LOCUS       sequence-20316-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  TITLE     pBabe-IRES-EGFP plasmids
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe bleo IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(63..637,638..640,641..649)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(230..247)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     457..476
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(695..716)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgtggtggt acgtaggaat tcgccagcac agtggtcgac ggtaccgcgg gcccgggatc
       61 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
      121 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
      181 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
      241 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
      301 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
      361 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
      421 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
      481 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
      541 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
      601 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaacca tggtgagcaa
      661 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      721 cggc
//
