LOCUS       sequence-203247-        3544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Omura M, Grosmaitre X, Ma M, Mombaerts P
  TITLE     The beta2-adrenergic receptor as a surrogate odorant receptor in 
            mouse olfactory sensory neurons.
  JOURNAL   Mol Cell Neurosci. 2014 Jan;58:1-10. doi: 10.1016/j.mcn.2013.10.010.
            Epub 2013 Nov 6.
  PUBMED    24211702
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2014-01"; volume: "58:1-10. doi"; pages: " 
            10.1016/j.mcn.2013.10.010. Epub 2013 Nov 6"
COMMENT     Sequence Label: pISH-Olfr1031
FEATURES             Location/Qualifiers
     source          1..3544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(709..727)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(710..727)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(745..761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(745..761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(758..780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    769..785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(793..799,800..817,818..823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    838..859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(976..993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1147..1735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1227..1246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1906..2697,2698..2766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2529..2548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2767..2871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2949..3404)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3086..3107)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3298..3317
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3530..3544,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3544,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tttcctagta ccgtaaatga
      121 gggggttcaa catgggaatg acagtggtat agaacacagc aaccatttta ccttgttcca
      181 cagactctct agatgttggt ctgagataca tgaaaaaaag agttgcatag aatatgatga
      241 cagctgtcag atgggagcca caggtggaga aagctttccg cttgccttct gtagagctaa
      301 tcctcagaat agcaggaaag atatacaggt aggaagtgag aataatgagc agggagaagg
      361 agaggttaaa tccagctaca acaagcatgg aggtttcttt gtgataagtg tcagaacaag
      421 ccagcttaat caatggtgga tcagcacaat agaagtgatt gatttcattg tggccacaga
      481 aagccaggtt gtaagtccac atggtctcca tcagaccagt aagtgcccca tacacataag
      541 gcactgtgat tagagatgtg catacactct gggacatctt gctaccataa agcagagggt
      601 tgcagattgc cacgaaccta tcaaacgcaa tcactagtga attcgcggcc gcctgcaggt
      661 cgaccatatg ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca
      721 cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2881 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2941 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3001 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3061 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     3121 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     3181 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     3241 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     3301 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3361 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     3421 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     3481 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     3541 cgtt
//
