LOCUS       sequence-203136-         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDKI
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
       61 tcacattatg catcaaaaaa accaggtaat gaacctgggt ccgaactcta aactgctgaa
      121 agaatacaaa tcccagctga tcgaactgaa catcgaacag ttcgaagcag gtatcggtct
      181 gatcctgggt gatgcttaca tccgttctcg tgatgaaggt aaaacctact gtatgcagtt
      241 cgagtggaaa aacaaagcat acatggacca cgtatgtctg ctgtacgatc agtgggtact
      301 gtccccgccg cacaaaaaag aacgtgttaa ccacctgggt aacctggtaa tcacctgggg
      361 cgcccagact ttcaaacacc aagctttcaa caaactggct aacctgttca tcgttaacaa
      421 caaaaaaacc atcccgaaca acctggttga aaactacctg accccgatgt ctctggcata
      481 ctggttcatg gatgatggtg gtaaatggga ttacaacaaa aactctacca acaaatcgat
      541 cgtactgaac acccagtctt tcactttcga agaagtagaa tacctggtta agggtctgcg
      601 taacaaattc caactgaact gttacgtaaa aatcaacaaa aacaaaccga tcatctacat
      661 cgattctatg tcttacctga tcttctacaa cctgatcaaa ccgtacctga tcccgcagat
      721 gatgtacaaa ctgccgaaca ctatctcctc cgaaactttc ctgaaataat aa
//
