LOCUS       sequence-20315-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  TITLE     pBabe-IRES-EGFP plasmids
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe neo IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(53..627,628..630,631..639)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(220..237)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     447..466
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 tacgtaggat tcgccagcac agtggtcgac ggtaccgcgg gcccgggatc cgcccctctc
       61 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
      121 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
      181 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
      241 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
      301 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
      361 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
      421 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
      481 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tacacatgct
      541 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
      601 ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa gggcgaagag
      661 ctgttcaccg gggtggtgcc catcctggtc gagctggac
//
