LOCUS       sequence-203229-        8824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. When used to produce 
            lentivirus, express physiological levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: p53 (dominant negative R175H mutant)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..516
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     435..453
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    537..561
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             579..1757
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRHCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(1759..1783)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1791..1832
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1857..2445
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1910..1930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2328..2339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2517..2750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2828..2949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2865..2884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2919..2938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2989..3124
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3144..3163)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3145..3163)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3173..3190)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3173..3189)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3182..3204)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3331..3786
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3418..3437)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3628..3649
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3812..3916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3917..3985,3986..4777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4135..4154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5437..5456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5690..5707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5824..5845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5860..5865,5866..5883,5884..5890)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5898..5914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5903..5925
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5922..5938
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5922..5938
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5957..5977
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5959..5977
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6005..6231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6232..6412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6459..6584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7077..7310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7495..7539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7770..7980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7792..7988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7839..7974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8020..8622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8020..8039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8516..8536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8665..8782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
       61 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
      121 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
      181 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
      241 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
      301 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
      361 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
      421 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
      481 tccctcgttg accgaatcac cgacctctct ccccaggggc tagcatcgat ggatcaacaa
      541 gtttgtacaa aaaagttggc tgcagcggcg gcggcaccat ggaggagccg cagtcagatc
      601 ctagcgtcga gccccctctg agtcaggaaa cattttcaga cctatggaaa ctacttcctg
      661 aaaacaacgt tctgtccccc ttgccgtccc aagcaatgga tgatttgatg ctgtccccgg
      721 acgatattga acaatggttc actgaagacc caggtccaga tgaagctccc agaatgccag
      781 aggctgctcc ccgcgtggcc cctgcaccag cagctcctac accggcggcc cctgcaccag
      841 ccccctcctg gcccctgtca tcttctgtcc cttcccagaa aacctaccag ggcagctacg
      901 gtttccgtct gggcttcttg cattctggga cagccaagtc tgtgacttgc acgtactccc
      961 ctgccctcaa caagatgttt tgccaactgg ccaagacctg ccctgtgcag ctgtgggttg
     1021 attccacacc cccgcccggc acccgcgtcc gcgccatggc catctacaag cagtcacagc
     1081 acatgacgga ggttgtgagg cactgccccc accatgagcg ctgctcagat agcgatggtc
     1141 tggcccctcc tcagcatctt atccgagtgg aaggaaattt gcgtgtggag tatttggatg
     1201 acagaaacac ttttcgacat agtgtggtgg tgccctatga gccgcctgag gttggctctg
     1261 actgtaccac catccactac aactacatgt gtaacagttc ctgcatgggc ggcatgaacc
     1321 ggaggcccat cctcaccatc atcacactgg aagactccag tggtaatcta ctgggacgga
     1381 acagctttga ggtgcgtgtt tgtgcctgtc ctgggagaga ccggcgcaca gaggaagaga
     1441 atctccgcaa gaaaggggag cctcaccacg agctgccccc agggagcact aagcgagcac
     1501 tgcccaacaa caccagctcc tctccccagc caaagaagaa accactggat ggagaatatt
     1561 tcacccttca gatccgtggg cgtgagcgct tcgagatgtt ccgagagctg aatgaggcct
     1621 tggaactcaa ggatgcccag gctgggaagg agccgggggg gagcagggct cactccagcc
     1681 acctgaagtc caaaaagggt cagtctacct cccgccataa aaaactcatg ttcaagacag
     1741 aagggcctga ctcagacttg ccaactttct tgtacaaagt ggttaccggt ggtaagccta
     1801 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg accgcgttaa gtcgacaatc
     1861 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     1921 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     1981 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2041 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2101 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2161 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2221 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     2281 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     2341 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     2401 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     2461 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2521 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2581 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2641 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2701 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     2761 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     2821 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2881 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2941 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     3001 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3061 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3121 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     3181 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     3241 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3301 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     3361 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3421 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3481 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3541 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3601 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3661 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3721 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3781 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3841 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3901 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3961 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4021 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4081 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4141 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     4201 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4261 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4321 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4381 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4441 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4501 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4561 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4621 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4681 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4741 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4801 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4861 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5701 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5761 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5821 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5881 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5941 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     6001 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
     6061 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
     6121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
     6181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
     6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     6421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     6481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     6541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     6601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     6661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     6721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     6781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     6841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     6901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     6961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     7021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     7081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     7141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     7201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     7261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     7321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     7381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     7441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     7501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     7561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     7621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     7681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     7741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     7801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     8041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     8641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     8701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     8761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     8821 ccgg
//
