LOCUS       sequence-203223-       11965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Methanol inducing hGH, estrogen inducing G-CSF, and IPTG inducing 
            Interferon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11965)
  AUTHORS   Cao J, Perez-Pinera P, Lowenhaupt K, Wu MR, Purcell O, de la 
            Fuente-Nunez C, Lu TK
  TITLE     Versatile and on-demand biologics co-production in yeast.
  JOURNAL   Nat Commun. 2018 Jan 8;9(1):77. doi: 10.1038/s41467-017-02587-w.
  PUBMED    29311542
REFERENCE   2  (bases 1 to 11965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-8"; volume: "9(1):77. doi"; pages: " 
            10.1038/s41467-017-02587-w"
COMMENT     Sequence Label: JC031
FEATURES             Location/Qualifiers
     source          1..11965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        65..471
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             495..515
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1734..1883
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1890..1916
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      1958..2204
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             join(2765..2821,2822..3019)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     CDS             3566..3595
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3611..3628
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3652..3898
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3913..4326
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        4333..4380
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4399..4773
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      4839..5086
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4855..4873)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        5101..6039
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             join(6040..6096,6097..6294)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     terminator      6892..7138
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     terminator      8668..8914
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     protein_bind    9962..9978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    9987..10003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             join(10066..10122,10123..10320)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR"
     terminator      10839..11085
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     rep_origin      complement(11152..11740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11232..11251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    11860..11909
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 gaggcagaga tgatcgagcc ggtagttaac tatatatagc taaattggtt ccatcacctc
       61 gagtccacac accatagctt caaaatgttt ctactccttt tttactcttc cagattttct
      121 cggactccgc gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc
      181 ctctttcttc ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag
      241 agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata aaaattttta tcacgtttct
      301 ttttcttgaa aatttttttt ttgatttttt tctctttcga tgacctccca ttgatattta
      361 agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa
      421 ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa ggactagtaa
      481 aaatgcgcgg agctcctaag aaaaagcgca aagtccggcc ggcatctaga cccggggagc
      541 gccccttcca gtgtcgcatt tgcatgcgga acttttcgcg ccaggacagg cttgacaggc
      601 atacccgtac tcataccggt gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct
      661 cccagaagga gcacttggcg gggcatctac gtacgcacac cggcgagaag ccattccaat
      721 gccgaatatg catgcgcaac ttcagtcgcc gcgacaacct gaaccggcac ctaaaaaccc
      781 acctgaggaa catatgcggc ggaggcacac ctgcagctgc gtcgactcta gaggatccat
      841 ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta
      901 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg
      961 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga
     1021 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga
     1081 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg
     1141 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc
     1201 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg
     1261 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg
     1321 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc
     1381 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga
     1441 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc
     1501 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca
     1561 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg
     1621 agatgctgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg
     1681 agacggacca aagccacttg gccactgcgg gctctacttc atcgcctagg gccgacgcgc
     1741 tggacgattt cgatctcgac atgctgggtt ctgatgccct cgatgacttt gacctggata
     1801 tgttgggaag cgacgcattg gatgactttg atctggacat gctcggctcc gatgctctgg
     1861 acgatttcga tctcgatatg ttaattaact acccgtacga cgttccggac tacgcttctt
     1921 gaggtaccat cggtagaccg gtcttgctag attctaatca agaggatgtc agaatgccat
     1981 ttgcctgaga gatgcaggct tcatttttga tactttttta tttgtaacct atatagtata
     2041 ggattttttt tgtcattttg tttcttctcg tacgagcttg ctcctgatca gcctatctcg
     2101 cagctgatga atatcttgtg gtaggggttt gggaaaatca ttcgagtttg atgtttttct
     2161 tggtatttcc cactcctctt cagagtacag aagattaagt gagagctagc atatcatata
     2221 gaagtcatcg cggcagatca attcatcaat cggagtgagg atattgcgag tttaccacca
     2281 tcaatcggag tgaggatctc caattggtga cggtccagtc atcaatcgga gtgaggattc
     2341 agctgcttct cgaggccgca catcaatcgg agtgaggatg tacagggtgg gctgttccac
     2401 catcaatcgg agtgaggatt tgcgtcaatg gggcggagtt catcaatcgg agtgaggata
     2461 tcgaagtcat cgagagcact catcaatcgg agtgaggata tactccaccc attgacgtca
     2521 catcaatcgg agtgaggatt ggaaccagaa gggtctcttc atcaatcgga gtgaggatac
     2581 cctatgggcg cgccgtcgct ggcaataata gcgggcggac gcatgtcatg agattattgg
     2641 aaaccaccag aatcgaatat aaaaggcgaa cacctttccc aattttggtt tcccctgacc
     2701 caaagacttt aaatttaatt tatttgtccc tatttcaatc aattgaacaa ctatcaaaac
     2761 acagatgaga tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc
     2821 tgctccagtc aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat
     2881 cggttactca gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac
     2941 aaataacggg ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg
     3001 ggtatctctc gagaagagaa tgactccttt gggtccagct tcttccttgc ctcaatcctt
     3061 cttgttgaag tgtttggagc aggttagaaa gatccagggt gatggtgctg ctttgcaaga
     3121 gaagttgtgt gctacttaca agttgtgtca cccagaagag ttggttttgt tgggtcactc
     3181 cttgggtatt ccttgggctc cattgtcctc ttgtccatcc caagctttgc aattggctgg
     3241 ttgtttgtcc caattgcact ccggtttgtt cttgtaccag ggtttgttgc aagctttgga
     3301 gggtatttct ccagagttgg gtccaacttt ggacacattg cagttggacg ttgctgactt
     3361 cgctactact atctggcaac agatggaaga attgggtatg gctccagctt tgcagccaac
     3421 tcaaggtgct atgccagctt ttgcttctgc tttccagaga agagctggtg gtgttttggt
     3481 tgcttctcac ttgcagtctt tcttggaggt ttcctacaga gttttgagac acttggctca
     3541 accataagcg gccgccagct ttctagaaca aaaactcatc tcagaagagg atctgaatag
     3601 cgccgtcgac catcatcatc atcatcattg accggtcttg ctagattcta atcaagagga
     3661 tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta
     3721 acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg
     3781 atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag
     3841 tttgatgttt ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagacc
     3901 ttcgtttgtg cggatccccc acacaccata gcttcaaaat gtttctactc cttttttact
     3961 cttccagatt ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc
     4021 atactaaatt tcccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt
     4081 ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt
     4141 tttatcacgt ttctttttct tgaaaatttt tttttttgat ttttttctct ttcgatgacc
     4201 tcccattgat atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt
     4261 cttgttctat tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa
     4321 tctaagggcg gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     4381 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     4441 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     4501 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     4561 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     4621 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     4681 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     4741 tgcgtgcact tcgtggccga ggagcaggac tgacacgtcc gacgcggccc gacgggtccg
     4801 aggcctcgga gatccgtccc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg
     4861 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     4921 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     4981 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     5041 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc tggagaccaa
     5101 gatctaacat ccaaagacga aaggttgaat gaaacctttt tgccatccga catccacagg
     5161 tccattctca cacataagtg ccaaacgcaa caggagggga tacactagca gcagaccgtt
     5221 gcaaacgcag gacctccact cctcttctcc tcaacaccca cttttgccat cgaaaaacca
     5281 gcccagttat tgggcttgat tggagctcgc tcattccaat tccttctatt aggctactaa
     5341 caccatgact ttattagcct gtctatcctg gcccccctgg cgaggttcat gtttgtttat
     5401 ttccgaatgc aacaagctcc gcattacacc cgaacatcac tccagatgag ggctttctga
     5461 gtgtggggtc aaatagtttc atgttcccca aatggcccaa aactgacagt ttaaacgctg
     5521 tcttggaacc taatatgaca aaagcgtgat ctcatccaag atgaactaag tttggttcgt
     5581 tgaaatgcta acggccagtt ggtcaaaaag aaacttccaa aagtcggcat accgtttgtc
     5641 ttgtttggta ttgattgacg aatgctcaaa aataatctca ttaatgctta gcgcagtctc
     5701 tctatcgctt ctgaaccccg gtgcacctgt gccgaaacgc aaatggggaa acacccgctt
     5761 tttggatgat tatgcattgt ctccacattg tatgcttcca agattctggt gggaatactg
     5821 ctgatagcct aacgttcatg atcaaaattt aactgttcta acccctactt gacagcaata
     5881 tataaacaga aggaagctgc cctgtcttaa accttttttt ttatcatcat tattagctta
     5941 ctttcataat tgcgactggt tccaattgac aagcttttga ttttaacgac ttttaacgac
     6001 aacttgagaa gatcaaaaaa caactaatta ttcgaaacga tgagatttcc ttcaattttt
     6061 actgctgttt tattcgcagc atcctccgca ttagctgctc cagtcaacac tacaacagaa
     6121 gatgaaacgg cacaaattcc ggctgaagct gtcatcggtt actcagattt agaaggggat
     6181 ttcgatgttg ctgttttgcc attttccaac agcacaaata acgggttatt gtttataaat
     6241 actactattg ccagcattgc tgctaaagaa gaaggggtat ctctcgagaa gagattccca
     6301 accattccct tatctagact ttttgacaac gctatgctcc gcgcccatcg tctgcaccag
     6361 ctggcctttg acacctacca ggagtttgaa gaagcctata tcccaaagga acagaagtat
     6421 tcattcctgc agaaccccca gacctccctc tgtttctcag agtctattcc gacaccctcc
     6481 aacagggagg aaacacaaca gaaatccaac ctagagctgc tccgcatctc cctgctgctc
     6541 atccagtcgt ggctggagcc cgtgcagttc ctcaggagtg tcttcgccaa cagcctggtg
     6601 tacggcgcct ctgacagcaa cgtctatgac ctcctaaagg acctagagga aggcatccaa
     6661 acgctgatgg ggaggctgga agatggcagc ccccggactg ggcagatctt caagcagacc
     6721 tacagcaagt tcgacacaaa ctcacacaac gatgacgcac tactcaagaa ctacgggctg
     6781 ctctactgct tcaggaagga catggacaag gtcgagacat tcctgcgcat cgtgcagtgc
     6841 cgctctgtgg agggcagctg tggcttctag accggtcttg ctagattcta atcaagagga
     6901 tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta
     6961 acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg
     7021 atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag
     7081 tttgatgttt ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagaat
     7141 aactgtcgcc tcttttatct gccgcactgc atgaggtgtc cccttagtgg gaaagagtac
     7201 tgagccaacc ctggaggaca gcaagggaaa aatacctaca acttgcttca taatggtcgt
     7261 aaaaacaatc cttgtcggat ataagtgttg tagactgtcc cttatcctct gcgatgttct
     7321 tcctctcaaa gtttgcgatt tctctctatc agaattgcca tcaagagact caggactaat
     7381 ttcgcagtcc cacacgcact cgtacatgat tggctgaaat ttccctaaag aatttctttt
     7441 tcacgaaaat ttttttttac acaagatttt cagcagatat aaaatggaga gcaggacctc
     7501 cgctgtgact cttctttttt ttcttttatt ctcactacat acattttagt tattcgccaa
     7561 cgatgctcga gaaaccagta acattgtatg atgtcgcaga gtatgccggt gtctcttatc
     7621 agactgtttc cagagtggtg aaccaggcca gccatgtttc tgccaaaacc agggaaaaag
     7681 tggaagcagc catggcagag ctgaattaca ttcccaacag agtggcacaa caactggcag
     7741 gcaaacagag cttgctgatt ggagttgcca cctccagtct ggccctgcat gcaccatctc
     7801 aaattgtggc agccattaaa tctagagctg atcaactggg agcctctgtg gtggtgtcaa
     7861 tggtagaaag aagtggagtt gaagcctgta aagctgctgt gcacaatctt ctggcacaaa
     7921 gagtcagtgg gctgatcatt aactatccac tggatgacca ggatgccatt gctgtggaag
     7981 ctgcctgcac taatgttcca gcactctttc ttgatgtctc tgaccagaca cccatcaaca
     8041 gtattatttt ctcccatgaa gatggtacaa gactgggtgt ggagcatctg gttgcattgg
     8101 gacaccagca aattgcactg cttgcgggcc cactcagttc tgtctcagca aggctgagac
     8161 tggctggctg gcataaatat ctcactagga atcaaattca gccaatagct gaaagagaag
     8221 gggactggag tgccatgtct gggtttcaac aaaccatgca aatgctgaat gagggcattg
     8281 ttcccactgc aatgctggtt gccaatgatc agatggcact gggtgcaatg agagccatta
     8341 ctgagtctgg gctgagagtt ggtgcagata tctcggtagt gggatacgac gataccgaag
     8401 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
     8461 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
     8521 tgttgccagt ctcactggtg aagagaaaaa ccaccctggc acccaataca caaactgcct
     8581 ctccccgggc attggctgat tcactcatgc agctagcaag acaggtttcc agactggaaa
     8641 gtgggcagtg atcttgctag attctaatca agaggatgtc agaatgccat ttgcctgaga
     8701 gatgcaggct tcatttttga tactttttta tttgtaacct atatagtata ggattttttt
     8761 tgtcattttg tttcttctcg tacgagcttg ctcctgatca gcctatctcg cagctgatga
     8821 atatcttgtg gtaggggttt gggaaaatca ttcgagtttg atgtttttct tggtatttcc
     8881 cactcctctt cagagtacag aagattaagt gagatcaatt cttgatttag tatacacata
     8941 accaaatttg gatcaagttt gaagtaaaac tttaacttca gctccttaca tttgcactaa
     9001 gatctctgct actctggtcc caagtgaacc accttttgga ccctattgac cggaccttaa
     9061 cttgccaaac ctaaacgctt aatgcctcag acgttttaat gcctctcaac acctccaagg
     9121 ttgctttctt gagcatgcct actagggact ttaacgaact gtggggttgc agacagtttc
     9181 aggcgtgtcc cgaccaatat ggcctactag actctctgaa aaatcacagt tttccagtag
     9241 ttccgatcaa attaccatcg aaatggtccc ataaacggac atttgacatc cgttcctgaa
     9301 ttatagtctt ccaccgtgga tcatggtgtt cctttttttc ccaaagaata tcagcatccc
     9361 ttaactacgt taggtcagtg atgacaatgg accaaattgt tgcaaggttt ttctttttct
     9421 ttcatcggca catttcagcc tcacatgcga ctattatcga tcaatgaaat ccatcaagat
     9481 tgaaatctta aaattgcccc tttcacttga caggatcctt ttttgtagaa atgtcttggt
     9541 gtcctcgtcc aatcaggtag ccatctctga aatatctggc tccgttgcaa ctccgaacga
     9601 cctgctggca acgtaaaatt ctccggggta aaactttaat gtggagtaat ggaaccagaa
     9661 acgtctcttc ccttctctct ccttccaccg cccgttaccg tccctaggaa attttactct
     9721 gctggagagc ttcttctacg gcccccttgc agcaatgctc ttcccagcat tacgttgcgg
     9781 gtaaaacgga ggtcgtgtac ccgacctagc agcccaggga tggaaaagtc ccggccgtcg
     9841 ctggcaataa tagcgggcgg acgcatgtca tgagattatt ggaaaccacc agaatcgaat
     9901 ataaaaggcg aacacctttc ccaattttgg tttctcctga cccaaagact ttaaattgga
     9961 attgtgagcg gataacaatt tcggaattgt gagcggataa caatttaatt tatttgtccc
    10021 tatttcaatc aattgaacaa ctatcaaaac acagaattcg aaacgatgag atttccttca
    10081 atttttactg ctgttttatt cgcagcatcc tccgcattag ctgctccagt caacactaca
    10141 acagaagatg aaacggcaca aattccggct gaagctgtca tcggttactc agatttagaa
    10201 ggggatttcg atgttgctgt tttgccattt tccaacagca caaataacgg gttattgttt
    10261 ataaatacta ctattgccag cattgctgct aaagaagaag gggtatctct cgagaagaga
    10321 tgcgacttgc ctcaaactca ttcactgggt tctcgtagga cattgatgct tcttgcacag
    10381 atgagaagga tctcattatt ttcttgtcta aaagacaggc acgatttcgg attcccacaa
    10441 gaggaatttg gaaatcaatt tcaaaaagct gaaacaattc ctgttttgca tgagatgata
    10501 cagcaaatat ttaacttgtt ttcaaccaaa gattcctcag cagcctggga cgaaaccctt
    10561 ttggacaaat tttacactga actatatcaa cagcttaacg atttggaggc ttgcgtaatt
    10621 caaggagtgg gtgttactga aaccccccta atgaatgaag actctatact ggccgtcaga
    10681 aaatactttc aaagaattac tttgtacttg aaagaaaaaa agtattcacc ttgcgcctgg
    10741 gaggtcgtta gagccgaaat tatgagaagt ttttctttga gtacaaacct gcaggagtca
    10801 ttgcgatcaa aagaatgacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca
    10861 tttgcctgag agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat
    10921 aggatttttt ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc
    10981 gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc
    11041 ttggtatttc ccactcctct tcagagtaca gaagattaag tgagaccttc acatgtgagc
    11101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11701 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11761 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatc
    11821 agatctgagc tcgtttggcc gtggccgtgc tcgtcctcgt cggccggctt gtcgacgacg
    11881 gcggtctccg tcgtcaggat catccgggcc acaagcttgc tgacagaagc ctcaagaaaa
    11941 aaaaaattct tcttcgacta tgctg
//
