LOCUS       sequence-203207-       12799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Gucy1b2-IRES-tauGFP mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: Gucy1b2-IRES-tauGFP-ACNF TV
FEATURES             Location/Qualifiers
     source          1..12799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4364..4375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6580..6613)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7288..7299
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7323..7343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8169..8234
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    8584..8632
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             9370..10173
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9433..9452)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10043..10062
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10180..10228
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(10255..10288)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(10335..11048,11049..11051,11052..11054))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(10338..11048,11049..11051,11052..11054))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10380..10401)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10729..10748
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     regulatory      11051..11060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    12236..12787
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     12626..12643
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gaaggttggg gatatttttc ttcctggttt tatgttttag tttgattttc agtttcagtg
       61 tgtgtttgcg tgcgcgcgct cgtgtgtgga cacagaggac ttttggaaac tggttccccc
      121 cttctactat gttggccctg gaaatcaaac tcaggttatc agacttgaca cccagggcct
      181 ttacctgctg acctaagtca ccaaccttca taataaccct aatagtaacc ctaataaaat
      241 aaacacataa catacaagga agtggatggc ttgaacagag gaaaaaaata attccaagtc
      301 ataatgtatt tttcctctca tagtgatccc agttccaggg atctattgac ccatcacagt
      361 atgtagtggc aaaagtttat tcatataaac tgcttgaagt tattatgcaa gactgcagtc
      421 tacagagttc attaatgagg agctaaatcc tgatgtgtca gaaaaatgtc ttcaagttgt
      481 ctaaattata tcatttgcta ccttaaattc agtggctaga accacagtgc ataattctct
      541 tctcgctctg taggctgatt aggcttagct gggagttccc aggtagaatt tatgtgtggt
      601 tataaccatg ttggctgggc tagagtcctc tggaggctct ccccctttgt ttctgagtat
      661 ttcatgttgg ccccactaca aaagtagcta gaaactggct aggggtctct caccatgagt
      721 tctctaacct tgtacttcta gatggttaga ctttgtgtgt tgggtgtgtg gaaggggatg
      781 ttacacatgt ggatgtgtgg tcgatgtaca tgtgtgtggg gccataggcc agaggagggt
      841 gttgggtgct ctaatgagtc attctccacc tggagctagg ctgggaggta aatcagaaga
      901 aggtggaagg aacacaaaaa aaccagctag aatgccacaa taatagccct aaggatacag
      961 tagtggcaca cacaccttgg ttgtaaccaa caactctcta agttggattt aagacccact
     1021 caacaggagg gaagccatgc ctggtagtgg taacctagct aactactcag tgctagcgaa
     1081 atcttggttc ttggaggaaa ctctacaacc actaatttac taaaccagca tgattcaaaa
     1141 caacaatcta tagacatatg tatacttaca ggtaagttag tgttgctata ctccttatca
     1201 gggacatttc tctttgcaac agatggagcc cactaaaaaa ccccataaat cagaattctg
     1261 gagccaaatc gcaaaggatg catctataaa acactcccca cctacagctc agggaacact
     1321 gcagaagctg gggtggaaag actgtaagag ccagaggaac agggagtttt ctgtgagact
     1381 gtgtttccta gtaacataag aagccacacc cataaagtct taccaacata cctgcccaaa
     1441 tgagagccca acaagttata gtatataggg gcatcatccc tgaggcctag taagacacta
     1501 agaatatttt aaaacaggtg atgctggcat cgtacaataa tgccttgaga tgggcacatt
     1561 cagtcgagat gggaaaggta cacgtgcagt gtccagcttc ccatcactaa tgacactgac
     1621 aatggtgagg ctctgaatgg cctccactgt ctctctactt agcacagagg aaaacacatg
     1681 aggacgggga gattatgact gcatactgtt tatttaccac ttgcaaatct gttttgttcc
     1741 taatgtgtcc agttttggta gatttataca atgtctctaa aacagggctt cacagtcttt
     1801 caaaattggc atttgtagct ttgcaaaggg agtaagagcc ctgcgtaaac ctgcttaatt
     1861 tgtttatcat atgctgagtt taaaaattac ttttgggata gagtgggggg tgaatatgac
     1921 caagacatat tttatacatg tatgaaattc tcaaaggata aatacaaata ttgtattttt
     1981 agaatcattt tttctgagag ttacagaatc ccctatattt ttctgttcta aactgccaca
     2041 gctgtgcata atctaaaacc acagctcagg gatctggggg agactccagg gtctgctgga
     2101 gtcaaagggt caaaaaacct ctaagtattt gctccacagc caacaggagc cctgactaga
     2161 cctggggaca ccgtagcctg tggtaaacta taaatgtatg ctggaacact taaagctggg
     2221 acttcatgtt tcagtctctt ttaatagctg gacgtctcca gtgaaaaatc acactgtaac
     2281 ttttctttag gtctctcctg ttctgttctt gacagcacat atctgactgg agctgctgtg
     2341 tcaggagtac agtctctgct tccaacacag agacggctct agccaatggc tgagagctaa
     2401 gatcagagtt ggggtagggt gggtctgaag gccaagtcct aggtctgtac ctcagtttcc
     2461 tgtctgcgca gtgtggctaa aaaccatacc tgtgtaatgc tcaaaggact tcagctgggt
     2521 accacaatgg tcctgctata tatgtgaacc caagttcctg ggtagatgct gatggcttag
     2581 ataggccact aagatctaag agagtgccat cattagaccc cactgaatgc agacatggaa
     2641 ctgctgtcat ttcctcctca cctcacatca agttgaaccc ctgaggggaa agaattcaga
     2701 atcctctgaa aattttcgga ctgcaccatg cagaaactca gagcagtttc tctttgggag
     2761 acagagtctc tcaagccacc tttaaagaat gattcagcca gttggttgac atatcattat
     2821 cacattttga agttaacaca gccccagagt ctatgaatgc tcaccccaca gttaattatt
     2881 cttgagtttt tctgtgctct caaaacatat tctacttatg taatgatccc tcgggggagc
     2941 accaggtgcc tttgtcccac agacagggcc agctgtgtaa aacctctgca tctgtcaggt
     3001 ttctctatgt ggcacataat tgcctgattt ccatctgaaa gtgcagcact gacattaaag
     3061 ccacatagaa cccaccattg ataacaatgg cttcccgaca cctagaagaa gcatgggggg
     3121 tgggggggca aataaacatg ctccccaaat gagcgcttgc cttcacaggg gctgggtctg
     3181 aatgtacagc tgcagatgaa gagaacaaac caagaggaat aaaggaaatg aaagctacag
     3241 attagcattc ggctgtctca gccagatggt cttctgggga gagcggtcct ccgcttgctt
     3301 tcttcagtct ccaggctagc acacagcacc cactctctgc aggggcagca gtgatctcac
     3361 ccctcagcca ggcaacccag tgaatcactc actctttctt tctcacacaa ctgtgagcca
     3421 atgaaaactt gaaagtgaaa gaaagactaa gaagggattg gggggtgggg gggcggggaa
     3481 gccaggcatc tgtgatgaat gaaaaagtct tagcctagga gagggctaag ataccaagac
     3541 tgagggaagt gtcgggaagc atcagctaag gaagaagaga gccactgaaa gagaaagctg
     3601 tagacacccc tcctcctcag cgcccagaag cattgcctcc agcatccagg attgccatgg
     3661 tattcaggac cttggggtcc tggcttttac aagcttgggc ttgaggtctg tgctcccaag
     3721 gtaaagaaag cagtggcctc ttctcaactt atttagaaac tgtcgcctcc ctccatggct
     3781 agacgccaat gagtctgcca gtcattggtc tgaggaccag ccatctgcca ccatctggat
     3841 agaccgtgcc tgggccaggt gccctgaaat ggaagaagct aggtggggca gaggcaaggg
     3901 agggggaggg gcaggagcaa ggcagggtta tggggtaaac tgagcctgca aagctgtagt
     3961 tgtagagggc agttccctgg aatgctctac tctgaaaagg gctctaccga cagtcaccac
     4021 caagccaagt gttcactgct catgagcaag cctcgtattt gagtggcagc ccaggtctgc
     4081 tgctggccat gagacagacc accagatggt gtgtgccagg gcatagatgt tctatgagct
     4141 aatcgatcca gtaggagtta atacatctaa cttgggtcca agggcctcaa ttcacagaag
     4201 attccagaac aagtgaaggt gcattttgag gttttagagg ccctgtgagc aagcacttta
     4261 gcagctcgcc aaacttccca agtgtggtgg tttggatgag aatggtcaac cttccccact
     4321 gtgatggact ctgagctctc tggaactgta agcacaaata aaaccttcct tctatgagtt
     4381 gcctcataga agtaacagaa gtagcataag taacatagca atagaaaagt aacaaacact
     4441 tccgaatacc aggagattgt ctgtagcagt gtttaataga ataaatgtca gctgcagggt
     4501 ggtacctatc acaggattgt ctttctttta cagctggaaa tgattattta aatcaaaata
     4561 aatgtggaac aaaaattgtc cttgtttttg gcctggacta tggtacctgt cgcacaattc
     4621 tgacagttct aaatataatg ttcaatgaag tagcatattg tactgtgacc ccagccaaac
     4681 aagcttgcaa caaaataatc caagatcccg ggctctggtg tttctccttc caaacttaac
     4741 tgaaaaggct tcccaccaaa tgaccaattt ggtcttggtc tcccaggtaa tgccacgtcc
     4801 tcagagtggt cgttctagct agggctttca ctcaagacat ggctagagag tttcaaagga
     4861 aagtagatgg agacagcaat gagctatgaa ttctgctgtc tttccttccc ctcacttaaa
     4921 cagggagatg ccacacttag gatcagtttt ttgtttgttt gtttgtttgt ttgttttgtt
     4981 ttgttttgtt tttcgagaca gggtttctct gtgtagccct ggctgtcctg gaactcactc
     5041 tgtagaccag gctggcctcg aactcagaaa tctgcctgcc tctgcctccc aagtgctagg
     5101 attaaaggca tgcgccacca cgcccggctt aggatcagtt tttaaatgcc ccaaaaggca
     5161 ggttatctga ttatccctaa cattgttgac ttgatggcac ttccaacata gagtggggat
     5221 tccaacgtga ctctgtatgt ctcctgggtc cactcctcca aactgccctc tctctctttc
     5281 tctctctctc cttctctctc cctctctctc cctctctctc tctctctctc tggtgtaacc
     5341 aagagtcagg ctgtttccca gcagtacgga ctcagactgt taactctgcc gtgctcccat
     5401 ttcctggtcc atcaagatga atctagatgg gctgtcaaac agggttatct ctagtaatta
     5461 gggtgcaagc aacatctgca tgcagggcca gtgcctggtg cgcagttgat gcatacatag
     5521 tgttaccaac cactgctacc atgagcactg ttgtcatctt aacttcatgg aattctcaca
     5581 aagcctgtta agttggactg ggggagagac ttagtgtggg taaaattctt gttgtgggta
     5641 tagggaccat gtgaaatcag gacgcctgta actccagttt gggagctgag acaggtggat
     5701 gctggaaggg gtacctgacc agtgtagcca aaccagcaaa ctccagattc agtgagagat
     5761 cttgcctcag aacatgaagt gggaagtgat agggggagac acacaaagtc aagacctgag
     5821 cacaactgca catgtccccc gccacgtcca ggacctgggt gttgggcagg acttggcctt
     5881 ggctctattc tttcccaaat ctgccctttg gctgttttca gtttgttttg ttttgtttgt
     5941 ttgtttgttt ttcttttcat tttacttttc agaaaaagat ctcctgggaa atgtggcttt
     6001 cgttctgttg ctttccagaa ttcccaggac tgggaaggga aaatggctac atttcacgct
     6061 gctggcccag tgccttccac agcacagctg tcataggggt accataaacc acagggtctg
     6121 ggtctctaac ccctccttct ctctcctccc actaccagtt aaacggcaca ctggagccca
     6181 aagtcagggt gtcctgtgaa agtggaccca gggcacctct ccagaggcaa gggttgggaa
     6241 gtggtggagc tgagagcaag caaacagaaa cagacccacc ggcaaagccg acatctagaa
     6301 caaacacagc ccaagtcagc tgaagattgg ccatgaacca tcactgcaaa gcattttcca
     6361 aacctccagg cttgtgtttc attcctaatt atttattctc aaaggggtcc tgaggaagat
     6421 atggaaagga ccacattttg aacttctgca tttttttaca agtcaatgtt ctttcctgcc
     6481 atgaatatag gaaatggatc ccagggcttc attttctccc ttgaggcgcg ccggtacctt
     6541 aattaagaat tcggatccga ttcgagctcg cgaaagctta taacttcgta tagcatacat
     6601 tatacgaagt tatggatctc catgggccag gcaaatatcc cttaccagcc tcacagagac
     6661 ctcccccacc ccccgcaacc ctagagttct tttactagtg agggacaagt ggacaatggt
     6721 gctgttgtgg gccccaccct gtgtcccctg tgcccacagt ggtcactctg cttggcaggc
     6781 aggtgttgca ggctggctgc tccaggccct ggcaggaggt actgaaggac ctggtaggct
     6841 cagatgccct ggatgccaag gcactgctgg agtacttcca accggtcagc cagtggctgg
     6901 aagagcagaa tcagcggaat ggcgaagtcc taggctggcc agagaatcag tggcgtccac
     6961 cgttacccga caactatcca gagggcattg gtaaagctct gagtgagggt ggactgggac
     7021 caagagaagt cctggcctct ggcctctggc ttctgggtca aagcctcagc atcctggtca
     7081 ctttgctgcc agctgagccc cagtgtcctt tgcttcagtg ccaagccacc cctgggctca
     7141 tcctcagggc cctaagcaga aatgggtatg tctttctctc agggtcctag agacagtgtg
     7201 cccaagcctg agggcccttg gggtcaggct ggctggcaca ttgctctatg aggtcacact
     7261 gcaggcttgg ctcttattgg ccggtgatgg gagcttcagg gctctgcttt cctgcggcca
     7321 tgcccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat
     7381 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
     7441 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
     7501 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
     7561 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
     7621 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt
     7681 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
     7741 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc
     7801 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta
     7861 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
     7921 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
     7981 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
     8041 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
     8101 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
     8161 gcgctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     8221 tttgtgtatt ttaggatgac tctggtcaga gatacctggc ctggtctgga cacagtgccc
     8281 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
     8341 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
     8401 caggggcaat ggtgcgcctg ctggaagatg gcgattagcc attaacgcgt aaatgattgc
     8461 tataattatt tgatatttat ggtgacatat gagaaaggat ttcaacatcg acggaaaata
     8521 tgtagtgctg tctgtaagca ctaatattca gtcgccagcc gtcattgtca ctgtaaagct
     8581 gagcggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcggtcgagc
     8641 tcgcgaaagc ttgggctgaa catcgagcgc cagggctccg taaagctact agagcacagg
     8701 cggtgcccca acgtcctggg gcctctccac taataacggc tacttccaat tgattggacg
     8761 cgccatcttg cctgccttat gcatattcag cggtgaactg aatattcatg aacgaggccc
     8821 gtcccgtccc tccctccttc cccccacccc cggaacccgc tccggaggac ccgaagggcc
     8881 ccgccttcat taccgatgcg taggacaaac cattttcccg atgtgtgtgg ggggatacta
     8941 atgagagact ttagctgaaa atgagcctga actccgaagc tgagtaaaaa tggcctaact
     9001 ttatcctccg ttctgtaagt cctcggtttg agtgcacggg aaacccgaaa ggaggacgac
     9061 aggaccagga acattctcct cctcctgtcg cgtcagaaag aacacccaac cagggagccg
     9121 gagccctagc gtcaacaact ccgccgcgcg cgctccgtgt aggccggtgc gggcggcccc
     9181 gtagcgcaag ggagggcggg aaaggaaggg gcgggacaca agggcgaatc tataaagggc
     9241 gtcactcagc cagttctctc ctcagaagcg ccgagagcgc gaccgggacg gttggagaag
     9301 aaggtggctc ccggaagggg gagagacaaa ctgccgtaac ctctgccgtt caggatccgt
     9361 gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct
     9421 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     9481 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     9541 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     9601 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     9661 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     9721 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     9781 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     9841 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9901 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     9961 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
    10021 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
    10081 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
    10141 gccttctatc gccttcttga cgagttcttc tgaggggatc ggcaataaaa agacagaata
    10201 aaacgcacgg gtgttgggtc gtttgttcgg atcgatccgt cgatcgacag atccataact
    10261 tcgtatagca tacattatac gaagttatga attcatcgat atctagatct cgaatctcta
    10321 gagtcgcggc cgctttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
    10381 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
    10441 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
    10501 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
    10561 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
    10621 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
    10681 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
    10741 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
    10801 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
    10861 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
    10921 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
    10981 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
    11041 cccttgctca ccatggtggc gaccggtgga tccccggaca cgatctccgc cccgtggtcg
    11101 gtcttggctt tggcgttctc gcggaaggtc agcttgtggg tttcgatctt tttattccct
    11161 ccgccaggga cgtgtgtgat gttgtccagg gacccaatct tcgactggac tctatccttg
    11221 aagtccagct tctcagattt cacttccact tggccacctc ctggcttatg atggatgttg
    11281 cctaatgagc cacacttgga ggtcaccttg ctcagatcca ctggtttgta gactatttgc
    11341 acactgccgc ctcctggcac gtgtttgata ttatcctttg agccacactt ggactggacg
    11401 ttgctaagat ccagcttctt attaattatc tgcaccttgc cacctcctgg ctggtgcttc
    11461 aggttttccg tggagccgat tttggacttg acgttcttca ggtctggcat gggcccggga
    11521 gcggcctgca ggcggctctt ggctgcagac ggcgacttgg ggggagtgcg gaccaccgcc
    11581 accttcttgg gctcccgggt gggcggggtc ggcagggagg gtgtgcggga gcggctgccc
    11641 ggagtgcctg gggagccggg gctgctgtag ccgctgcggt ccccggattt cccagattca
    11701 cctggcgagg tcttcggggt gggagtggtt tttgctggaa tccgggtggc gttggcctgg
    11761 cctttctggc ctggaggggc tgctccccgg ggtgtggcaa tcttcgttcc aggcttacca
    11821 tccgccccct tggttttttt gtcatcgggt ccagtcccat ctttgccttt actgaccatg
    11881 cgagcttggg tcacgtgtcc ggcagcttgg tcttccaggt tggacgtgtc gccaatgcct
    11941 gcttcttcag ctgcggttcc ttctgggatc tccgcggggg cctgagcggc cgcctgctca
    12001 ccgggggctc cctcatccac taagggtgct gtcgcatctt ccgccgtcgg agtgctctta
    12061 gcatcagagg tttcagagcc tggttcctca gatccatcat cggccggggt ctgcagggga
    12121 gactctttca ggccggggtc catgtcaccc tcctggtctt gcagggtgta gtccccctga
    12181 gcatgatctt ccatcacgtc gaactcctgg cggggctcag ccatggttgt ggccatatta
    12241 tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
    12301 taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca
    12361 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttagga gagccatttg
    12421 actctttcca caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt
    12481 gtatcttata cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc
    12541 tgcctgcaaa gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct
    12601 tccttcacga cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga
    12661 atgctcgtca agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat
    12721 atggtggaaa ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca
    12781 gtaacgttag ggggggggg
//
