LOCUS       sequence-203198-        9260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Flag-tagged full-length human POGZ protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Thanos Halazonetis
  TITLE     Expression plasmids for production of Rev7 and Champ1 proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDZ2FE_Pogz_1-1410
FEATURES             Location/Qualifiers
     source          1..9260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        472..675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     626..646
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     720..739
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        720..738
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             767..790
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             809..832
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5125..5142)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5131..5355
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5401..5829
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5488..5507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5698..5719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5838..5858)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5843..6172
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6023..6158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6220..6267
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6286..6660
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6790..6911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6827..6846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6881..6900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6960..6976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6960..6976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6973..6995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6984..7000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7008..7014,7015..7032,7033..7038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7053..7074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7191..7208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7362..7950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7442..7461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8121..8912,8913..8981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8744..8763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8982..9086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9161..9180)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
       61 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      121 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      181 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      361 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      421 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      481 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      541 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      601 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      661 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      721 aatacgactc actataggga gacccaagct ggctagcgcg acgatggact acaaggacga
      781 cgatgacaag ctcactggtt ctaatggtga ctacaaggat gacgatgaca agggtggtaa
      841 tggatccatg gcggacaccg acctgttcat ggaatgtgag gaggaggagt tggagccatg
      901 gcagaaaatc agtgatgtca ttgaggactc tgtagttgaa gattataatt cagtggataa
      961 aactaccaca gtttctgtga gccagcagcc agtctcggct ccagtgccca tcgctgccca
     1021 tgcttctgtt gctgggcacc tctctacatc caccaccgtt agtagcagcg gggcacagaa
     1081 cagcgacagt acaaagaaga ctcttgtcac actaattgcc aacaacaatg ctggcaatcc
     1141 tttggtccag caaggtggac agccactcat cctgacccag aatccagccc caggtctggg
     1201 cacaatggtt actcaaccag tattgaggcc tgttcaggtc atgcagaatg ccaatcatgt
     1261 gactagttcc cctgtggcct cacaaccaat atttatcact acgcagggat ttcctgtaag
     1321 gaatgtccgg cctgtacaaa atgcaatgaa tcaggttggg attgtgctga acgtacagca
     1381 aggccaaacg gttagaccaa ttacactagt tccagcccca ggtacccagt ttgttaagcc
     1441 gacagttgga gttccacaag tgttctccca gatgacccct gtgaggccag gctccacaat
     1501 gcctgtgagg cccaccacca acaccttcac caccgtcatc ccggccactc ttaccattcg
     1561 aagcaccgtc ccacagtccc agtcccagca gaccaagtcc actcccagca cttctaccac
     1621 tcccactgcc acacagccaa cctcactggg gcaactagct gttcagtctc caggccagtc
     1681 aaaccagacc acgaatccca agctagctcc ctccttcccc tctccacctg cagtgagcat
     1741 tgccagcttt gtcactgtga agcgacctgg tgttacaggc gaaaatagca atgaagtggc
     1801 caaattggtg aataccctta acaccatccc ttccctgggc cagagtcctg ggccagtggt
     1861 ggtgtccaac aacagctctg ctcatggctc tcaaagaacc agcggacctg agtcttcaat
     1921 gaaagtgacc tcttccatcc cagtatttga cctccaggat ggtggacgga aaatatgtcc
     1981 acgatgtaat gctcaatttc gtgttactga agctttgaga ggtcacatgt gttactgttg
     2041 cccagaaatg gttgaatacc agaagaaagg aaagtccctg gattcagaac ccagtgtccc
     2101 atcagcagca aagcccccat cccctgagaa aacagctcct gttgcttcca caccctcttc
     2161 tacacctatt cctgctctgt caccgcctac caaagtacca gaaccaaatg agaacgtggg
     2221 cgatgccgtc cagaccaaac tcattatgct tgtagatgac ttctactatg gacgggatgg
     2281 tggcaaagta gcccagctca caaatttccc taaggtcgcc acatctttcc gatgcccaca
     2341 ttgtaccaaa aggctaaaaa acaatattcg attcatgaac catatgaaac accacgtaga
     2401 actcgatcag cagaacggtg aggtagatgg tcatactatc tgccagcact gttaccgcca
     2461 gttttccact cccttccagc ttcagtgcca cttggaaaat gttcatagtc cctatgaatc
     2521 tactaccaag tgcaagatct gtgaatgggc gtttgaaagt gagccactat ttctccagca
     2581 tatgaaggat actcataagc ctggagagat gccttatgtt tgccaggtgt gtcaatatcg
     2641 ctcctcactc tactctgagg tagatgtcca ttttcggatg atccatgagg atacccggca
     2701 tctgctctgc ccttattgcc tgaaggtctt caaaaatggc aatgcattcc aacagcatta
     2761 catgaggcac cagaagagaa atgtttatca ctgcaacaaa tgccggctgc agtttctctt
     2821 tgccaaggac aaaattgaac acaagcttca acaccataaa accttccgta aacccaagca
     2881 gctggagggc ttgaaaccag gcaccaaggt gacaatccgg gcttcccgag ggcagccacg
     2941 aactgttcct gtatcctcta atgatacacc tcccagcgcc ttgcaggagg cagcaccgct
     3001 gacctcctca atggaccctc tgcctgtctt cctttatccc cctgtccagc gcagcatcca
     3061 gaagagagct gttaggaaaa tgagtgtcat gggccggcag acatgcctgg agtgcagctt
     3121 cgagatccca gacttcccta atcatttccc tacttacgta cactgctctc tgtgtcgcta
     3181 tagcacctgc tgttctcgag cttatgccaa ccacatgatc aacaatcatg ttccacggaa
     3241 gagccccaag tatttggctt tgtttaaaaa ttctgtgagt ggaatcaagc tggcctgcac
     3301 ttcatgtacc tttgttacct ctgtgggcga tgctatggcc aagcatttgg tattcaaccc
     3361 ctctcacaga tccagcagca tcctgccacg gggactcact tggatagctc actcaaggca
     3421 tggccagact cgtgaccgag tgcatgaccg gaacgtgaag aatatgtacc ctcctccttc
     3481 cttccccact aacaaagctg ccactgtgaa atctgcgggg gccaccccag ctgagcctga
     3541 agagctacta actcccttag ccccagcact cccatcacca gcctcaactg caaccccacc
     3601 accaaccccc actcacccgc aggctttagc ccttccaccg ctggctacag agggagccga
     3661 atgtctgaat gttgatgatc aggatgaagg gagcccagtc acccaagaac ctgagctagc
     3721 atcaggtggt ggtggtagtg gtggagttgg caaaaaggag cagctgtctg tgaagaagct
     3781 tcgagtagta ctgtttgctc tatgctgcaa tacagaacag gcagctgaac acttccgaaa
     3841 tccccagcga cgtattcgcc gttggcttcg acgtttccag gcctcccagg gggagaatct
     3901 agagggcaaa tatctgagct ttgaggcaga agagaaactg gctgagtggg tgctaaccca
     3961 gcgcgaacaa cagctacctg taaatgagga gaccttgttc cagaaggcca ccaaaatagg
     4021 acgttctttg gaaggggggt ttaagatctc ctatgagtgg gctgtgcgtt tcatgctgcg
     4081 gcaccacctg actccccatg cccggcgagc tgtggcccac accctaccta aggatgtagc
     4141 agagaatgca ggactcttca ttgattttgt acaacggcag attcacaacc aggacttacc
     4201 cttgtctatg attgtggcta ttgatgagat ctctttgttc ctggatacag aggtgctgag
     4261 cagtgatgat cgaaaggaga atgccctgca gacagtgggc acaggggaac cttggtgtga
     4321 tgtagtccta gccattctgg cagatggcac tgtccttccc accctggttt tctacagagg
     4381 gcagatggat cagcctgcta acatgccaga ctccatattg ctagaggcaa aggagagtgg
     4441 ctacagtgat gacgagatca tggagctgtg gtcaactcga gtgtggcaga agcacacagc
     4501 ttgccagcgt agcaaaggca tgcttgtgat ggactgtcat cgcactcact tgtcagaaga
     4561 ggtactggct atgcttagtg cctctagcac tttgcctgca gtggtcccag caggctgtag
     4621 ctccaaaatt cagccattag atgtatgcat caaaagaact gtcaagaact tcctgcataa
     4681 aaaatggaag gaacaggctc gggaaatggc agatactgca tgtgattctg atgtcctgct
     4741 tcagctggtg cttgtctggc tgggtgaagt gctaggtgtc attggggact gtccagagct
     4801 agttcagcgc tccttcctgg tggctagtgt tctgcctggc cccgatggca acattaactc
     4861 acctacaaga aatgctgaca tgcaggagga gctaattgcc tccctagagg agcaactgaa
     4921 gctgagtggg gaacattctg agtcttccac tccacgaccc agatcatctc ctgaagagac
     4981 aattgagcct gaaagtcttc accagctctt tgagggtgaa agtgagaccg agtctttcta
     5041 tggctttgaa gaagctgacc tagatctgat ggagatttga gcggccgctc gagtctagag
     5101 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5161 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5221 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5281 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5341 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5401 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5461 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5521 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5581 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5641 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5701 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5761 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5821 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5941 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6001 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6061 gccccatggc tgactaattt tttttattta tgcagaggcc gagaccgcct ctgcctctga
     6121 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6181 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     6241 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     6301 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     6361 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     6421 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     6481 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     6541 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     6601 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     6661 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     6721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     6781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     6961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9121 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9181 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9241 gcaaaattta agctacaaca
//
