LOCUS       sequence-203208-        5614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Gucy1b2-IRES-tauGFP mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: Gucy1b2-IRES-tauGFP-ACNF TV
FEATURES             Location/Qualifiers
     source          1..5614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     72..91
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(310..414)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(440..895)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(577..598)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     789..808
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1022..1044
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1036..1053
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1037..1053
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1063..1082
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1063..1081
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1124..1140
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1124..1140
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
       61 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      421 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
      481 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
      541 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
      601 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
      661 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
      721 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
      781 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
      841 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
      901 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
      961 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1021 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     1081 ggcgaattgg agctgtttaa acaccggtgg cggtggcggc cgctctagaa ctagtggatc
     1141 ccatgcgagc agtgggtatg agggtgacag aagctcctgt gagatagctt gtgggcagac
     1201 agatagctgg gcctcaccct gaagacagtt caggaggtct agcatggggt ctgtgactct
     1261 gaattgatgg ttagctttac cacgattcta ataagctgct gggttcagga gccatcataa
     1321 agacagagca gtggaatcca gaattactag gggtgtgtta gtctgtcaca agcatttggt
     1381 tttgttagtc tgcagaacct acttcacata gttgttctta ggaatgcatc gaacaggagt
     1441 cagtcccgtg actccagacg tttatatgac agccactatt tgcccagggc tcatttggaa
     1501 ataaatgtgt agttatgcac acaactgtaa tagtgcatac agttctactt atgtttagta
     1561 gcttacagaa aaagaccttc tgtatgtagt gtgtgtctta gttacttttc tgtggttgtg
     1621 attaaacacc gggacttaca aaagaagagt ttcttttggc taatggctcc atagtgatga
     1681 gaatccggaa tggcagagta gagacgggtg gcactcagaa tgcatatgca tgccagctgg
     1741 agtaggaagc tgaaggctta attgtaatcc tcaaacacaa agcagaggac ctgaactaaa
     1801 aatggtgtga gtctctaaac tctcaaagag cacataccag tcacatactt tctccagcaa
     1861 gcaagccctt gcctacccaa acagcaccag caactggagg ccatgtatcc gaatacatga
     1921 gctgactgga gacatgctca gtcaaaccac cacagagagc atgagatgag cagaacaaga
     1981 aaggatctat gtaggcgtct tctcatagag catcaggcac tggtggtagg catgggtgat
     2041 gccgctacac acatagcacc tgggaagcct tgtagctgag gagaccctgc tgtcactgga
     2101 cagcagaact gggcagagaa tctgctccga tcgctcaagg gcatataggt tctggaagtt
     2161 ggagctgtgt ttcctccact acacgggctc ccttgcccat ggagaagaaa caggagacag
     2221 acctccagtg acaccctctc tccatatcct ggagatgcca ttgtgccctt tgtaccctca
     2281 cttagatttc tcttttcttt gatggaattg aacccttacc tctcttccac agtcatcttc
     2341 atttctgaga cccatggccc cattttttct ggccacactc ggatttggct agcttattgc
     2401 ctatccttct tccagagtca ggtaatgtca catagctgaa gggtgaccca ggaaaattag
     2461 ataaaggtcc agggattgag ccaacaatat gcctcttggc tgccaaatat tagaatatgc
     2521 aaagcttctg atagggtcac actggagtgc ggcaaagtct tgcagatgaa gaagactgaa
     2581 gagtttgtct gtgtccatgc tttgtccaaa ccttctggcc cactcacagt gtggcagtga
     2641 tggagccaga tgtgatcttc cctgttttgt tatctaggga gtaggacaga tgtagcaatg
     2701 cgtgagggga ataggatgat tttattctca agtgttagaa tatctgcttg taaactctta
     2761 tcacacctgg tagactccca gagccctgcc ctgattagcc aggaatggtt gagatccaac
     2821 cgctattccc caagacagta acatttctct agactctgac ctactttcct agcaaaggcc
     2881 acagtaccat cccccatgcc ctcttaactg gcagtctagt gcacacagtg atgtctgcct
     2941 agtggtatat tgtctacaga cactgttttc atttatgtgt gtccagtctg tttccaagtg
     3001 tgctgctttt agaactgggc tgtgtggttt gtctaaccaa agagtatttc tttgttaaca
     3061 tatacttctt acttattttt cataacagaa agtacagata aaccaaagga agaaaatgaa
     3121 ctcccctgtg gtttatcacc cagagagact aatgttttaa aaactgagtg cacagctttg
     3181 tgtgcacaaa ggtatacatg atttctctta cacaaacggg agtatcacac atacacatga
     3241 atgtgtgttt gtgtgcatgt atttttatac tgctttttac tttccattag tctttgacat
     3301 aaattttatt ttatttttat tttgttttca cttgtatttt tatttgtttt tgtattcata
     3361 tacatatctc atatgtatat gtgcataggt agtttgcttg cacatatgcg catgcagcac
     3421 atatgtactg tgcttgtgga ggcaataatg gaatgaaaaa tcccctggga ctggagttat
     3481 aggctgctgt ggactgccat ttaggttctg ggaattaaat cctgctcctc tagaaaaata
     3541 gccagtgctc ttaactgctg agccacctct ccagtccttg aaatatattc taaaagagta
     3601 aggaaattaa acaaacaaac aaacaaaacc agtgtccttc acatggtagc ttagttccct
     3661 atcacttgta ctgttacata cccaagctca catttatata gtattatata tcatttaaat
     3721 ggtatcaaat tacagttatc ttatattttt gcatatatac agaataactt cagtgacctt
     3781 gacattctga aaatcctaag gtttcctcta aattcttcag gaacaatgaa gttcattgga
     3841 tttaggtttc atgtttagat tatcccagct gtgtgggggt tagtaattca tagacgataa
     3901 acctgataat gaatcccaag tgttttcagt agaaaacaaa caaacaaaaa ccccacactg
     3961 cgttctgcaa ttctgcactg cgatgctggc ataaaaggga accggagctc ttctgacaca
     4021 ctctcccagc attcagtgtg cacggcccat ttctgagaag tttaatcact tttgaaatta
     4081 gagtaagtat caggtttcca agaaacctag tagcgcaata aaccccatag ttaaaaatga
     4141 aagaaataat ggttattaaa aataataaaa tcagcacaaa aacaaaaagg aaaagaaaat
     4201 gcaccaactc tattacttag ataggtaaca gttgctggct gaacatgatt cagtatttgc
     4261 gttcatgtgt cctaagtggt ggcagagcgg ggagaggccg tggctatcag gtgaaccttt
     4321 agtggcacct gtacttagcc actccattta gaccacattc cgtaagaatg ccataattga
     4381 aaacaaaaat gtacatgtga gagttctagg gacctcgatt ccatgtcact ttagaccctc
     4441 cctaaactct gcacataact ggtcctctgt cgctgccccg aggcaggttc tgccacccag
     4501 gagatcacct gctttgctcc atcttcacct cttctgtgtc tgaggacaca agactggaga
     4561 ctggcccaga agccccggat ttctgagtca cctcccatat tcatgaagta ttttatcaaa
     4621 tagggccttt cacagtctga agctgcctaa cgctcttcag ctcttaaaac gaaactgatc
     4681 ctgatgcgga tttgtggagc agcctgaggt gtgaacagcc cccatcccac accgtcgaaa
     4741 cctactgatg tggggagagc agatttgatt gattgctttc tgggatgcta gatgcactca
     4801 agcctcagtc caacacacat gggaccaaat ctaagatgct gcagggtggg caggcagcag
     4861 agctggggac agcctctgtc atcccgcctc ctgtagccac agctctctaa tcccactctc
     4921 tcctctccca aaccacatcc cagaggcatc ccagtcccag cctgctctct ggcctcccgc
     4981 aggtagacac attggctgtt ggagtttttt tctcctcatg ggaccctgtg ttaatagctt
     5041 gatataaatc atcaatccaa ttactagttt ctcccatttt acagattagg agcacaagac
     5101 tcagggagta agtaacttag cagtcaagga caagcagtgc cacataccca gtacatggta
     5161 ccagatctga acctcatccc ccatggcctt gctgtttggg gcacagggac agttgtagac
     5221 cagcaaggtc atgtgaattt gttcagcagt acaggcgtgt gacatggtag ctcagctcag
     5281 tagaacaaat ggtagagaag ggtggggctt gtggaggtac acttagctgt aaagacttta
     5341 ttatacagag cggtcagatt gtgttcctgg ggcctagctc cagtggcgtc cccttgcagg
     5401 ggagccagct ggagtcctta gtcactgaca cttctgtctt cttttagctt ctgatgaagt
     5461 cacacttgct gggagtccag tggcagggcg agacgacaca gatgcagtca atagccagtc
     5521 atcaccagat gagaccaaga caagtgcctt tgccagtgac cctgtgttgt ctgccttctg
     5581 tgctgtgttg tgaggcgcgc cttaattaag aatt
//
