LOCUS       sequence-203159-        6959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for full length murine Sp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6959)
  TITLE     Suske Lab plasmids
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pN3-Sp2FL
FEATURES             Location/Qualifiers
     source          1..6959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3747..3868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3784..3803)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3838..3857
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3875..4330)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4012..4033)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4224..4243
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4357..4461
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4463..4820
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4463..4654
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4486..4506)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4671..4806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4855..5649
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4855..5649
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4909..4928)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5519..5538
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5837..5856)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5881..5928
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6257..6845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6746..6765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacccacgc gtccgcccac
      661 gcgtccgccc acgcgtccgg gcggcggagg ccagggagga agatgtcgta atgagcgatc
      721 cacagatgag catggccgcc actgctgctg tcagtcccag tgactacctg cagcctgctg
      781 ctgctactac ccaggactcc cagccatctc ccttggccct gcttgctgcg acatgtagca
      841 aaattggccc cccagctgtt gaagctgcag tgacacctcc tgctcccccc cagcctaccc
      901 caaggaaact ggtccctatc aaacccgctc ctctccctct cagtccctgc aagaatagct
      961 ttagcatctt atcatctaaa ggaaatatac ttcagattca gggctcccag ctgagtacct
     1021 cctaccctgg agggcagttt gtgtttgcaa tccagaaccc caccctgatc aacaaaggga
     1081 gccgatcaaa tgcaagtatc cagtaccaag tccctcagat ccaagggaac agttcccaga
     1141 ccatccaggt gcagcccagt ctcaccaacc agatccaggt catcccaggc accaaccaag
     1201 ccatcaccac cccgtcaaca tctggtcata agcctgtccc catcaagcca gctcctgtcc
     1261 agaagtcaag tacgactacc acccctgtgc agagcggggc caacgtggtg aagctgacag
     1321 gcgggggtag caacatgacg ctcaccctgc cgctcaacaa cctggtgaac accagcgata
     1381 ttgggggccc tgctcagctc ctcactgaga gcccccccac cccactgtct aagactaaca
     1441 agaaagctag gaagaagagc ctccctgtgt cacagccatc cgtagctgtg gctgagcagg
     1501 tggagacggt gctgatagag accacggcag acaacatcat ccaggcagga aacaacctgc
     1561 tgatcgtcca gagccctggc gggggccagc cagctgtggt ccagcaagtc caggtggtgc
     1621 cacccaaggc tgagcagcag caggtggtcc agatccccca gcaggcactg cgggtggtgc
     1681 aagctgcctc tgccaccctc cccaccgtcc cccagaagcc ctctcagaac tttcagatcc
     1741 agacaactga accaacacca acccaggttt atatccgtac accttctggt gaggtacaga
     1801 cagtccttgt ccaggacagc cccccagcaa cagctgcaac cacatcaacc gtcacctgta
     1861 acagccctgc actccgagct ccccatctga gtggcaccag caaaaaacat tcagctgcca
     1921 ttctccgaaa agagcggccc ctgccaaaga ttgcacctgc tgggagcatc atcagcctga
     1981 acgccgcaca gctggcagca gctgctcagg ccatgcagac catcaacatc aatggtgtcc
     2041 aagtccaggg tgtgcctgtc accatcacca acactggcgg gcagcagcag ctgaccgtgc
     2101 agaacgtttc tggaaacaac ctgactatca gtgggctgag ccccacccag atccagctgc
     2161 agatggagca ggctctagcc ggagaggccc agcctgggga gaaacggcgg cgcatggcct
     2221 gcacgtgtcc caactgcaag gatggggaga agaggtctgg ggagcagggc aagaagaagc
     2281 acgtgtgcca catcccggac tgcgggaaga ctttccggaa gacatccttg ctccgggccc
     2341 acgtccgcct gcacactggc gagcggccct ttgtctgcaa ctggttcttc tgtggaaaga
     2401 gattcactcg gagcgatgaa ctccagcgac atgctcgaac ccacacaggg gacaagcgct
     2461 ttgagtgtgc ccaatgtcag aagcgcttca tgaggagtga ccacctcacc aagcattaca
     2521 agacccacct aggcacgaag ggcttgtaag gccaactgag gcaggaggcc ctgaagatgg
     2581 aacacctccc atctgattgg ccctgggtcc atgatggaca ggtgcccacg actgccctgg
     2641 gcagccctca ccccactcct gttctgtggc tgtgtccctg cagagaggga ctctgttcct
     2701 gtgttctcct ccttccaaag gctctgcccc tctcaccgtg agctcctggc ctgcccaggc
     2761 catggacact ggccatgccc aatgagacgt tctaacccag gacgcgtggg gacccttatt
     2821 tccaaaggaa aaacatacat ttcactccgt cgaggagcaa agtgagcccc catccccacc
     2881 ccatcccctt cctcaacact gccggagtcc cattgtgcca tgccccctcc cccaccgtgc
     2941 ctccttgtcc ccctcaatta agtcctcctg ggccgctagg gacacccagg ggccctgtac
     3001 ccagcctccc actgacaccc cccaccgggg aggtgggagg ctgcctccac tccactgccc
     3061 agccctgcca cggctctcct ccaaacattc caacttccta tgaaaggtca gacgccgagg
     3121 acctgccctc cctcttttct acggagaacg ttgccttata ctcacttcag atgatgacac
     3181 tgtggacgtg tgtgctttaa ccacatttta tttaattgct cccttcctct ctttgctaga
     3241 cgcctccccc ttgcttgcat tcagtttagg gttttggggg caatgctgag cataggaggg
     3301 tctttttttt tttgctattt tttttctcac tttaacaatt cttttctaaa tgacacattt
     3361 caactcaaat ctggactcac ctgagaacac cttccatcct gaccccgcca cgccctcatc
     3421 tccccctgcc caagctccac tgtgtacagt gtatattgta taatagacta ttgtgtctaa
     3481 gacatgtttt aaaaacatat tgcttgttat ttttgaggct tttaaattaa acagaaatcc
     3541 aactttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3601 aaaaaaaaaa aaaaaaaaaa aaaaagcggc cgcgactcta gatcataatc agccatacca
     3661 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     3721 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     3781 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3841 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
     3901 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3961 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     4021 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     4081 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4141 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4201 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4261 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4321 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4381 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4441 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     4501 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4561 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4621 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4681 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4741 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4801 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
     4861 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4921 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4981 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
     5041 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     5101 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     5161 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     5221 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     5281 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     5341 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
     5401 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     5461 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     5521 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     5581 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     5641 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     5701 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5761 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5821 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     5881 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     5941 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     6001 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     6061 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     6121 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6181 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6901 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
