LOCUS       sequence-203216-       11832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Methanol induing RFP, estrogen inducing GFP, and IPTG inducing CFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Cao J, Perez-Pinera P, Lowenhaupt K, Wu MR, Purcell O, de la 
            Fuente-Nunez C, Lu TK
  TITLE     Versatile and on-demand biologics co-production in yeast.
  JOURNAL   Nat Commun. 2018 Jan 8;9(1):77. doi: 10.1038/s41467-017-02587-w.
  PUBMED    29311542
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-8"; volume: "9(1):77. doi"; pages: " 
            10.1038/s41467-017-02587-w"
COMMENT     Sequence Label: JC101
FEATURES             Location/Qualifiers
     source          1..11832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..435
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             459..479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1698..1847
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1854..1880
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      1922..2168
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             join(2734..2736,2737..2739,2740..3450)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2734..2736,2737..2739,2740..3450)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3475..3504
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3520..3537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3561..3807
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3822..4235
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        4242..4289
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4308..4682
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4748..4995
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4764..4782)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        5010..5948
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     terminator      6712..6958
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     terminator      8538..8784
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     protein_bind    9832..9848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    9857..9873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(9930..9932,9933..9935,9936..10649)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      10670..10916
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     rep_origin      complement(10983..11571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11063..11082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    11691..11740
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 tagctaaatt ggttccatca cctcgagtcc acacaccata gcttcaaaat gtttctactc
       61 cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc
      121 caagcacagc atactaaatt tcccctcttt cttcctctag ggtgtcgtta attacccgta
      181 ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc
      241 aataaaaatt tttatcacgt ttctttttct tgaaaatttt ttttttgatt tttttctctt
      301 tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt
      361 ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa agaaagcata
      421 gcaatctaat ctaaggacta gtaaaaatgc gcggagctcc taagaaaaag cgcaaagtcc
      481 ggccggcatc tagacccggg gagcgcccct tccagtgtcg catttgcatg cggaactttt
      541 cgcgccagga caggcttgac aggcataccc gtactcatac cggtgaaaaa ccgtttcagt
      601 gtcggatctg tatgcgaaat ttctcccaga aggagcactt ggcggggcat ctacgtacgc
      661 acaccggcga gaagccattc caatgccgaa tatgcatgcg caacttcagt cgccgcgaca
      721 acctgaaccg gcacctaaaa acccacctga ggaacatatg cggcggaggc acacctgcag
      781 ctgcgtcgac tctagaggat ccatctgctg gagacatgag agctgccaac ctttggccaa
      841 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
      901 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
      961 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     1021 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     1081 atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     1141 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     1201 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     1261 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     1321 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     1381 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     1441 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     1501 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     1561 tgcccctcta tgacctgctg ctggagatgc tggacgccca ccgcctacat gcgcccacta
     1621 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     1681 cttcatcgcc tagggccgac gcgctggacg atttcgatct cgacatgctg ggttctgatg
     1741 ccctcgatga ctttgacctg gatatgttgg gaagcgacgc attggatgac tttgatctgg
     1801 acatgctcgg ctccgatgct ctggacgatt tcgatctcga tatgttaatt aactacccgt
     1861 acgacgttcc ggactacgct tcttgaggta ccatcggtag accggtcttg ctagattcta
     1921 atcaagagga tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt
     1981 tttatttgta acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag
     2041 cttgctcctg atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa
     2101 atcattcgag tttgatgttt ttcttggcat ttcccactcc tcttcagagt acagaagatt
     2161 aagtgagagc tagcatatca tatagaagtc atcgcggcag atcaattcat caatcggagt
     2221 gaggatattg cgagtttacc accatcaatc ggagtgagga tctccaattg gtgacggtcc
     2281 agtcatcaat cggagtgagg attcagctgc ttctcgaggc cgcacatcaa tcggagtgag
     2341 gatgtacagg gtgggctgtt ccaccatcaa tcggagtgag gatttgcgtc aatggggcgg
     2401 agttcatcaa tcggagtgag gatatcgaag tcatcgagag cactcatcaa tcggagtgag
     2461 gatatactcc acccattgac gtcacatcaa tcggagtgag gattggaacc agaagggtct
     2521 cttcatcaat cggagtgagg ataccctatg ggcgcgccgt cgctggcaat aatagcgggc
     2581 ggacgcatgt catgagatta ttggaaacca ccagaatcga atataaaagg cgaacacctt
     2641 tcccaatttt ggtttcccct gacccaaaga ctttaaattt aatttatttg tccctatttc
     2701 aatcaattga acaactatca aaacacagaa ttcatggtga gcaagggcga ggagctgttc
     2761 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2821 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2881 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2941 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3001 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3061 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3121 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3181 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     3241 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3301 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagag cgccctgagc
     3361 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3421 atcactctcg gcatggacga gctgtacaag gccgcggcgg ccgccagctt tctagaacaa
     3481 aaactcatct cagaagagga tctgaatagc gccgtcgacc atcatcatca tcatcattga
     3541 ccggtcttgc tagattctaa tcaagaggat gtcagaatgc catttgcctg agagatgcag
     3601 gcttcatttt tgatactttt ttatttgtaa cctatatagt ataggatttt ttttgtcatt
     3661 ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc tcgcagctga tgaatatctt
     3721 gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt tcttggtatt tcccactcct
     3781 cttcagagta cagaagatta agtgagacct tcgtttgtgc ggatccccca cacaccatag
     3841 cttcaaaatg tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc
     3901 cgtaccactt caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg
     3961 gtgtcgttaa ttacccgtac taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt
     4021 tttcttcgtc gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt
     4081 ttttttgatt tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtct
     4141 tcaatttctc aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg
     4201 ctcattagaa agaaagcata gcaatctaat ctaagggcgg tgttgacaat taatcatcgg
     4261 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     4321 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     4381 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     4441 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     4501 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     4561 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     4621 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     4681 gacacgtccg acgcggcccg acgggtccga ggcctcggag atccgtcccc cttttccttt
     4741 gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct
     4801 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata
     4861 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga
     4921 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg
     4981 aaggctttaa tttgcaagct ggagaccaag atctaacatc caaagacgaa aggttgaatg
     5041 aaaccttttt gccatccgac atccacaggt ccattctcac acataagtgc caaacgcaac
     5101 aggaggggat acactagcag cagaccgttg caaacgcagg acctccactc ctcttctcct
     5161 caacacccac ttttgccatc gaaaaaccag cccagttatt gggcttgatt ggagctcgct
     5221 cattccaatt ccttctatta ggctactaac accatgactt tattagcctg tctatcctgg
     5281 cccccctggc gaggttcatg tttgtttatt tccgaatgca acaagctccg cattacaccc
     5341 gaacatcact ccagatgagg gctttctgag tgtggggtca aatagtttca tgttccccaa
     5401 atggcccaaa actgacagtt taaacgctgt cttggaacct aatatgacaa aagcgtgatc
     5461 tcatccaaga tgaactaagt ttggttcgtt gaaatgctaa cggccagttg gtcaaaaaga
     5521 aacttccaaa agtcggcata ccgtttgtct tgtttggtat tgattgacga atgctcaaaa
     5581 ataatctcat taatgcttag cgcagtctct ctatcgcttc tgaaccccgg tgcacctgtg
     5641 ccgaaacgca aatggggaaa cacccgcttt ttggatgatt atgcattgtc tccacattgt
     5701 atgcttccaa gattctggtg ggaatactgc tgatagccta acgttcatga tcaaaattta
     5761 actgttctaa cccctacttg acagcaatat ataaacagaa ggaagctgcc ctgtcttaaa
     5821 cctttttttt tatcatcatt attagcttac tttcataatt gcgactggtt ccaattgaca
     5881 agcttttgat tttaacgact tttaacgaca acttgagaag atcaaaaaac aactaattat
     5941 tcgaaacgaa ttcatggcaa cttccggtat ggtctcaaag ggagaggaaa ataatatggc
     6001 tattattaag gagtttatgc gttttaaggt acatatggaa ggttctgtca acggtcacga
     6061 attcgaaatt gaaggtgagg gggaggggag accatacgag ggaactcaga ctgctaagtt
     6121 aaaggtcact aaaggtggtc ctttaccttt cgcctgggat atcctgtctc cacagtttat
     6181 gtacggttca aaggcttatg tgaaacatcc tgccgatatc ccagattatc ttaaactttc
     6241 tttccctgag ggttttaagt gggagagggt aatgaacttt gaagacggtg gtgtggtcac
     6301 tgttactcag gactcaagtc tgcaggacgg tgagttcatc tacaaggtga agctgagagg
     6361 taccaatttt ccatcagatg gtcccgtgat gcaaaaaaag acaatgggtt gggaagcttc
     6421 tagtgaacgt atgtatcccg aagatggagc tttgaaaggt gaaattaagc aaagactaaa
     6481 acttaaggat ggtggacatt acgatgctga agttaagacg acctacaagg ccaaaaagcc
     6541 agtccagttg cctggagcat acaatgttaa catcaaattg gatataactt cccataatga
     6601 agactatacc atcgtcgagc aatacgaacg agccgaaggg agacacagta ctggtggtat
     6661 ggatgaactt tataaaggat ccggaaccgc accggtcttg ctagattcta atcaagagga
     6721 tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta
     6781 acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg
     6841 atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag
     6901 tttgatgttt ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagaat
     6961 aactgtcgcc tcttttatcc tcttcagagt acagaagatt aagtgagaat aactgtcgcc
     7021 tcttttatct gccgcactgc atgaggtgtc cccttagtgg gaaagagtac tgagccaacc
     7081 ctggaggaca gcaagggaaa aatacctaca acttgcttca taatggtcgt aaaaacaatc
     7141 cttgtcggat ataagtgttg tagactgtcc cttatcctct gcgatgttct tcctctcaaa
     7201 gtttgcgatt tctctctatc agaattgcca tcaagagact caggactaat ttcgcagtcc
     7261 cacacgcact cgtacatgat tggctgaaat ttccctaaag aatttctttt tcacgaaaat
     7321 ttttttttac acaagatttt cagcagatat aaaatggaga gcaggacctc cgctgtgact
     7381 cttctttttt ttcttttatt ctcactacat acattttagt tattcgccaa cgatgctcga
     7441 gaaaccagta acattgtatg atgtcgcaga gtatgccggt gtctcttatc agactgtttc
     7501 cagagtggtg aaccaggcca gccatgtttc tgccaaaacc agggaaaaag tggaagcagc
     7561 catggcagag ctgaattaca ttcccaacag agtggcacaa caactggcag gcaaacagag
     7621 cttgctgatt ggagttgcca cctccagtct ggccctgcat gcaccatctc aaattgtggc
     7681 agccattaaa tctagagctg atcaactggg agcctctgtg gtggtgtcaa tggtagaaag
     7741 aagtggagtt gaagcctgta aagctgctgt gcacaatctt ctggcacaaa gagtcagtgg
     7801 gctgatcatt aactatccac tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     7861 taatgttcca gcactctttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     7921 ctcccatgaa gatggtacaa gactgggtgt ggagcatctg gttgcattgg gacaccagca
     7981 aattgcactg cttgcgggcc cactcagttc tgtctcagca aggctgagac tggctggctg
     8041 gcataaatat ctcactagga atcaaattca gccaatagct gaaagagaag gggactggag
     8101 tgccatgtct gggtttcaac aaaccatgca aatgctgaat gagggcattg ttcccactgc
     8161 aatgctggtt gccaatgatc agatggcact gggtgcaatg agagccatta ctgagtctgg
     8221 gctgagagtt ggtgcagata tctcggtagt gggatacgac gataccgaag acagctcatg
     8281 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     8341 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgccagt
     8401 ctcactggtg aagagaaaaa ccaccctggc acccaataca caaactgcct ctccccgggc
     8461 attggctgat tcactcatgc agctagcaag acaggtttcc agactggaaa gtgggcagtg
     8521 atcttgctag attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct
     8581 tcatttttga tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg
     8641 tttcttctcg tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg
     8701 gtaggggttt gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt
     8761 cagagtacag aagattaagt gagatcaatt cttgatttag tatacacata accaaatttg
     8821 gatcaagttt gaagtaaaac tttaacttca gctccttaca tttgcactaa gatctctgct
     8881 actctggtcc caagtgaacc accttttgga ccctattgac cggaccttaa cttgccaaac
     8941 ctaaacgctt aatgcctcag acgttttaat gcctctcaac acctccaagg ttgctttctt
     9001 gagcatgcct actagggact ttaacgaact gtggggttgc agacagtttc aggcgtgtcc
     9061 cgaccaatat ggcctactag actctctgaa aaatcacagt tttccagtag ttccgatcaa
     9121 attaccatcg aaatggtccc ataaacggac atttgacatc cgttcctgaa ttatagtctt
     9181 ccaccgtgga tcatggtgtt cctttttttc ccaaagaata tcagcatccc ttaactacgt
     9241 taggtcagtg atgacaatgg accaaattgt tgcaaggttt ttctttttct ttcatcggca
     9301 catttcagcc tcacatgcga ctattatcga tcaatgaaat ccatcaagat tgaaatctta
     9361 aaattgcccc tttcacttga caggatcctt ttttgtagaa atgtcttggt gtcctcgtcc
     9421 aatcaggtag ccatctctga aatatctggc tccgttgcaa ctccgaacga cctgctggca
     9481 acgtaaaatt ctccggggta aaactttaat gtggagtaat ggaaccagaa acgtctcttc
     9541 ccttctctct ccttccaccg cccgttaccg tccctaggaa attttactct gctggagagc
     9601 ttcttctacg gcccccttgc agcaatgctc ttcccagcat tacgttgcgg gtaaaacgga
     9661 ggtcgtgtac ccgacctagc agcccaggga tggaaaagtc ccggccgtcg ctggcaataa
     9721 tagcgggcgg acgcatgtca tgagattatt ggaaaccacc agaatcgaat ataaaaggcg
     9781 aacacctttc ccaattttgg tttctcctga cccaaagact ttaaattgga attgtgagcg
     9841 gataacaatt tcggaattgt gagcggataa caatttaatt tatttgtccc tatttcaatc
     9901 aattgaacaa ctatcaaaac acagaattca tggtgagcaa gggcgaggag ctgttcaccg
     9961 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
    10021 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
    10081 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
    10141 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
    10201 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
    10261 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
    10321 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg
    10381 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
    10441 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
    10501 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
    10561 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
    10621 ctctcggcat ggacgagctg tacaagtaac cggtcttgct agattctaat caagaggatg
    10681 tcagaatgcc atttgcctga gagatgcagg cttcattttt gatacttttt tatttgtaac
    10741 ctatatagta taggattttt tttgtcattt tgtttcttct cgtacgagct tgctcctgat
    10801 cagtctatct cgcagctgat gaatatcttg tggtaggggt ttgggaaaat cattcgagtt
    10861 tgatgttttt cttggtattt cccactcctc ttcagagtac agaagattaa gtgagacctt
    10921 cacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10981 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11041 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11101 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11161 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11221 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11281 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11341 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11401 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    11461 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11521 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11581 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11641 gtcatgagat cagatctgag ctcgtttggc cgtggccgtg ctcgtcctcg tcggccggct
    11701 tgtcgacgac ggcggtctcc gtcgtcagga tcatccgggc cacaagcttg ctgacagaag
    11761 cctcaagaaa aaaaaaattc ttcttcgact atgctggagg cagagatgat cgagccggta
    11821 gttaactata ta
//
