LOCUS       sequence-203157-       12448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for 3xFLAG-tagged NIPBL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12448)
  TITLE     Suske Lab plasmids
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pN3-Nipbl
FEATURES             Location/Qualifiers
     source          1..12448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             623..688
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    9236..9357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9273..9292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9327..9346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9364..9819)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9501..9522)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9713..9732
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9846..9950
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9952..10309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9952..10143
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9975..9995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10160..10295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10344..11138
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10344..11138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10398..10417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11008..11027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11326..11345)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11370..11417
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11746..12334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12235..12254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctccacca tggactacaa agaccatgac ggtgattata aagatcatga
      661 catcgactac aaggatgacg atgacaagaa gcttcgaatt ctgcagtcga cggtaccgcg
      721 ggccctgaat ggggatatgc cccatgtccc cataactacg cttgcgggga ttgctagtct
      781 tacggacctc ctgaaccagc tgcctctccc atctccttta cctgctacaa ctacaaagag
      841 cctcctcttt aattcacgaa tagcagaaga ggtgaactgc cttttggcct gtagggatga
      901 caatttggtt tcacagcttg tccatagtct caaccaggta tcaacagatc acatagaact
      961 gaaagataac cttggcagtg atgatcccga aggcgatata cccgtcttgt tgcaggccgt
     1021 cctggcaagg agtccgaacg ttttcaggga gaaaagcatg cagaacagat atgtacagag
     1081 tggaatgatg atgtctcagt ataaactttc tcagaattcc atgcacagta gtcctgcatc
     1141 ttccaattac caacaaacca ctatctcaca tagcccctcc agccggtttg tgccaccaca
     1201 gacaagctct gggaacagat ttatgccaca acaaaatagc ccagtgccta gtccatatgc
     1261 cccacagagt cctgcaggat acatgccata ttcccatcct tcaagttaca caacacatcc
     1321 gcagatgcag caagcatcgg tatcaagtcc tattgttgcc ggtggcttga gaaacatcca
     1381 tgataacaaa gtttctggtc cattatctgg caactcagct aaccatcatg ctgataaccc
     1441 tagacatggc tcaagtgacg actacctaca catggtgcac aggctaagta gtgatgatgg
     1501 cgattcttca acaatgagga atgctgcatc tttcccctta agatctcctc aaccagtgtg
     1561 ttctcctgct ggaagtgacg gaacacctaa aggctcaaga ccacctttaa ttctacagtc
     1621 tcagtcttta ccttgttcat cacctcgaga tgttcctcca gatatcttac tagattctcc
     1681 agaaagaaaa caaaaaaagc aaaagaaaat taaattaggc aaggatgaaa aagaccagaa
     1741 cgagaaagct gcaatgtatg atataattag ctctccaacc aaggactcca ctaaacttac
     1801 attaagactt tctcgtgtaa gatcttcaga tatggaccag caagacgata tgctatctgg
     1861 tatggaaaat agcaatgttt cagaaaatga tattcctttt aatgtgcagt atccaggaca
     1921 gacttcaaaa acacccatta ctccacagga cgtaaaccgc ccacttaatg ctgctcagtg
     1981 tttgtcgcag caagaacaaa cagcattcct tccagcaaat caagtgcctg ttttacaaca
     2041 gaacacttca gttgctacaa aacaaccaca gacttctgta gtacagaatc agcagcaggt
     2101 atcacaacag gggccgatat atgatgaagt cgaattggac gcgttggcag aaattgaacg
     2161 gatagagcga gaatcagcca ttgaaaggga gcgcttctca aaggaagttc aagataagga
     2221 taagcctttg aaaaaaagaa aacaagattc ttacccacag gaggctgggg gtgctacagg
     2281 aggtaataga ccagcttctc aggagacggg ttctacggga aatgggtcaa ggccagcatt
     2341 aatggttagc attgatcttc atcaggcagg aagagtggac tctcaggctt ctataactca
     2401 ggattcagac tccataaaaa agcctgaaga aaccaaacag tgtaatgatg cgcccatttc
     2461 tgttcttcag gaagatattg ttggaagtct taagtctata ccagaaaacc atcctgagac
     2521 tcctaaaaaa aagtctgatc ctgagctttc aaaaagcgaa atgaaacaaa atgaaagtag
     2581 gttgtcagaa tctaaaccaa atgaaaatca gctgggggag tcaaaatcaa atgaaagtaa
     2641 gttagaaact aaaactgaga ccccaacaga agaacttaag cagaacgaga acaaaacgac
     2701 agagtccaaa cagagcgaga gcgctgtagt tgagcctaaa cagaatgaaa atagaccgtg
     2761 tgacacaaaa ccaaatgaca acaaacagaa caacaccagg tcagaaaaca caaaagcaag
     2821 acctgaaacc ccaaagcaaa aagctgaaag ccggcctgaa accccaaagc aaaagagtga
     2881 aggccggcct gaaaccccaa aacagaaggg tgatggacga cctgagactc caaagcaaaa
     2941 gagtgaaggc cggcctgaaa ctccaaagca aaaaggtgaa ggccggcctg aaactccaaa
     3001 acataggcat gaaaatagga gggattctgg aaagccatca acagagaaga aacccgatgt
     3061 gtctaagcat aaacaggata tcaaatctga ctcacctcga ttaaaatcag aaagagctga
     3121 agccttaaag cagagacctg atgggcgatg ggagtcacta agacgtgacc atgatagtaa
     3181 acagaaatca gatgacaggg gtgagtcaga gcgacatcgg ggtgatcagt caagggttcg
     3241 aagaccagaa acattgagat cttctagtag aaatgaccat agcactaaat cagatggttc
     3301 aaaaactgaa aagctagaaa gaaaacacag gcatgaatca ggggactcac gggacagacc
     3361 ttctggggaa caaaaatcaa gaccagacag tcctcgtgta aaacaaggag atactaataa
     3421 atcaagacct ggttttaagt caccaaatag taaagatgac aaaaggacag agggtaacag
     3481 aagtaaagta gacagtaata aagcgcacac tgataataag gccgaatttc caagctattt
     3541 gttggggggg aggtctggtg cattgaaaaa tttcgtcatt ccaaaaatca agagggataa
     3601 agatggcaac ataactcagg aaactaagaa aatggacatg aaaggagagc agaaagacaa
     3661 agtagaaaaa atgggattag ttgaagatct aaacaaagga gctaagcctg tagttgttct
     3721 gcaaaaacta tcgctggatg atgtccagaa gctaattaaa gatagagagg agaaatcaag
     3781 aagttccctt aagtctatca agaataagcc atcaaagtca aataaaggta gtatagatca
     3841 atcagtgtta aaagaattgc cccctgagct cctggcagaa attgaatcta ccatgccact
     3901 ttgtgaacgt gtgaaaatga acaaacgcaa gcgtagcaca gttaatgaaa agccaaaata
     3961 tgctgaaatc agttcagatg aagataatga tagtgatgaa gcgtttgaat cctctaggaa
     4021 acggcataaa aaagatgacg ataaagcttg ggaatacgaa gagcgcgaca gaagaagctc
     4081 tggggatcat aggagaagtg gccactccca tgacggaagg aggagctcgg gtggtggtcg
     4141 ttaccgaaac cgaagtccgt ccgattctga catggaagat tactcccctc ctcccagcct
     4201 tagtgaggtt gctagaaaaa tgaagaaaaa agaaaaacaa aagaaaagga aagcttacga
     4261 gccaaagcta acgcccgaag aaatgatgga ttcttcaact tttaagagat ttacagcctc
     4321 aatagagaat attttggata atttagaaga tatggatttt actgcatttg gtgatgacga
     4381 tgagattcct caggaactgc ttttaggaaa acatcagctt aatgaacttg ggagtgaatc
     4441 tgctaaaatt aaagccatgg gtataatgga caagctttca actgacaaaa ctgtgaaagt
     4501 cctaaatata ttggagaaga atattcagga tggatcaaag ctttccactt tgttaaatca
     4561 taataatgat actgaagaag aagaaagatt gtggagagac ctaattatgg aaagagttac
     4621 aaagtcagca gatgcctgtc ttacaactat caacattatg acatctccta acatgccaaa
     4681 agctgtatac attgaagatg taattgaaag ggttatacag tacactaagt ttcatttaca
     4741 gaacacactc tatccccagt atgaccctgt ttacagagta gatccacatg gaggagggtt
     4801 attaagttca aaggccaaac gggctaaatg ttctactcat aagcagagag taattgtaat
     4861 gctttacaac aaagtttgtg acattgttag cagtttatca gaattgctag agatacagct
     4921 tcttacagac acaacaattc ttcaggtttc atcaatggga ataacaccct tttttgtgga
     4981 aaatgtcagt gaactacaat tgtgtgccat taagttagtc actgcagtat tttcaagata
     5041 tgaaaagcat aggcagttaa tattggaaga aatttttact tcacttgcaa gattaccaac
     5101 tagcaagaga agtttaagga atttcaggtt aaatagtagt gatgtggatg gagaacctat
     5161 gtatattcag atggttacag ctctagtttt acaacttatt cagtgcgtag ttcacttacc
     5221 atcatcggag aaggatccta attcagaaga agactcaaac aaaaaagttg atcaggatgt
     5281 tgtcattacg aactcttacg aaacagctat gagaacagcc caaaactttc tctccatctt
     5341 tcttaaaaaa tgtggtagta aacaaggaga agaagattac agaccgcttt ttgaaaactt
     5401 tgttcaagac cttctctcaa cagtcaataa gcccgagtgg ccggctgctg aactactcct
     5461 tagcttgtta gggaggctgt tggttcatca gttcagtaac aagtcaacag aaatggcttt
     5521 aagagtggca tctcttgatt accttgggac tgttgctgcg cgactgagaa aagatgctgt
     5581 caccagcaag atggatcaag ggtctataga acgaatttta aaacaggttt ctggaggaga
     5641 agatgagatc caacaattgc aaaaagcatt gcttgattat ctggatgaga acactgaaac
     5701 tgacccttcg ctagtgtttt ctcgtaaatt ctatatagcc cagtggttcc gtgacacaac
     5761 tctggaaaca gaaaaagcaa tgaaatcaca aaaagatgaa gaatcatctg acgcaacaca
     5821 tcatgcaaaa gaacttgaga caactgggca gatcatgcat cgcgctgaga accggaaaaa
     5881 gtttcttcga agcattatca aaaccacacc ttctcagttc agcacattaa agatgaactc
     5941 tgacactgtg gactatgatg atgcgtgctt gattgttcga tatttggcct ctatgaggcc
     6001 gtttgcccag agctttgata tttatttgac acagatacta cgagttcttg gtgaaaatgc
     6061 aattgccgtt cgaacaaaag ccatgaagtg tttgtctgag gttgttgctg tagaccccag
     6121 tattctagca aggcttgaca tgcagcgcgg agttcatggg cgactaatgg ataactccac
     6181 tagtgttcga gaggcagcag tggaattgct aggtcgcttt gtcctgtgtc gccctcagct
     6241 tgctgagcag tattatgaca tgctgattga aagaatattg gatactggga tcagtgtcag
     6301 gaaaagagta ataaagattt taagagacat ctgtattgaa caaccaacat ttccaaaaat
     6361 tacagaaatg tgtgtaaaaa tgattcgtag agtcaatgat gaagagggca ttaagaaatt
     6421 agtaaatgaa acattccaaa agctctggtt tactccaact ccacataatg acaaagaagc
     6481 aatgacaagg aagattttaa acatcactga tgtggttgca gcatgtagag atactggata
     6541 tgactggttt gagcaactcc ttcaaaattt gttgaagtct gaagaggatt cctcatataa
     6601 acctgtgaag aaagcttgta ctcaacttgt tgataaccta gttgaacata ttcttaaata
     6661 tgaggaatct ctagctgact cagacaataa aggtgtaaat tctggaagat tggtagcttg
     6721 tataaccact ttgttcttat tcagcaaaat aagaccccaa ctcatggtta aacatgccat
     6781 gactatgcag ccgtacctca ccactaagtg tagtacgcaa aatgatttca tggttatctg
     6841 caatgttgca aaaatccttg agttagttgt accactgatg gaacacccaa gtgaaacttt
     6901 ccttgccact attgaggaag atctgatgaa gctgatcatc aaatacggca tgactgtagt
     6961 acaacattgt gtgagctgtc ttggggctgt tgtaaataaa gtgacccaga attttaaatt
     7021 tgtgtgggcc tgtttcaata gatactatgg tgccatttca aaattaaaaa gtcagcacca
     7081 agaagaccca aataacactt cacttttaac aaataaacca gcgcttctta ggtccctctt
     7141 cactgttgga gcactttgcc gccattttga ttttgatctg gaagatttta aaggcaacag
     7201 taaggttaac ataaaggata aagtacttga actgttgatg tattttacaa aacactcaga
     7261 tgaagaagta caaacaaaag ccatcattgg tctaggattt gcctttatac agcatccgag
     7321 tctaatgttt gagcaagaag tgaagaatct gtataatagt attttatctg ataagaactc
     7381 ctcagtgaat ttaaaaatac aagtgttaaa aaacctccag acttacctac aagaagaaga
     7441 cacgcgaatg caacaagcag acagagactg gaagaaagtt gcaaaacagg aagacctgaa
     7501 ggaaatgggg gacgtctcct cggggatgag cagctccatc atgcagctgt acctcaagca
     7561 ggtgctggag gcgttctttc atacccagtc aagtgtgcgc cacttcgccc tgaatgtcat
     7621 tgctctgact ctgaaccaag gcctcatcca tccggttcag tgtgttccat acttaattgc
     7681 catgggaaca gatccagaac ctgctatgcg aaacaaggct gatcagcagc ttgtggagat
     7741 agacaaaaaa tatgctgggt tcatccatat gaaagccgtg gctggtatga agatgtctta
     7801 ccaggtccaa caggcgatca acacgtgcct aaaagaccct gtaaggggtt tccgacaaga
     7861 tgaatcctcc agtgctctgt gctcacatct atactccatg atccgcggga accgccaaca
     7921 cagacgcgcc tttcttattt ctttactcaa cctctttgat gacacagcaa aaacagaagt
     7981 gactatgctc ttgtacatag cagacaatct agcctgtttt ccataccaga ctcaggaaga
     8041 gccgctgttt ataatgcacc acatagacat tacgctctct gtttccggta gtaacctatt
     8101 gcagtcattc aaggagtcta tggtaaaaga caaaaggaaa gagaggaaaa cgtcccctgc
     8161 taaagagaac gagtccagtg agagtgaaga agaagtgtcc agacctcgga agtctcgaaa
     8221 gcgggtagat tcggagtcag actcagactc tgaagatgat ataaattcag tgatgaagtg
     8281 tttgccggaa aattcagccc ctttgattga attcgcaaat gtttcccagg gtattttatt
     8341 acttcttatg ttaaagcagc acctgaagaa tctctgtgga ttttctgata gtaaaattca
     8401 aaaatattct ccatctgaat ctgcaaaagt gtatgataaa gccattaatc ggaaaacagg
     8461 ggttcatttt catcccaaac aaacactgga cttcctgcga agtgacatgg ctaattccaa
     8521 actgacggag gatgtgaaga ggagcatcgt aaggcagtac ttagacttta aacttctcat
     8581 ggaacatcta gaccctgatg aagaagaaga ggaaggagag gtgtcagcta gcaccaatgc
     8641 tcggaacaaa gcaattacct cgctgcttgg aggaggcagc cctaaaaata acacagcagc
     8701 agacacagaa gatgaggaga gtgatgggga ggatagagga ggaggcactt cagggtcatt
     8761 gagaaggtca aaacgaaatt cagactctac ggagttggca gcacagatga atgaaagtgt
     8821 tgacgtcatg gatgtcatcg ctatttgctg tccaaagtac aaagaccgac cacagattgc
     8881 aagagtagta cagagaacca gcagtggcgt cagtgtccag tggatggcag gctcctacag
     8941 cggctcctgg acggaggcca agcgccgcga tggccgcaaa ctggtgcctt gggtagacac
     9001 tattaaagag tcagacatta tttacaaaaa aattgctcta acgagtgcta ataagctgac
     9061 taataaagtt gttcagactt tacgatccct gtatgccgcc aaggatggga cttccagcta
     9121 agtcgactag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     9181 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     9241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     9361 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     9421 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     9481 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     9541 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     9601 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     9661 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     9721 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     9781 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     9841 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9901 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     9961 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    10021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    10081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    10141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    10201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
    10321 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
    10381 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
    10441 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
    10501 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
    10561 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
    10621 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
    10681 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
    10741 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
    10801 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
    10861 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
    10921 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
    10981 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
    11041 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
    11101 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
    11161 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
    11221 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
    11281 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
    11341 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    11401 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
    11461 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
    11521 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
    11581 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    11641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    11701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11881 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    11941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12421 tgtggataac cgtattaccg ccatgcat
//
