LOCUS       sequence-203248-        4031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to generate a riboprobe for in situ hybridazation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the main olfactory epithelium of
            the mouse.
  JOURNAL   Cell Rep. 2014 Jul 24;8(2):583-95. doi: 
            10.1016/j.celrep.2014.06.010. Epub 2014 Jul 4.
  PUBMED    25001287
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.06.010"; journalName: "Cell Rep"; date: 
            "2014-07-24- 24"; volume: "8"; issue: "2"; pages: "583-95"
COMMENT     Sequence Label: pISH-Taar5
FEATURES             Location/Qualifiers
     source          1..4031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1196..1214)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1197..1214)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1232..1248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1232..1248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(1280..1286,1287..1304,1305..1310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1325..1346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1463..1480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1634..2222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1714..1733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2393..3184,3185..3253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3016..3035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3254..3358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3436..3891)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3573..3594)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3785..3804
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(4017..4031,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4031,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggcccgacgt
       61 cgcatgctcc cggccgccat ggcggccgcg ggaattcgat tatgagagct gtcctcctcc
      121 cgggctctgg agagcagcct acagcgttct gctaccaggt gaatgggtct tgccccagga
      181 cagtccaccc gctggccatc caggtcgtca tctatctggc ctgcgcagtg ggtgtgctga
      241 tcacagtcct ggggaatttg tttgtggtgt ttgcagtgtc ctactttaaa gtgctccaca
      301 cgcccaccaa cttcctgctg ctctccctgg ccctggcaga catgttgcta ggtctgctgg
      361 tgctgcccct gagcacagtc cgctctgtgg agagctgctg gttctttgga gacttcctgt
      421 gccgtctgca tacctatctg gacacgctgt tctgtctcac ctccatcttc catctctgtt
      481 ttatttccat tgaccgtcat tgtgccatct gtgaccccct gctctatccc tccaagttca
      541 cagtcaggac agccctccgc tacatcgtgg cagggtgggg gataccagca gcctacactg
      601 ccttcttcct ctacacagat gtggtagaga gagcactcag ccagcggcta gaagagatgc
      661 cttgtgtggg cagctgccag ctgctgttca ataagttctg gggttggtta aacttccctg
      721 cattttttgt tccctgccta atcatgatca gcttgtacct gaagatcttt gtggtcgcca
      781 ccaggcaggc tcagcggatc agaactctga gccaaagctt ggctggggct gtcaagcggg
      841 aaagaaaagc tgccaagacg ctgggcattg ctgtgggcat ctaccttgtg tgttggcttc
      901 ccttcactgt tgacacacta gtggacagcc ttcttaactt catcacccca cccctggtct
      961 ttgacatctt tatctggttt gcttatttca attcagcctg taatcccatc atctacgtct
     1021 tctcctaccg gtggtttagg aaggcactga aactgctcct gagccgggag atcttctcac
     1081 caaggacacc gactgttgat ttataccatg actgaaatca ctagtgaatt cgcggccgcc
     1141 tgcaggtcga ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat
     1201 agtgtcacct aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1261 atccgatcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3361 catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     3421 aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3481 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3541 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3601 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3661 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3721 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3781 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3841 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc
     3901 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3961 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     4021 gtcacgacgt t
//
