LOCUS       sequence-20331-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   You J, Croyle JL, Nishimura A, Ozato K, Howley PM
  TITLE     Interaction of the bovine papillomavirus E2 protein with Brd4 
            tethers the viral DNA to host mitotic chromosomes.
  JOURNAL   Cell. 2004 Apr 30. 117(3):349-60.
  PUBMED    15109495
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Apr 30. 117(3):349-60."
COMMENT     Sequence Label: p5071 pcDNA4c hBrd4 (595-1362)
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    5..167
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             180..197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             201..233
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(237..245,246..260)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gcttagcgca gaggcttggg gcagccgagc ggcagccagg ccccggcccg ggcctcggtt
       61 ccagaaggga gaggagcccg ccaaggcgcg caagagagcg ggctgcctcg cagtccgagc
      121 cggagaggga gcgcgagccg cgccggcccc ggacggcctc cgaaaccatg gggggttctc
      181 atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg ggtcgggatc
      241 tgtacgacga tgacgataag gtaccaggat ccccacctcc cacgtatgag tcggaggaag
      301 aggacaagtg caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca
      361 agctccccgg cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc
      421 tgaagaattc caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac
      481 tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg
      541 agaaagttga tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg
      601 agagctccag tgagtccagc tcctctgaca gcgaagactc cgaaacagag atggctccga
      661 agtcaa
//
