LOCUS       sequence-203300-       11898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RUNX1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11898)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 11898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-RUNX1
FEATURES             Location/Qualifiers
     source          1..11898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    127..145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    169..187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    211..229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        262..300
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    430..454
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1898..1922)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1975..2001
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        2221..3399
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2452..3390
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3347..3367
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3469..3502
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3509..4126,4127..4132,4133..4168,4169..4171,4172..4207,
                     4208..4210,4211..4246,4247..4255)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4308..4881
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4475..4492)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4702..4721
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4897..4899,4900..4902,4903..5616)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4897..4899,4900..4902,4903..5613)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4942..4963)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5203..5222)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5550..5571
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5752..5771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6059..6647
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6112..6132)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6530..6541)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6722..6955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7074..7092)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7160..7264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7265..7333,7334..8125)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7483..7502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8296..8884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8785..8804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9038..9055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9295..9316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9331..9336,9337..9354,9355..9361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9374..9396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9393..9409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9393..9409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9459..9478
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9670..10303
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10350..10475
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10972..11205
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11390..11434
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11727..11844
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      361 agacaccggg accgatccag cctccgcggc cccgaactag tccagtgtgg tggaattctg
      421 cagatatcaa caagtttgta caaaaaagtt ggcaccatgg cttcagacag catatttgag
      481 tcatttcctt cgtacccaca gtgcttcatg agagaatgca tacttggaat gaatccttct
      541 agagacgtcc acgatgccag cacgagccgc cgcttcacgc cgccttccac cgcgctgagc
      601 ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg acgccggcgc tgccctggcc
      661 ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc tggccgacca cccgggcgag
      721 ctggtgcgca ccgacagccc caacttcctc tgctccgtgc tgcctacgca ctggcgctgc
      781 aacaagaccc tgcccatcgc tttcaaggtg gtggccctag gggatgttcc agatggcact
      841 ctggtcactg tgatggctgg caatgatgaa aactactcgg ctgagctgag aaatgctacc
      901 gcagccatga agaaccaggt tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga
      961 agagggaaaa gcttcactct gaccatcact gtcttcacaa acccaccgca agtcgccacc
     1021 taccacagag ccatcaaaat cacagtggat gggccccgag aacctcgaag acatcggcag
     1081 aaactagatg atcagaccaa gcccgggagc ttgtcctttt ccgagcggct cagtgaactg
     1141 gagcagctgc ggcgcacagc catgagggtc agcccacacc acccagcccc cacgcccaac
     1201 cctcgtgcct ccctgaacca ctccactgcc tttaaccctc agcctcagag tcagatgcag
     1261 gatacaaggc agatccaacc atccccaccg tggtcctacg atcagtccta ccaatacctg
     1321 ggatccattg cctctccttc tgtgcaccca gcaacgccca tttcacctgg acgtgccagc
     1381 ggcatgacaa ccctctctgc agaactttcc agtcgactct caacggcacc cgacctgaca
     1441 gcgttcagcg acccgcgcca gttccccgcg ctgccctcca tctccgaccc ccgcatgcac
     1501 tatccaggcg ccttcaccta ctccccgacg ccggtcacct cgggcatcgg catcggcatg
     1561 tcggccatgg gctcggccac gcgctaccac acctacctgc cgccgcccta ccccggctcg
     1621 tcgcaagcgc agggaggccc gttccaagcc agctcgccct cctaccacct gtactacggc
     1681 gcctcggccg gctcctacca gttctccatg gtgggcggcg agcgctcgcc gccgcgcatc
     1741 ctgccgccct gcaccaacgc ctccaccggc tccgcgctgc tcaaccccag cctcccgaac
     1801 cagagcgacg tggtggaggc cgagggcagc cacagcaact cccccaccaa catggcgccc
     1861 tccgcgcgcc tggaggaggc cgtgtggagg ccctacttgc caactttctt gtacaaagtg
     1921 gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt cgaataccca
     1981 tacgacgtcc cagactacgc ttagtaatga ttaattaaac tagaaattct accgggtagg
     2041 ggaggcgctt ttcccaaggc agtctggagt aacccaccca agatctggcc tccgcgccgg
     2101 gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gccgcgtaag atctggcctc
     2161 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc ctagtcgtga
     2221 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2281 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2341 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2401 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2461 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2521 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2581 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2641 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2701 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2761 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2821 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     2881 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     2941 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3001 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3061 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3121 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3181 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3241 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3301 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3361 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccgcca tgctagtaaa
     3421 ttgtccgcta aattctggcc gtttttggct tttttgttag acggatccat aacttcgtat
     3481 aatgtatgct atacgaagtt atgccaccat gtctagactg gacaagagca aagtcataaa
     3541 cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     3601 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     3661 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     3721 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct
     3781 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     3841 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     3901 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     3961 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     4021 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     4081 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc
     4141 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     4201 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag
     4261 taaggatccg cggccgcact agaggaattc cgcccctctc cccccccccc ctctccctcc
     4321 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     4381 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     4441 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     4501 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     4561 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4621 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4681 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     4741 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4801 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4861 ctttgaaaaa cacgatgata agcttaccgg tccaccatgg tgagcaaggg cgaggagctg
     4921 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4981 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5041 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5101 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5161 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5221 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5281 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5341 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5401 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5461 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5521 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5581 gggatcactc tcggcatgga cgagctgtac aagtaagcat gcccgacggc gaggatctcg
     5641 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5701 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5761 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5821 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5881 gatgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     5941 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6001 gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgcga tagatcctaa
     6061 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6121 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6181 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6241 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6301 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6361 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6421 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6481 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6541 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6601 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6661 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6721 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6961 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7021 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7081 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7141 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7261 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7321 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7381 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7441 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7501 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7561 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7621 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7681 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7741 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7801 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7861 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7921 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7981 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8041 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8101 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8161 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8221 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8941 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9001 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9061 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9121 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9181 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9241 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9421 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9481 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9541 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9601 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9661 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     9721 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     9781 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     9841 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9901 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9961 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    10021 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    10081 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    10141 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10201 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10261 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10321 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10381 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10441 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    10501 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    10561 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    10621 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    10681 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    10741 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    10801 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    10861 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10921 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10981 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11041 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11101 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11161 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11221 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11281 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11341 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11401 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11461 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11521 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11581 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11641 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
    11701 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
    11761 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    11821 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    11881 actaggatcc tttaccac
//
