LOCUS       sequence-20326-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..178
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             191..208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             212..244
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(248..256,257..271)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             284..304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttttaactta agcttagcgc agaggcttgg ggcagccgag cggcagccag gccccggccc
       61 gggcctcggt tccagaaggg agaggagccc gccaaggcgc gcaagagagc gggctgcctc
      121 gcagtccgag ccggagaggg agcgcgagcc gcgccggccc cggacggcct ccgaaaccat
      181 ggggggttct catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat
      241 gggtcgggat ctgtacgacg atgacgataa ggtaccagga tcccctaaga agaagaggaa
      301 ggttcggcac cacaagtcgg acccctactc aaccggtcac ctccgcgaag ccccctcccc
      361 gcttatgata cattcccccc agatgtcaca gttccagagc ctgacccacc agtctccacc
      421 ccagcaaaac gtccagccta agaaacagga gctgcgtgct gcctccgtgg tccagcccca
      481 gcccctcgtg gtggtgaagg aggagaagat ccactcaccc atcatccgca gcgagccctt
      541 cagcccctcg ctgcggccgg agccccccaa gcacccggag agcatcaagg cccccgtcca
      601 cctgccccag cggccggaaa tgaagcctgt ggatgtcggg aggcctgtga tccggccccc
      661 agagcagaac gcaccgccac caggggcccc tgacaaggac aaacagaaac aggagccgaa
      721 gactccagtt g
//
