LOCUS       sequence-203210-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Knock out of EB1 in human cells by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   van Haren J, Charafeddine RA, Ettinger A, Wang H, Hahn KM, Wittmann 
            T
  TITLE     Local control of intracellular microtubule dynamics by EB1 
            photodissociation.
  JOURNAL   Nat Cell Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5.
  PUBMED    29379139
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2018 Jan 29. pii: 10.1038/s41556-017-0028-5. doi: 
            10.1038/s41556-017-0028-5."
COMMENT     Sequence Label: pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     105..124
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        204..279
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        376..661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tattaaatat tttgactgta cacaagatat tagtacaaaa tacgtgacgt agaaagtaat
       61 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      121 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      181 ccgaagatcg aacagttgtg ctcgttttag agctagaaat agcaagttaa aataaggcta
      241 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa
      301 atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat
      361 ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      421 cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg
      481 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      541 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat
      601 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      661 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
      721 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
      781 ggggcgcgcg ccaggcgggg cggggcgggg caaggggcgg ggcgggccga ggcgaagagg
      841 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggggg
//
