LOCUS       sequence-203299-        4156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV-driven retroviral Foxa1 expression (V5-tagged).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, 
            Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, 
            Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ,
            Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y,
            Tuveson DA, Vakoc CR
  TITLE     Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
  JOURNAL   Cell. 2017 Aug 24;170(5):875-888.e20. doi: 
            10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
  PUBMED    28757253
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.007"; journalName: "Cell"; date: 
            "2017-08-24- 24"; volume: "170"; issue: "5"; pages: "875-888.e20"
COMMENT     Sequence Label: MSCV-Neo-GFP/V5-Foxa1
FEATURES             Location/Qualifiers
     source          1..4156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             133..936
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(196..215)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     806..825
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1005..1572
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1022..1574
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1166..1183)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1393..1412
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1586..1588,1589..1591,1592..2305)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1631..1652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1892..1911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2239..2260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2371..2887
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2951..3292
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3359..3775
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3705..3727
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3743..3759
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3789..3830
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
       61 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
      121 cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
      181 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      241 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      301 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
      361 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      421 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      481 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      541 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      601 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      661 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
      721 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      781 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      841 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
      901 atcgccttct atcgccttct tgacgagttc ttctgagggg atcccgcccg ccccacgacc
      961 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc caattccgcc ccccccccct
     1021 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     1081 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     1141 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     1201 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     1261 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     1321 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     1381 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     1441 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     1501 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     1561 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt
     1621 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg
     1681 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1741 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1801 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1861 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1921 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1981 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2041 tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat
     2101 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2161 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2281 cggcatggac gagctgtaca agtaagtcga cctgcagcca agcttatcga taaaataaaa
     2341 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     2401 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     2461 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     2521 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
     2581 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     2641 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     2701 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
     2761 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
     2821 tggactcgct gatccttggg agggtctcct cagattgatt gactgcccac ctcgggggtc
     2881 tttcatttgg aggttccacc gagatttgga gacccctgcc cagggaccac cgaccccccc
     2941 gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg tgcgtgtttg
     3001 tgccggcatc taatgtttgc gcctgcgtct gtactagtta gctaactagc tctgtatctg
     3061 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
     3121 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
     3181 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
     3241 gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg
     3301 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     3361 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     3421 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     3481 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     3541 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     3601 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     3661 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     3721 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat
     3781 tcaccatggg taagcctatc cctaaccctc tcctcggtct cgattctacg ttagggactg
     3841 tgaagatgga agggcatgag agcaacgact ggaacagcta ctacgcggac acgcaggagg
     3901 cctactcctc tgtccctgtc agcaacatga actccggcct gggctctatg aactccatga
     3961 acacctacat gaccatgaac accatgacca cgagcggcaa catgaccccg gcttccttca
     4021 acatgtccta cgccaacacg ggcttagggg ccggcctgag tcccggtgct gtggctggca
     4081 tgccaggggc ctctgcaggc gccatgaaca gcatgactgc ggcgggcgtc acggccatgg
     4141 ggacggcgct gagccc
//
