783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(765..783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctcggaaga acttccttcc cttccgtgcg tcctcgtctt tcaccggtcg cgtttcctga
       61 aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt
      121 ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt caaatgaacg
      181 aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt
      241 aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg caaaaactgc
      301 ataaccactt taactaatac tttcaacatt ttcggtttgt attacttctt attcaaatgt
      361 aataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa
      421 aaccccggat tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat
      481 cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc acgccctccc
      541 cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt
      601 tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt
      661 tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt
      721 ttgggacgct cgaaggcttt aatttgcggc cggtacccat tcgccctata gtgagtcgta
      781 ttacgcgcgc tcac
//
