LOCUS       sequence-203148-       19621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 
            (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19621)
  AUTHORS   Curtin SJ, Xiong Y, Michno JM, Campbell BW, Stec AO, Cermak T, 
            Starker C, Voytas DF, Eamens AL, Stupar RM
  TITLE     CRISPR/Cas9 and TALENs generate heritable mutations for genes 
            involved in small RNA processing of Glycine max and Medicago 
            truncatula.
  JOURNAL   Plant Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857.
  PUBMED    29087011
REFERENCE   2  (bases 1 to 19621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2017 Oct 31. doi: 10.1111/pbi.12857."
COMMENT     Sequence Label: pSC51
FEATURES             Location/Qualifiers
     source          1..19621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(118..489)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            522..631
                     /note="incP origin of transfer"
     primer_bind     854..873
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1107..1124
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1312..1962)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2427..2451
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    3659..3683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3705..3752
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             3759..3779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3804..5240,5430..8093)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          5241..5429
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             8094..8141
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8152..8404
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8449..8469
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_RNA        8824..8899
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(8911..8935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        9413..9488
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9500..9524)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9543..9567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        10045..10120
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(10132..10156)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10175..10199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10237..10944
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        11494..11839
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11782..11802
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11860..12411
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      12555..12807
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12820..12837)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12820..12836)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12829..12851)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12976..12995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13186..13207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    13454..13478
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(15074..16222)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(16521..17315)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  17507..18274
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      18967..19602
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc
       61 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
      121 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
      181 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
      241 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
      301 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
      361 cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
      421 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
      481 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
      541 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
      601 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
      661 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
      721 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgcc acgcttcccg
      781 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      841 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      901 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      961 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1021 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     1081 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     1141 agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga
     1201 aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg
     1261 tctacatggc tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc
     1321 accctttctc ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac
     1381 aatcaccgcg agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat
     1441 cgcggcccgc aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc
     1501 atcgctgtcg ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat
     1561 cagcgcattg acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc
     1621 gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc
     1681 gcggtaggcg agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca
     1741 gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag
     1801 tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc
     1861 caaccgttcc gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc
     1921 cagggcggca cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact
     1981 gataaacata atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac
     2041 cagcggaggc tggtccggag gccagacgtg aaacccaaca tacccctgat cgtaattctg
     2101 agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc
     2161 ctgcgcgatc tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg
     2221 tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg
     2281 cggcggccaa tcttgctcgt ctcgctggcc ggcgccagat ctggggaacc ctgtggttgg
     2341 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
     2401 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
     2461 agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac
     2521 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     2581 caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta agcacatacg
     2641 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agacggacgc
     2701 ggccgcgggc ccaatataac aacgacgtcg taacagataa agcgaagctt gaaggtgcat
     2761 gtgactccgt caagattacg aaaccgccaa ctaccacgca aattgcaatt ctcaatttcc
     2821 tagaaggact ctccgaaaat gcatccaata ccaaatatta cccgtgtcat aggcaccaag
     2881 tgacaccata catgaacacg cgtcacaata tgactggaga agggttccac accttatgct
     2941 ataaaacgcc ccacacccct cctccttcct tcgcagttca attccaatat attccattct
     3001 ctctgtgtat ttccctacct ctcccttcaa ggttagtcga tttcttctgt ttttcttctt
     3061 cgttctttcc atgaattgtg tatgttcttt gatcaatacg atgttgattt gattgtgttt
     3121 tgtttggttt catcgatctt caattttcat aatcagattc agcttttatt atctttacaa
     3181 caacgtcctt aatttgatga ttctttaatc gtagatttgc tctaattaga gctttttcat
     3241 gtcagatccc tttacaacaa gccttaattg ttgattcatt aatcgtagat tagggctttt
     3301 ttcattgatt acttcagatc cgttaaacgt aaccatagat cagggctttt tcatgaatta
     3361 cttcagatcc gttaaacaac agccttattt tttatacttc tgtggttttt caagaaattg
     3421 ttcagatccg ttgacaaaaa gccttattcg ttgattctat atcgtttttc gagagatatt
     3481 gctcagatct gttagcaact gccttgtttg ttgattctat tgccgtggat tagggttttt
     3541 tttcacgaga ttgcttcaga tccgtactta agattacgta atggattttg attctgattt
     3601 atctgtgatt gttgactcga caggtacctt caaacctcga ggcgcgccaa gctatcaaac
     3661 aagtttgtac aaaaaagcag gctccgaatt cgcccttcac catggattac aaggatgatg
     3721 atgataagga ttacaaggat gatgatgata agatggctcc aaagaagaag agaaaggttg
     3781 gaatccacgg agttccagct gctgataaga agtactctat cggacttgac atcggaacca
     3841 actctgttgg atgggctgtt atcaccgatg agtacaaggt tccatctaag aagttcaagg
     3901 ttcttggaaa caccgataga cactctatca agaagaacct tatcggtgct cttcttttcg
     3961 attctggaga gaccgctgag gctaccagat tgaagagaac cgctagaaga agatacacca
     4021 gaagaaagaa cagaatctgc taccttcagg aaatcttctc taacgagatg gctaaggttg
     4081 atgattcttt cttccacaga cttgaggagt ctttccttgt tgaggaggat aagaagcacg
     4141 agagacaccc aatcttcgga aacatcgttg atgaggttgc ttaccacgag aagtacccaa
     4201 ccatctacca ccttagaaag aagttggttg attctaccga taaggctgat cttagactta
     4261 tctaccttgc tcttgctcac atgatcaagt tcagaggaca cttccttatc gagggagacc
     4321 ttaacccaga taactctgat gttgataagt tgttcatcca gcttgttcag acctacaacc
     4381 agcttttcga ggagaaccca atcaacgctt ctggagttga tgctaaggct atcctttctg
     4441 ctagactttc taagtctcgt agacttgaga accttatcgc tcagcttcca ggagagaaga
     4501 agaacggact tttcggaaac cttatcgctc tttctcttgg acttacccca aacttcaagt
     4561 ctaacttcga tcttgctgag gatgctaagt tgcagctttc taaggatacc tacgatgatg
     4621 atcttgataa ccttcttgct cagatcggag atcagtacgc tgatcttttc cttgctgcta
     4681 agaacctttc tgatgctatc cttctttctg acatccttag agttaacacc gagatcacca
     4741 aggctccact ttctgcttct atgatcaaga gatacgatga gcaccaccag gatcttaccc
     4801 ttttgaaggc tcttgttaga cagcagcttc cagagaagta caaggaaatc ttcttcgatc
     4861 agtctaagaa cggatacgct ggatacatcg atggaggagc ttctcaggag gagttctaca
     4921 agttcatcaa gccaatcctt gagaagatgg atggaaccga ggagcttctt gttaagttga
     4981 acagagagga tcttcttaga aagcagagaa ccttcgataa cggatctatc ccacaccaga
     5041 tccaccttgg agagcttcac gctatccttc gtagacagga ggatttctac ccattcttga
     5101 aggataacag agagaagatc gagaagatcc ttaccttcag aatcccatac tacgttggac
     5161 cacttgctag aggaaactct cgtttcgctt ggatgaccag aaagtctgag gagaccatca
     5221 ccccttggaa cttcgaggag gtaagtttct gcttctacct ttgatatata tataataatt
     5281 atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa agaatgtagt
     5341 atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat aacttttcta
     5401 atatatgacc aaaatttgtt gatgtgcagg ttgttgataa gggagcttct gctcagtctt
     5461 tcatcgagag aatgaccaac ttcgataaga accttccaaa cgagaaggtt cttccaaagc
     5521 actctcttct ttacgagtac ttcaccgttt acaacgagct taccaaggtt aagtacgtta
     5581 ccgagggaat gagaaagcca gctttccttt ctggagagca gaagaaggct atcgttgatc
     5641 ttcttttcaa gaccaacaga aaggttaccg ttaagcagtt gaaggaggat tacttcaaga
     5701 agatcgagtg cttcgattct gttgaaatct ctggagttga ggatagattc aacgcttctc
     5761 ttggaaccta ccacgatctt ttgaagatca tcaaggataa ggatttcctt gataacgagg
     5821 agaacgagga catccttgag gacatcgttc ttacccttac ccttttcgag gatagagaga
     5881 tgatcgagga gagactcaag acctacgctc accttttcga tgataaggtt atgaagcagt
     5941 tgaagagaag aagatacacc ggatggggta gactttctcg taagttgatc aacggaatca
     6001 gagataagca gtctggaaag accatccttg atttcttgaa gtctgatgga ttcgctaaca
     6061 gaaacttcat gcagcttatc cacgatgatt ctcttacctt caaggaggac atccagaagg
     6121 ctcaggtttc tggacaggga gattctcttc acgagcacat cgctaacctt gctggatctc
     6181 cagctatcaa gaagggaatc cttcagaccg ttaaggttgt tgatgagctt gttaaggtta
     6241 tgggtagaca caagccagag aacatcgtta tcgagatggc tagagagaac cagaccaccc
     6301 agaagggaca gaagaactct cgtgagagaa tgaagagaat cgaggaggga atcaaggagc
     6361 ttggatctca aatcttgaag gagcacccag ttgagaacac ccagcttcag aacgagaagt
     6421 tgtaccttta ctaccttcag aacggaagag atatgtacgt tgatcaggag cttgacatca
     6481 acagactttc tgattacgat gttgatcaca tcgttccaca gtctttcttg aaggatgatt
     6541 ctatcgataa caaggttctt acccgttctg ataagaacag aggaaagtct gataacgttc
     6601 catctgagga ggttgttaag aagatgaaga actactggag acagcttctt aacgctaagt
     6661 tgatcaccca gagaaagttc gataacctta ccaaggctga gagaggagga ctttctgagc
     6721 ttgataaggc tggattcatc aagagacagc ttgttgagac cagacagatc accaagcacg
     6781 ttgctcagat ccttgattct cgtatgaaca ccaagtacga tgagaacgat aagttgatca
     6841 gagaggttaa ggttatcacc ttgaagtcta agttggtttc tgatttcaga aaggatttcc
     6901 agttctacaa ggttagagag atcaacaact accaccacgc tcacgatgct taccttaacg
     6961 ctgttgttgg aaccgctctt atcaagaagt acccaaagtt ggagtctgag ttcgtttacg
     7021 gagattacaa ggtttacgat gttagaaaga tgatcgctaa gtctgagcag gagatcggaa
     7081 aggctaccgc taagtacttc ttctactcta acatcatgaa cttcttcaag accgagatca
     7141 cccttgctaa cggagagatc agaaagagac cacttatcga gaccaacgga gagaccggag
     7201 agatcgtttg ggataaggga agagatttcg ctaccgttag aaaggttctt tctatgccac
     7261 aggttaacat cgttaagaaa accgaggttc agaccggagg attctctaag gagtctatcc
     7321 ttccaaagag aaactctgat aagttgatcg ctagaaagaa ggattgggac ccaaagaagt
     7381 acggaggatt cgattctcca accgttgctt actctgttct tgttgttgct aaggttgaga
     7441 agggaaagtc taagaagttg aagtctgtta aggagcttct tggaatcacc atcatggagc
     7501 gttcttcttt cgagaagaac ccaatcgatt tccttgaggc taagggatac aaggaggtta
     7561 agaaggatct tatcatcaag ttgccaaagt actctctttt cgagcttgag aacggaagaa
     7621 agagaatgct tgcttctgct ggagagcttc agaagggaaa cgagcttgct cttccatcta
     7681 agtacgttaa cttcctttac cttgcttctc actacgagaa gttgaaggga tctccagagg
     7741 ataacgagca gaagcagctt ttcgttgagc agcacaagca ctaccttgat gagatcatcg
     7801 agcaaatctc tgagttctct aagagagtta tccttgctga tgctaacctt gataaggttc
     7861 tttctgctta caacaagcac agagataagc caatcagaga gcaggctgag aacatcatcc
     7921 accttttcac ccttaccaac cttggtgctc cagctgcttt caagtacttc gataccacca
     7981 tcgatagaaa aagatacacc tctaccaagg aggttcttga tgctaccctt atccaccagt
     8041 ctatcaccgg actttacgag accagaatcg atctttctca gcttggagga gataagagac
     8101 cagctgctac caagaaggct ggacaggcta agaagaagaa gtgagacgtc cgatcgttca
     8161 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     8221 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     8281 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     8341 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     8401 gatcgggaat tgatcccccc tcgacagctt ccggaaaggg cgaattcgca actttgtata
     8461 caaaagttgc cgagctcgcg gccgcatgtt gttgttacca gaaagtaaat aaatgttcaa
     8521 tctctgatgt tctcaagtaa gtgagtttta ttgggaataa tattaactta tgttcttctt
     8581 gcatttgatt tctttgccgc tctcttcttc tatcttaaat ctgtgtatac tatttcacta
     8641 ttgggctttt tattagtcta taatgggact caaaataagg ctttggccca catcaaaaag
     8701 ataagtcaca aatcaaaact aaattcagag tcttttctcc cacatcggtc actgtactca
     8761 ttttgtgttt gtttatatat tacacgaacc gatctttggt acgtgccaag aataagaaac
     8821 aaggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     8881 agtggcaccg agtcggtgct ttttttctag acccagcttt cttgtacaaa gttggcatta
     8941 ccatggcttc gttgaacaac ggaaactcga cttgccttcc gcacaataca tcatttcttc
     9001 ttagcttttt ttcttcttct tcgttcatac agtttttttt tgtttatcag cttacatttt
     9061 cttgaaccgt agctttcgtt ttcttctttt taactttcca ttcggagttt ttgtatcttg
     9121 tttcatagtt tgtcccagga ttagaatgat taggcatcga accttcaaga atttgattga
     9181 ataaaacatc ttcattctta agatatgaag ataatcttca aaaggcccct gggaatctga
     9241 aagaagagaa gcaggcccat ttatatggga aagaacaata gtatttctta tataggccca
     9301 tttaagttga aaacaatctt caaaagtccc acatcgctta gataagaaaa cgaagctgag
     9361 tttatataca gctagagtcg aagtagtgat tgttagctgg aatcatgttt acgttttaga
     9421 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     9481 gtcggtgctt tttttctaga cccagctttc ttgtacaaag ttggcattac tgcaggtcga
     9541 cgacccagct ttcttgtaca aagttggcat taccatggct tcgttgaaca acggaaactc
     9601 gacttgcctt ccgcacaata catcatttct tcttagcttt ttttcttctt cttcgttcat
     9661 acagtttttt tttgtttatc agcttacatt ttcttgaacc gtagctttcg ttttcttctt
     9721 tttaactttc cattcggagt ttttgtatct tgtttcatag tttgtcccag gattagaatg
     9781 attaggcatc gaaccttcaa gaatttgatt gaataaaaca tcttcattct taagatatga
     9841 agataatctt caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg
     9901 gaaagaacaa tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc
     9961 ccacatcgct tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg
    10021 attgttagct ggaatcatgt ttacgtttta gagctagaaa tagcaagtta aaataaggct
    10081 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttcta gacccagctt
    10141 tcttgtacaa agttggcatt actgcaggtc gacgacccag ctttcttgta caaagtggtt
    10201 cgataattct taattaacta gttctaatct agagtcctgc tttaatgaga tatgcgagac
    10261 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
    10321 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
    10381 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
    10441 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
    10501 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
    10561 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
    10621 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
    10681 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
    10741 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
    10801 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
    10861 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
    10921 cgacaacatg tcgaggctca gcaggacctg caggcatgca agctagtgaa gaatcgattt
    10981 gctacgtatt gcccctgcag gtccccagat tagccttttc aatttcagaa agaatgctaa
    11041 cccacagatg gttagagagg cttacgcagc aggtctcatc aagacgatct acccgagcaa
    11101 taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca aaagattcag
    11161 gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa gtactattcc
    11221 agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga ttggagtctc
    11281 taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa tagaggacct
    11341 aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac gactcaatga
    11401 caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact ccaaaaatat
    11461 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
    11521 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
    11581 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
    11641 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
    11701 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
    11761 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
    11821 atttcatttg gagagaacac gggggactct agaggatcca tgagcccaga acgacgcccg
    11881 gccgacatcc gccgtgccac cgaggcggac atgccggcgg tctgcaccat cgtcaaccac
    11941 tacatcgaga caagcacggt caacttccgt accgagccgc aggaaccgca ggagtggacg
    12001 gacgacctcg tccgtctgcg ggagcgctat ccctggctcg tcgccgaggt ggacggcgag
    12061 gtcgccggca tcgcctacgc gggcccctgg aaggcacgca acgcctacga ctggacggcc
    12121 gagtcgaccg tgtacgtctc cccccgccac cagcggacgg gactgggctc cacgctctac
    12181 acccacctgc tgaagtccct ggaggcacag ggcttcaaga gcgtggtcgc tgtcatcggg
    12241 ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg gatatgcccc ccgcggcatg
    12301 ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg tgggtttctg gcagctggac
    12361 ttcagcctgc cggtaccgcc ccgtccggtc ctgcccgtca ccgagatctg aacggagtgc
    12421 gcgtggcatc gcccgagttg gagctggtac gggaactcat cgaactcaac tggcataccc
    12481 gcaatggtga ggtggaaccg cggcggatcg cgtacgaccg tgcccaggag ggggtaccga
    12541 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
    12601 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
    12661 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
    12721 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
    12781 gcgcggtgtc atctatgtta ctagatcggg aattaattca ctggccgtcg ttttacaacg
    12841 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    12901 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    12961 cctgaatggc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    13021 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    13081 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
    13141 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    13201 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
    13261 cgatttcgga accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
    13321 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
    13381 ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
    13441 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
    13501 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
    13561 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
    13621 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
    13681 attgtaacga tgacagagcg ttgctgcctg tgatcaaata tcatctccct cgcagagatc
    13741 cgaattatca gccttcttat tcatttctcg cttaaccgtg acaggctgtc gatcttgaga
    13801 actatgccga cataatagga aatcgctgga taaagccgct gaggaagctg agtggcgcta
    13861 tttctttaga agtgaacgtt gacgatatca actcccctat ccattgctca ccgaatggta
    13921 caggtcgggg acccgaagtt ccgactgtcg gcctgatgca tccccggctg atcgacccca
    13981 gatctggggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc
    14041 cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga
    14101 acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc
    14161 agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc
    14221 cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg
    14281 acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt
    14341 ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag
    14401 atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg
    14461 cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg
    14521 aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa
    14581 cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg ttcgggctcc
    14641 acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag
    14701 accgacagcc caatgatctc gccgtcgatg taggcgccga atgccacggc atctcgcaac
    14761 cgttcagcga acgcctccat gggctttttc tcctcgtgct cgtaaacgga cccgaacatc
    14821 tctggagctt tcttcagggc cgacaatcgg atctcgcgga aatcctgcac gtcggccgct
    14881 ccaagccgtc gaatctgagc cttaatcaca attgtcaatt ttaatcctct gtttatcggc
    14941 agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag
    15001 tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg
    15061 accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca
    15121 ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca
    15181 cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct
    15241 cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact
    15301 tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga
    15361 tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca
    15421 gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta
    15481 ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt
    15541 cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt
    15601 actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt
    15661 aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg
    15721 ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc
    15781 gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct
    15841 gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct
    15901 cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact
    15961 tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg
    16021 cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct
    16081 ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg
    16141 cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct
    16201 tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg
    16261 caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct
    16321 tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc
    16381 gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg
    16441 cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat
    16501 cgttgcgcca catctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
    16561 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
    16621 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
    16681 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
    16741 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
    16801 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
    16861 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
    16921 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
    16981 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
    17041 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
    17101 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
    17161 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
    17221 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
    17281 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
    17341 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
    17401 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
    17461 cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgggtg atgctgccaa
    17521 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
    17581 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac
    17641 atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg
    17701 cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc
    17761 gggcaacagc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact
    17821 caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga
    17881 aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc tgttttacgc
    17941 gtatgacagt ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac
    18001 gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg
    18061 ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac
    18121 gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa
    18181 gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa
    18241 cataaaacac tatcaataag ttggagtcat tacccaatta tgatagaatt tacaagctat
    18301 aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct ttgcccattc
    18361 tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct tacatacggc
    18421 gatatcttct atataaaaga tatattatct tatcagtatt gtcaatatat tcaaggcaat
    18481 ctgcctcctc atcctcttca tcctcttcgt cttggtagct ttttaaatat ggcgcttcat
    18541 agagtaattc tgtaaaggtc caattctcgt tttcatacct cggtataatc ttacctatca
    18601 cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg agcacggcac ccgcgaccac
    18661 tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc
    18721 gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg
    18781 gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg
    18841 gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgctgc
    18901 catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc
    18961 cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc
    19021 acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag
    19081 gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt
    19141 ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct
    19201 gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc
    19261 aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa
    19321 atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag
    19381 cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg
    19441 cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgg
    19501 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
    19561 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcggtctt
    19621 g
//
