LOCUS       sequence-203239-        9189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MSCV-driven retroviral Foxa1 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, 
            Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, 
            Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ,
            Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y,
            Tuveson DA, Vakoc CR
  TITLE     Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
  JOURNAL   Cell. 2017 Aug 24;170(5):875-888.e20. doi: 
            10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
  PUBMED    28757253
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.007"; journalName: "Cell"; date: 
            "2017-08-24- 24"; volume: "170"; issue: "5"; pages: "875-888.e20"
COMMENT     Sequence Label: MSCV-Neo-GFP/Foxa1
FEATURES             Location/Qualifiers
     source          1..9189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2844..3331
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2879..2902)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3264..3283
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3345..4148
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3408..3427)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4018..4037
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4217..4784
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4234..4786
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4378..4395)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4605..4624
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4798..4800,4801..4803,4804..5517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4843..4864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5104..5123)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5451..5472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5584..6098
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6260..6276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6284..6290,6291..6308,6309..6314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6329..6350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6467..6484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6638..7226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6718..6737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7397..8188,8189..8257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8020..8039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8258..8362)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8430..8448
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8486..8508)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8608..8627
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8821..8843
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8971..8990
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tcaccatgtt agggactgtg aagatggaag
     1441 ggcatgagag caacgactgg aacagctact acgcggacac gcaggaggcc tactcctctg
     1501 tccctgtcag caacatgaac tccggcctgg gctctatgaa ctccatgaac acctacatga
     1561 ccatgaacac catgaccacg agcggcaaca tgaccccggc ttccttcaac atgtcctacg
     1621 ccaacacggg cttaggggcc ggcctgagtc ccggtgctgt ggctggcatg ccaggggcct
     1681 ctgcaggcgc catgaacagc atgactgcgg cgggcgtcac ggccatgggt acggcgctga
     1741 gcccgggagg catgggctcc atgggcgcgc agcccgccac ctccatgaac ggcctgggtc
     1801 cctacgccgc cgccatgaac ccgtgcatga gtcccatggc gtacgcgccg tccaacctgg
     1861 gccgcagccg cgcggggggc ggcggcgacg ccaagacatt caagcgcagc taccctcacg
     1921 ccaagccgcc ttactcctac atctcgctca tcacgatggc catccagcag gcgcccagca
     1981 agatgctcac gctgagcgag atctaccagt ggatcatgga cctcttcccc tattaccgcc
     2041 agaaccagca gcgctggcag aactccatcc gccactcgct gtccttcaac gattgtttcg
     2101 tcaaggtggc acgatccccg gacaagccag gcaagggctc ctactggacg ctgcacccgg
     2161 actccggcaa catgttcgag aacggctgct acttgcgccg ccaaaagcgc ttcaagtgtg
     2221 agaagcagcc gggggccgga ggtgggagtg ggggcggcgg ctccaaaggg ggcccagaaa
     2281 gtcgcaagga cccctcaggc ccggggaacc ccagcgccga gtcacccctt caccggggtg
     2341 tgcacggaaa ggctagccag ctagagggcg cgccggcccc agggcccgcc gccagccccc
     2401 agactctgga ccacagcggg gccacggcga cagggggcgc ttcggagttg aagtctccag
     2461 cgtcttcatc tgcgcccccc ataagctccg ggccaggggc gctagcatct gtacccccct
     2521 ctcacccggc tcacggcctg gcaccccacg aatctcagct gcatctgaaa ggggatcccc
     2581 actactcctt taatcacccc ttctccatca acaacctcat gtcctcctcc gagcaacagc
     2641 acaagctgga cttcaaggca tacgagcagg cgctgcagta ctctccttat ggcgctacct
     2701 tgcccgccag tctgcccctt ggcagcgcct cagtggccac gaggagcccc atcgagccct
     2761 cagccctgga gccagcctac taccaaggtg tgtattccag acccgtgcta aatacttcct
     2821 agctcgagag atctaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     2881 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     2941 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     3001 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     3061 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     3121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     3181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg gggcgggcgc
     3241 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     3301 gttctcctct tcctcatctc cgggcctttc gacctgcagc caatatggga tcggccattg
     3361 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     3421 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     3481 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     3541 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     3601 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     3661 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     3721 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     3781 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     3841 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     3901 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     3961 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     4021 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     4081 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     4141 tcttctgagg ggatcccgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg
     4201 accccatgca tccaattccg cccccccccc ctaacgttac tggccgaagc cgcttggaat
     4261 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     4321 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     4381 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     4441 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4501 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4561 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4621 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4681 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     4741 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     4801 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4861 gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc
     4921 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4981 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5041 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5101 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5161 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5221 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5281 atcaaggcga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5341 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5401 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5461 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagtc
     5521 gacctgcagc caagcttatc gataaaataa aagattttat ttagtctcca gaaaaagggg
     5581 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     5641 gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga acagagagac
     5701 agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     5761 aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat
     5821 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     5881 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     5941 acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc
     6001 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     6061 tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg
     6121 gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact
     6181 gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga
     6241 gctggggaga attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     8401 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     8461 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     8521 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8581 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8641 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     8701 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8761 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8821 cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc
     8881 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     8941 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     9001 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca
     9061 atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca
     9121 gctgaagcct atagagtacg agccatagat aaaataaaag attttattta gtctccagaa
     9181 aaagggggg
//
