LOCUS       sequence-203150-       13013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 13013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pLenti-Crispr-V2-HMID.v1
FEATURES             Location/Qualifiers
     source          1..13013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..465
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        467..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     620..640
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             683..863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    910..1035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1528..1761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1946..1990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2288..2405
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2456..2704
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2456..2476
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2627..2646
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2726..2801
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2863..3074
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3093..3102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3099..7202
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3099..7202
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7203..7250
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7251..7274
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7284..7340
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7341..7937
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7834..7854
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7953..8541
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8006..8026)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8424..8435)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8613..8846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8872..8889)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8878..9102
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9148..9576
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9235..9254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9445..9466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9585..9605)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9590..9919
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9770..9905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9832..9851
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9967..10014
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10033..10407
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10537..10658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10574..10593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10628..10647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10707..10723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10707..10723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10720..10742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10731..10747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10755..10761,10762..10779,10780..10785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10800..10821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10938..10955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11109..11697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11189..11208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11868..12659,12660..12728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12491..12510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12729..12833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12908..12927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
       61 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      661 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1381 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1441 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1501 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1561 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1621 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1681 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1741 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1801 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1861 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1921 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1981 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2041 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2101 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2161 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2221 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     2281 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2341 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2401 attttcgggt ttattacagg gacagcagag atccagtttg gttaattaag gtaccgaggg
     2461 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     2521 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     2581 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     2641 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
     2701 caccgggcac tgcggctgga ggtgggtttt agagctagaa atagcaagtt aaaataaggc
     2761 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgaa ttcgctagct
     2821 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     2881 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     2941 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3001 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3061 ccgccagaac acaggaccgg ttctagagcg ctgccaccat ggacaagaag tacagcatcg
     3121 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
     3181 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
     3241 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
     3301 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
     3361 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
     3421 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
     3481 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
     3541 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
     3601 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
     3661 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
     3721 ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc
     3781 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
     3841 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
     3901 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
     3961 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
     4021 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
     4081 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
     4141 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
     4201 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
     4261 aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
     4321 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
     4381 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
     4441 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
     4501 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
     4561 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
     4621 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
     4681 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
     4741 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
     4801 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
     4861 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
     4921 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
     4981 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
     5041 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
     5101 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
     5161 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
     5221 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
     5281 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
     5341 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
     5401 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
     5461 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
     5521 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
     5581 tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag agctttctga
     5641 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
     5701 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
     5761 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
     5821 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
     5881 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
     5941 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
     6001 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
     6061 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
     6121 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
     6181 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
     6241 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
     6301 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
     6361 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
     6421 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
     6481 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
     6541 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
     6601 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
     6661 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
     6721 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
     6781 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
     6841 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
     6901 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
     6961 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
     7021 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
     7081 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
     7141 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggcg
     7201 acaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa gattacaaag
     7261 acgatgacga taagggatcc ggcgcaacaa acttctctct gctgaaacaa gccggagatg
     7321 tcgaagagaa tcctggaccg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     7381 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     7441 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     7501 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7561 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7621 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7681 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggagtctcg cccgaccacc
     7741 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7801 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7861 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7921 gcaagcccgg tgcctgaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg
     7981 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     8041 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     8101 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8161 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8221 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8281 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8341 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8401 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8461 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8521 ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga
     8581 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     8641 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     8701 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8761 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8821 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     8881 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8941 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9001 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9061 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     9121 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     9181 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     9241 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9301 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9361 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9421 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9481 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9541 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9601 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9661 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9721 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9781 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9841 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     9901 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     9961 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
    10021 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
    10081 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
    10141 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
    10201 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
    10261 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
    10321 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
    10381 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
    10441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
    10501 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
    10561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
    10681 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    10741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    10801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    10861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    10921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    10981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    11041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    11101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    11161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    11221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    11281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    11341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    11401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    11461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    11521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    11581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    11641 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    11701 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    11761 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    11821 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    11881 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    11941 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    12001 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    12061 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    12121 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    12181 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    12241 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    12301 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    12361 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    12421 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    12481 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    12541 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    12601 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    12661 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    12721 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    12781 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    12841 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    12901 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    12961 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caa
//
