LOCUS       sequence-203280-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In the presence of Cre, drives neuron-specific expression of both 
            GCaMP6s fused to the presynaptic protein synaptophysin and 
            independently the red-fluorescent protein mRuby3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  TITLE     AAV GCaMP6 Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mR...
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(65..86)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(299..355)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(383..397,398..406))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(410..442)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(446..463)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(483..497,498..506))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 ccatgctccc tccggtaccg cccttgtaca gctcgtccat gccgagagtg atcccggcgg
       61 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttcgaaagtt
      121 tggactgcac gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
      181 tgttctgctg gtagtggtag gcgagctgca cgccgccgtc ctcgatgttg tggcggatgt
      241 ggaagttcgc cttgatgccg ttcttctgct tgtcggcctt gatatagacg ttctcgagtg
      301 agctcagccg acctatagct ctgactgcgt gacctgtctt attccactta cgacgtgatg
      361 agtcgaccat ggtggcgaga tccttatcgt catcgtcgta cagatcccga cccatttgct
      421 gtccaccagt catgctagcc ataccatgat gatgatgatg atgagaaccc attggcgaga
      481 tccttatcgt catcgtcgta cagatcccga cccatttgct gtccaccagt cccggtggat
      541 cctgattgga gaaggaggtg ggcgcaccct gttggccata gccttgctgc ccatagtcgc
      601 cctgaggccc gtagccaccg ccaccgctgg ctggctgccc gtaatcgggt tgataaccac
      661 cctgaggccc gtaggagtcc tggggc
//
