LOCUS       sequence-203193-        7523 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ 
            mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Koike K, Yoo SJ, Bleymehl K, Omura M, Zapiec B, Pyrski M, Blum T, 
            Khan M, Bai Z, Leinders-Zufall T, Mombaerts P, Zufall F
  TITLE     Danger perception and stress response through an olfactory sensor 
            for the bacterial metabolite hydrogen sulfide.
  JOURNAL   Neuron. 2021 Aug 4;109(15):2469-2484.e7. doi: 
            10.1016/j.neuron.2021.05.032. Epub 2021 Jun 28.
  PUBMED    34186026
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.05.032"; journalName: "Neuron"; date: 
            "2021-08-4- 4"; volume: "109"; issue: "15"; pages: "2469-2484.e7"
COMMENT     Sequence Label: Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV
FEATURES             Location/Qualifiers
     source          1..7523
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..435)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      complement(605..1193)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(685..704)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1364..2155,2156..2224))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2225..2329)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2355..2810)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2492..2513)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2704..2723
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2937..2959
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2951..2968
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2952..2968
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2978..2997
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2978..2996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3039..3055
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3039..3055
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
       61 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      121 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      181 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      241 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      301 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      361 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      421 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      481 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      541 tttctgggtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      601 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      661 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      721 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      781 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      841 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      901 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      961 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1021 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1081 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1141 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1501 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2341 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     2401 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2461 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2521 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2581 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2641 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2701 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2761 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     2821 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     2881 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     2941 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     3001 attggagctg tttaaacacc ggtggcggtg gcggccgctc tagaactagt ggatcccatg
     3061 cgagcagtgg gtatgagggt gacagaagct cctgtgagat agcttgtggg cagacagata
     3121 gctgggcctc accctgaaga cagttcagga ggtctagcat ggggtctgtg actctgaatt
     3181 gatggttagc tttaccacga ttctaataag ctgctgggtt caggagccat cataaagaca
     3241 gagcagtgga atccagaatt actaggggtg tgttagtctg tcacaagcat ttggttttgt
     3301 tagtctgcag aacctacttc acatagttgt tcttaggaat gcatcgaaca ggagtcagtc
     3361 ccgtgactcc agacgtttat atgacagcca ctatttgccc agggctcatt tggaaataaa
     3421 tgtgtagtta tgcacacaac tgtaatagtg catacagttc tacttatgtt tagtagctta
     3481 cagaaaaaga ccttctgtat gtagtgtgtg tcttagttac ttttctgtgg ttgtgattaa
     3541 acaccgggac ttacaaaaga agagtttctt ttggctaatg gctccatagt gatgagaatc
     3601 cggaatggca gagtagagac gggtggcact cagaatgcat atgcatgcca gctggagtag
     3661 gaagctgaag gcttaattgt aatcctcaaa cacaaagcag aggacctgaa ctaaaaatgg
     3721 tgtgagtctc taaactctca aagagcacat accagtcaca tactttctcc agcaagcaag
     3781 cccttgccta cccaaacagc accagcaact ggaggccatg tatccgaata catgagctga
     3841 ctggagacat gctcagtcaa accaccacag agagcatgag atgagcagaa caagaaagga
     3901 tctatgtagg cgtcttctca tagagcatca ggcactggtg gtaggcatgg gtgatgccgc
     3961 tacacacata gcacctggga agccttgtag ctgaggagac cctgctgtca ctggacagca
     4021 gaactgggca gagaatctgc tccgatcgct caagggcata taggttctgg aagttggagc
     4081 tgtgtttcct ccactacacg ggctcccttg cccatggaga agaaacagga gacagacctc
     4141 cagtgacacc ctctctccat atcctggaga tgccattgtg ccctttgtac cctcacttag
     4201 atttctcttt tctttgatgg aattgaaccc ttacctctct tccacagtca tcttcatttc
     4261 tgagacccat ggccccattt tttctggcca cactcggatt tggctagctt attgcctatc
     4321 cttcttccag agtcaggtaa tgtcacatag ctgaagggtg acccaggaaa attagataaa
     4381 ggtccaggga ttgagccaac aatatgcctc ttggctgcca aatattagaa tatgcaaagc
     4441 ttctgatagg gtcacactgg agtgcggcaa agtcttgcag atgaagaaga ctgaagagtt
     4501 tgtctgtgtc catgctttgt ccaaaccttc tggcccactc acagtgtggc agtgatggag
     4561 ccagatgtga tcttccctgt tttgttatct agggagtagg acagatgtag caatgcgtga
     4621 ggggaatagg atgattttat tctcaagtgt tagaatatct gcttgtaaac tcttatcaca
     4681 cctggtagac tcccagagcc ctgccctgat tagccaggaa tggttgagat ccaaccgcta
     4741 ttccccaaga cagtaacatt tctctagact ctgacctact ttcctagcaa aggccacagt
     4801 accatccccc atgccctctt aactggcagt ctagtgcaca cagtgatgtc tgcctagtgg
     4861 tatattgtct acagacactg ttttcattta tgtgtgtcca gtctgtttcc aagtgtgctg
     4921 cttttagaac tgggctgtgt ggtttgtcta accaaagagt atttctttgt taacatatac
     4981 ttcttactta tttttcataa cagaaagtac agataaacca aaggaagaaa atgaactccc
     5041 ctgtggttta tcacccagag agactaatgt tttaaaaact gagtgcacag ctttgtgtgc
     5101 acaaaggtat acatgatttc tcttacacaa acgggagtat cacacataca catgaatgtg
     5161 tgtttgtgtg catgtatttt tatactgctt tttactttcc attagtcttt gacataaatt
     5221 ttattttatt tttattttgt tttcacttgt atttttattt gtttttgtat tcatatacat
     5281 atctcatatg tatatgtgca taggtagttt gcttgcacat atgcgcatgc agcacatatg
     5341 tactgtgctt gtggaggcaa taatggaatg aaaaatcccc tgggactgga gttataggct
     5401 gctgtggact gccatttagg ttctgggaat taaatcctgc tcctctagaa aaatagccag
     5461 tgctcttaac tgctgagcca cctctccagt ccttgaaata tattctaaaa gagtaaggaa
     5521 attaaacaaa caaacaaaca aaaccagtgt ccttcacatg gtagcttagt tccctatcac
     5581 ttgtactgtt acatacccaa gctcacattt atatagtatt atatatcatt taaatggtat
     5641 caaattacag ttatcttata tttttgcata tatacagaat aacttcagtg accttgacat
     5701 tctgaaaatc ctaaggtttc ctctaaattc ttcaggaaca atgaagttca ttggatttag
     5761 gtttcatgtt tagattatcc cagctgtgtg ggggttagta attcatagac gataaacctg
     5821 ataatgaatc ccaagtgttt tcagtagaaa acaaacaaac aaaaacccca cactgcgttc
     5881 tgcaattctg cactgcgatg ctggcataaa agggaaccgg agctcttctg acacactctc
     5941 ccagcattca gtgtgcacgg cccatttctg agaagtttaa tcacttttga aattagagta
     6001 agtatcaggt ttccaagaaa cctagtagcg caataaaccc catagttaaa aatgaaagaa
     6061 ataatggtta ttaaaaataa taaaatcagc acaaaaacaa aaaggaaaag aaaatgcacc
     6121 aactctatta cttagatagg taacagttgc tggctgaaca tgattcagta tttgcgttca
     6181 tgtgtcctaa gtggtggcag agcggggaga ggccgtggct atcaggtgaa cctttagtgg
     6241 cacctgtact tagccactcc atttagacca cattccgtaa gaatgccata attgaaaaca
     6301 aaaatgtaca tgtgagagtt ctagggacct cgattccatg tcactttaga ccctccctaa
     6361 actctgcaca taactggtcc tctgtcgctg ccccgaggca ggttctgcca cccaggagat
     6421 cacctgcttt gctccatctt cacctcttct gtgtctgagg acacaagact ggagactggc
     6481 ccagaagccc cggatttctg agtcacctcc catattcatg aagtatttta tcaaataggg
     6541 cctttcacag tctgaagctg cctaacgctc ttcagctctt aaaacgaaac tgatcctgat
     6601 gcggatttgt ggagcagcct gaggtgtgaa cagcccccat cccacaccgt cgaaacctac
     6661 tgatgtgggg agagcagatt tgattgattg ctttctggga tgctagatgc actcaagcct
     6721 cagtccaaca cacatgggac caaatctaag atgctgcagg gtgggcaggc agcagagctg
     6781 gggacagcct ctgtcatccc gcctcctgta gccacagctc tctaatccca ctctctcctc
     6841 tcccaaacca catcccagag gcatcccagt cccagcctgc tctctggcct cccgcaggta
     6901 gacacattgg ctgttggagt ttttttctcc tcatgggacc ctgtgttaat agcttgatat
     6961 aaatcatcaa tccaattact agtttctccc attttacaga ttaggagcac aagactcagg
     7021 gagtaagtaa cttagcagtc aaggacaagc agtgccacat acccagtaca tggtaccaga
     7081 tctgaacctc atcccccatg gccttgctgt ttggggcaca gggacagttg tagaccagca
     7141 aggtcatgtg aatttgttca gcagtacagg cgtgtgacat ggtagctcag ctcagtagaa
     7201 caaatggtag agaagggtgg ggcttgtgga ggtacactta gctgtaaaga ctttattata
     7261 cagagcggtc agattgtgtt cctggggcct agctccagtg gcgtcccctt gcaggggagc
     7321 cagctggagt ccttagtcac tgacacttct gtcttctttt agcttctgat gaagtcacac
     7381 ttgctgggag tccagtggca gggcgagacg acacagatgc agtcaatagc cagtcatcac
     7441 cagatgagac caagacaagt gcctttgcca gtgaccctgt gttgtctgcc ttctgtgctg
     7501 tgttgtgagg cgcgccttaa tta
//
