LOCUS       sequence-203163-       13139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; 
            note plasmid contains 24xGCN4 repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13139)
  AUTHORS   Voigt F, Zhang H, Cui XA, Triebold D, Liu AX, Eglinger J, Lee ES, 
            Chao JA, Palazzo AF
  TITLE     Single-Molecule Quantification of Translation-Dependent Association 
            of mRNAs with the Endoplasmic Reticulum.
  JOURNAL   Cell Rep. 2017 Dec 26;21(13):3740-3753. doi: 
            10.1016/j.celrep.2017.12.008.
  PUBMED    29281824
REFERENCE   2  (bases 1 to 13139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.12"; journalName: "Cell Rep"; date: 
            "2017-12-26- 26"; volume: "21"; issue: "13"; pages: "3740-3753"
COMMENT     Sequence Label: pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24...
FEATURES             Location/Qualifiers
     source          1..13139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    73..91
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    109..127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    145..163
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    181..199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    217..235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     280..304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          381..513
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     496..515
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        558..576
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      580..589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             655..711
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             727..783
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             799..855
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             871..927
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             943..999
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1015..1071
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1087..1143
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1159..1215
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1231..1287
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1303..1359
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1375..1431
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1447..1503
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1519..1575
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1591..1647
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1663..1719
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1735..1791
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1807..1863
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1879..1935
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1951..2007
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2023..2079
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2095..2151
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2167..2223
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2311..2367
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2401..3330
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             3340..3363
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3697..3753
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5338..5403
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    7134..7268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7212..7231
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(7376..7409)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7462..7478)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7494..7516)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7927..8284
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             8349..8818
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9348..9364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9348..9364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9361..9383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9372..9388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9396..9402,9403..9420,9421..9426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9441..9462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9579..9596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9750..10338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9830..9849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10509..11300,11301..11369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11132..11151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11370..11474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11542..11560
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11598..11620)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11720..11739
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11933..11955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11947..11964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11948..11964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(13046..13093)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta
       61 tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg
      121 atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac
      181 tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta
      241 taagctttag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      301 gatcgcctgg agcaattcca caacactttt gtcttatacc aactttccgt accacttcct
      361 accctcgtaa aaccggtcag gtaagtatca aggttacaag acaggtttaa ggagaccaat
      421 agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc
      481 ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct
      541 cttaaggcta gagtacttaa tacgactcac tataggctag ccaccatggg cgccatcata
      601 cacgcggccg caatcatcgg tccgggtgga tctggaggtg gaggttctgg aggagaagaa
      661 cttttgagca agaattatca tcttgagaac gaagtggctc gtcttaagaa aggttctggc
      721 agtggagaag aactgctttc aaagaattac cacctggaaa atgaggtagc tagactgaaa
      781 aaggggagcg gaagtgggga ggagttgctg agcaaaaatt atcatttgga gaacgaagta
      841 gcacgactaa agaaagggtc cggatcgggt gaggagttac tctcgaaaaa ttatcatctc
      901 gaaaacgaag tggctcggct aaaaaagggc agtggttctg gagaagagct attatctaaa
      961 aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgaagag
     1021 ctactatcca agaattatca tcttgagaac gaggtagcgc gtttgaagaa gggttccggc
     1081 tcaggagagg aactgctctc gaagaactat catcttgaaa atgaggtcgc tcgattaaaa
     1141 aagggatcgg gcagtggtga ggaactactt tcaaagaatt accacctcga aaacgaagta
     1201 gctcgattaa agaaaggttc agggtcgggt gaagaattac tgagtaaaaa ttatcatctg
     1261 gaaaatgagg tagcgagact aaaaaagggg agtggttctg gcgaagagtt gctatcgaaa
     1321 aattatcatc ttgagaacga agttgctagg ctcaaaaagg gctcaggctc aggcgaggag
     1381 ttgctctcga aaaactacca cttggaaaat gaggtcgcga ggttgaaaaa ggggagcggg
     1441 tcgggcgagg agttattgag caaaaactat catttagaga acgaagtcgc gcgcttaaag
     1501 aaaggctcgg gctcgggcga agaactctta tcgaagaact accacctcga aaatgaggtc
     1561 gccaggttga aaaagggcag tggcagcggg gaggaactct tgagcaagaa ctaccacttg
     1621 gagaatgagg tcgcgagatt gaagaaaggg tcggggagcg gcgaggaatt gctcagcaag
     1681 aattatcatt tggagaacga agtcgccagg ctcaagaaag gctcggggtc gggggaggaa
     1741 ttgttgagta aaaactacca cttggaaaat gaagtcgcca ggctcaaaaa agggagtggg
     1801 agcggcgaag agttattgag caaaaattac cacttggaga acgaagtggc aaggctcaag
     1861 aaagggagcg gcagcgggga ggagctctta tcgaagaact accacttaga gaatgaagtc
     1921 gcccgcttga agaaaggctc ggggagcggg gaagagctct tgagcaagaa ctaccacttg
     1981 gaaaatgagg tggcgcgctt gaagaaaggg agcgggagcg gggaagagtt actatctaag
     2041 aattatcatc tcgagaacga ggtggctcga ctaaagaagg gctccggcag tggggaggaa
     2101 ctcctgtcga agaactatca tcttgaaaat gaggttgcaa gacttaaaaa ggggtccgga
     2161 tcaggtgagg aactactcag taagaattac cacctggaaa acgaagtagc tcgattaaag
     2221 aaaggttcag ggtcgggtga agaactgctc tcaaaagatt atcatttgga aaatgaggtt
     2281 gcacgtttaa aaaagggaag tggcagtggt gaggaacttc tgtcgaaaaa ttatcatctc
     2341 gagaatgaag tagcccgact taaaaagggt tctggctcgg gtcagcggcc gcaccggtca
     2401 gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc tcagtggtgg
     2461 gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga ttccgagaag
     2521 cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta cctgtggagg
     2581 cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct gatcggaatg
     2641 ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta caagtacctc
     2701 accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg ccacgactgg
     2761 ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa ggccatcgtc
     2821 catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga catcgaggag
     2881 gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa taacttcttc
     2941 gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga gttcgctgcc
     3001 tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc ctggcctcgc
     3061 gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg caactacaac
     3121 gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga ccctgggttc
     3181 ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt cgtgaaggtg
     3241 aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggtaagta catcaagagc
     3301 ttcgtggagc gcgtgctgaa gaacgagcag agcagcggtc tggaagttct gtttcagggc
     3361 ccgatggagg agccccctgt gcgagaagag gaagaggagg agggagagga ggacgaggag
     3421 agggacgagg ttgggcccga gggggcgctg ggcaagagcc ccttccagct gaccgccgag
     3481 gacgtgtatg acatctccta cctgttgggc cgcgagctta tggccctggg cagcgacccc
     3541 cgggtgacgc agctgcagtt caaagtcgtc cgcgtcctgg agatgctgga ggcgctggtg
     3601 aatgagggca gcctggcgct ggaggagctg aagatggaga gggaccacct caggaaggag
     3661 gtggaggggc tgcggagaca gtaaggggga agcggagcta ccaacttcag cctgctcaag
     3721 caggctggcg acgtggagga gaaccctgga cctatttgcg gccgctttac cccaagcacc
     3781 agaggatatg gcctcggtgc cagcgcatcc agcgacgtgc tttctctgtc aaacctgcaa
     3841 gccagcagag catatgggct cgctggctcc ttcaagaagt tcaggaaggg ggacgaatct
     3901 ctcgaaaggt ccagtggata tggccacgga cccgacgaag aacgcagcgg aagacccagc
     3961 gaggtcgacc atcccaccac accatatggg gtgggtggga ccacagtcat tacactggat
     4021 ctcatattca gcaatcagac cgacagtgca tatgggcacg tcggcaacga tcaactgctt
     4081 tacgacaaca ggcggcggtc acagtgcagc acagcatatg ggctggctgc acccgcccag
     4141 tggctggaca gagaagaggc ccccaaacac cacccagcca gaccatatgg ggtcggctgg
     4201 acatttccca aaccaaatct ccaccagaaa aaggtgggct cctggacagg atatggcctg
     4261 ccaggaagtt attccctgct cgtcgaatat ttctcaactc tgcaacggtg cagtccatat
     4321 ggggacgcac cattcaagaa attcaggaag ggggacgaga gtctcgaaga tgccaggaca
     4381 ccatatgggg tgcctggcag gagtggaagg ggctccgagg tcgacaacga ccagcttacg
     4441 cagagtggat atggccacct gcgaaagcat tttcccaaac ccaatgtcca caacaagaag
     4501 gtcacctcca cacgatatgg cgtgggaggt accgccagca gcgacgtgct cagtctgtca
     4561 aacgccatcc ctgccaccag atatggctgg ggcaggcgat caggccggcc cagcgaagtc
     4621 gacaacgacc aactgggcag caggtcatat gggaccgctg cctttgccaa gttccgcaag
     4681 ggggacgaga gtctcgagga cgacggcact ccatatgggg aggccgtctc tgcctcaagc
     4741 gatgttctct ctctttcaaa ccttcagtcc gacagaggat atggcctcgt cggaactggc
     4801 accacatcaa gggatacacc aggaccaaga aatcggacca cagcatatgg gctgggtccc
     4861 gctcacaacg accaccgcta tgtgtggaga aaaaaaagtc aggccagagc atatgggctc
     4921 ggcctgtcca ataccatccc taccgtcaag catggcgggg gctccatctg gaggagcaca
     4981 tatgggtgcc ctccaacccc ccctgggcgc aatctgtgct gtcgaatcgg cacagcctgc
     5041 agaccatatg gggtcgcagg cggccacaac gatcatcgat acgtgtggcg caagaaaggg
     5101 gcacgcacca gatatggctg ggcgtgcagt gccagcagcg acgtactcag cctgtccaac
     5161 ctcgaaacca gcaggtcata tgggaccgct ggttccgaag cctgcaaacc aaagaatacc
     5221 attccgacgg tcccctggac tgcatatggg cagccaggca agcatggggg agggtctatc
     5281 agatctggaa ggcccagcga gcccacagga tatggcctgg ggctcggctc cggatccgac
     5341 tacaaagacc acgacggcga ttacaaagat cacgatatcg attacaagga cgacgacgac
     5401 aagggcacac tgaacgtaat ccagacgcgt aagtcaggcc ggaaaattcc cgccaccgga
     5461 agttgagtag acggtgctgc ctgcgactca accccaggag gactgggtga acaaagctgc
     5521 gaagtgatcc atgtaagccc tcagaaccgt ctcggaaaga ggaccccaca tgttgtagct
     5581 tcaaggccca atgtcagacc acgccatggc gtgccactct gcggagagtg cagtctgcga
     5641 cagtgcccca ggaggactgg gtgaggatcc aacctacaaa cgggtggagg atcaccccac
     5701 ccgacacttc acaatcaagg ggtacaatac acaagggtgg aggaacaccc caccctccag
     5761 acacattaca cagaaatcca atcaaacaga agcaccatca gggcttctgc taccaaattt
     5821 atctcaaaaa actacaacaa ggaatcacca tcagggattc cctgtgcaat atacgtcaaa
     5881 cgagggccac gacgggagga cgatcacgcc tcccgaatat cggcatgtct ggctttcgaa
     5941 ttcagtgcgt ggagcatcag cccacgcagc caatcagagt cgaatacaag tcgactttcg
     6001 cgaagagcat cagccttcgc gccattctta cacaaaccac actctcccct acaggaacag
     6061 catcagcgtt cctgcccagt acccaactca agaaaattta tgtccccatg cagcatcagc
     6121 gcatgggccc caagaataca tccccaacaa aatcacatcc gagcaccaac agggctcgga
     6181 gtgttgtttc ttgtccaact ggacaaaccc tccatggacc atcaggccat ggactctcac
     6241 caacaagaca aaaactactc ttctcgaagc agcatcagcg cttcgaaaca ctcgagcata
     6301 cattgtgcct atttcttggg tggacgatca cgccacccat gctctcacga atttcaaaac
     6361 acggacaagg acgagcacca ccagggctcg tcgttccacg tccaatacga ttacttacct
     6421 ttcgggatca cgatcacgga tcccgcagct acatcacttc cactcaggac attcaagcat
     6481 gcacgatcac ggcatgctcc acaagtctca accacagaaa ctaccaaatg ggttcagcac
     6541 cagcgaaccc actcctacct caaacctctt cccacaaaac tggcaagcag gatcaccgct
     6601 tgcccattcc aacataccaa atcaaaaaca attactggta cagcatcagc gtaccagccc
     6661 acatctctca ctactatcaa aaaccaaacc gttcagcaac agcgaacggt acacacggaa
     6721 aaatcaactg gtttacaaat acgaaagacg atcacgcttt cgtccagcgc aaactattac
     6781 gaaaaacatc cgacgggaag agcaacagcc ttcccgcggc ggaaaacctc acaaaaacac
     6841 gacaaacgga tgcacgaaca cggcatccgc cgacaaccca caaacttaca accaggcaaa
     6901 cggtgcagga tcaccgcacc gtacatcaaa cacctcagat ctcatttcgc gcgggatcca
     6961 gaccacctcc cctgcgagct aagctggaca gccaatgacg ggtaagagag tgacattttt
     7021 cactaaccta agacaggagg gccgtcagag ctactgccta atccaaagac gggtaaaagt
     7081 gataaaaatg tatcactcca acctaagaca ggcgcagctt ccgagggatt tgagatccag
     7141 acatgataag atacattgat gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat
     7201 gccttatttg tgaaatttgt gatgctattg ccttatttgt aaccattata agctgcaata
     7261 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     7321 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctgaagt
     7381 tcctatacta tttgaagaat aggaacttcg gaataggaac ttcggatctc atatggccat
     7441 atgggggcgc ctagagaagg agtgagggct ggataaaggg aggatcgagg cggggtcgaa
     7501 cgaggaggtt caagggggag agacggggcg gatggaggaa gaggaggcgg aggcttaggg
     7561 tgtacaaagg gcttgaccca gggagggggg tcaaaagcca aggcttccca ggtcacgatg
     7621 taggggacct ggtctgggtg tccatgcggg ccaggtgaaa agaccttgat cttaacctgg
     7681 gtgatgaggt ctcggttaaa ggtgccgtct cgcggccatc cgacgttaaa ggttggccat
     7741 tctgcagagc agaaggtaac ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg
     7801 atccgctcga catctttcca gtgacctaag gtcaaactta agggagtggt aacaggctag
     7861 cccgggccca tattttcaga caaatacaga aacacagtca gacagagaca acacagaacg
     7921 atgctgcagc agacaagacg cgcggcgcgg cttcggtccc aaaccgaaag caaaaattca
     7981 gacggaggcg ggaactgttt taggttctcg tctcctacca gaaccacata tccctcctct
     8041 aaggggggtg caccaaagag tcctaaacga tcgggatttt aggactcagg tcgggccaca
     8101 aaaacggccc ccgaagtccc tgggacgtct cccagggttg cggccgggtg ttccgaactc
     8161 gtcagttcca ccacgggtcc gccagataca gagctagtta gctaactagt accgacgcag
     8221 gcgcataaaa tcagtcatag acactagaca atcggacaga cacagataag ttgctggcca
     8281 gcttacctcc cggtggtggg tcggtggtcc ctgggcaggg gtctcccgat cccggacgag
     8341 cccccaaatg aaagaccccc gctgacgggt agtcaatcac tcagaggaga ccctcccaag
     8401 gaacagcgag accacaagtc ggatgcaact gcaagagggt ttattggata cacgggtacc
     8461 cgggcgactc agtcaatcgg aggactggcg cgccgagtga ggggttgtgg gctcttttat
     8521 tgagctcggg gagcagaagc gcgcgaacag aagcgagaag cgaactgatt ggttagttca
     8581 aataaggcac agggtcattt caggtccttg gggcaccctg gaaacatctg atggttctct
     8641 agaaactgct gagggctgga ccgcatctgg ggaccatctg ttcttggccc tgagccgggg
     8701 caggaactgc ttaccacaga tatcctgttt ggcccatatt cagctgttcc atctgttctt
     8761 ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct
     8821 gttccatctg ttcctgacct tgatctgaac ttttctattc tcagttatgt atttttccat
     8881 gccttgcaaa atggcgttac ttaagctagc ttgccaaacc tacaggtggg gtctttcaca
     8941 tgtatatgtc aaaaataaaa atcaactaat tgactagtaa ttaatatgac tggcataatg
     9001 ggaaattgat cctgacagat gcaaactggc ttctcagcag cgcatttatg ttgtcaactg
     9061 aggaaggaaa cgttaatgac agaaactcta agtaatttcc acgtttatct atttttattt
     9121 atactagctt tggtaacagg aatattgcag cattcatgca cattgaaacc cttatgaaat
     9181 aaaaacatct gtgcatttaa aatggaatta acattttaaa tgttaaaaaa agctggctta
     9241 gcttcccccc gccccctagg gcatagaaca agtcaaatgc tttatatatt tgagtttggg
     9301 atgtattagg aaactcctaa gagcaaagct agcttggcgt aatcatggtc atagctgttt
     9361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     9421 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9661 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    10021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    10081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    10141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    10201 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    10261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    10321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    10381 aacgaaaact cacgttaagg gatttttgtc atgagattat caaaaaggat cttcacctag
    10441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10501 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10561 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10621 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10681 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10741 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10801 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10861 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10921 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10981 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    11041 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    11101 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    11161 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    11221 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    11281 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    11341 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    11401 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    11461 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    11521 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    11581 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11641 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11701 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    11761 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    11821 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    11881 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    11941 cgacgttgta aaacgacggc cagtgaatta attccaagct aagtgaaaga ctctgtctca
    12001 aaaaccaacc tgacacctga gccggagcgt tgcctctcac taccataaat atatatacac
    12061 aacacacaca cacacacaca cacacacaca cacacacaca cacacaatca tacacataaa
    12121 cacaatcaca cacacacaca tgcacaatca cacacacaca atcacacaca aacatacata
    12181 cacacaatca cacacaacca tacacacata tacacaatca cacacacaaa cacaatcaca
    12241 cacacacaca tcaatcaatc aatcaatcaa tataccaaaa atgatagctc atacctgtaa
    12301 tcttagatgt gggaaggcta aaacagcagg ctacccatga cttcaaggtc accctgggtt
    12361 acagagtagc taatagtcta gcttgaacta tagtgtagga tctttaaaaa aaaattaacc
    12421 aaccaaccaa caaacaaaca aacaaacaac aacaacaaca aatggaagac agaggacaaa
    12481 aaaaaataaa agaaaaagta gaaagccaat ggagatacta tcttaaaatt agaaatattt
    12541 atgacacatt ctgaatactc catgtttatt tcaaggaagc caatgtaact ttaagtacta
    12601 tggccattta atgtaaatac ttaagaaaaa aaaccaaatt aattttgata catgctgcat
    12661 gtgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctctc aaggaacagc
    12721 gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt acccgggcga
    12781 ctcagtcaat cggaggactg gcgccccgag tgaggggttg tgggctcgag ctcggggagc
    12841 agaagcgcgc gaacagaagt cgagctagct tgccaaacct acaggtgggg tctttcattc
    12901 cccccttttt ctggagacta aataaaatct tttattttat ctatggctcg tactctatag
    12961 gcttcagctg gtgatattgt tgagtcaaaa ctagagcctg gaccactgat atcctgtctt
    13021 taacaaattg gactaatccg gatctgaagt tcctatactt tctagagaat aggaacttcg
    13081 gaataggaac ttcgcggcca gatcttttgc ggccgctttg ggccggccga attctttac
//
