LOCUS       sequence-203316-        6951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Chrimson and mCherry in mechanosensory neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Liu M, Sharma AK, Shaevitz JW, Leifer AM
  TITLE     Temporal processing and context dependency in Caenorhabditis elegans
            response to mechanosensation.
  JOURNAL   Elife. 2018 Jun 26;7. pii: 36419. doi: 10.7554/eLife.36419.
  PUBMED    29943731
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-06-26"; volume: "7. pii: 36419. doi"; pages: " 
            10.7554/eLife.36419"
COMMENT     Sequence Label: pmec-4::Chrimson::SL2::mCherry::unc-54
FEATURES             Location/Qualifiers
     source          1..6951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     397..416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(635..739)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(765..1220)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(902..923)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1114..1133
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1347..1369
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1361..1378
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1362..1378
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1388..1407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1388..1406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2470..3519
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE
                     WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI
                     AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY
                     FEWLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV
                     SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK
                     LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE
                     FVEEEDEDTV"
     primer_bind     complement(5525..5545)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5525..5543)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5564..5580)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5564..5580)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5577..5599)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5588..5604
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5612..5618,5619..5636,5637..5642))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5657..5678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5795..5812)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5966..6554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6046..6065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6725..6951,1..565,566..634))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
       61 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
      481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
      781 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
      841 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
      901 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
      961 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     1021 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     1081 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     1141 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     1201 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     1261 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1381 gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggac tagtggatcc
     1441 cccgggctgc agaagcttca atacaagctc aaatacaata ctattttttt tccgtctgaa
     1501 cataaaatta gtttctaaat aaaaaatgtg aaaataaata tttggtttta atcgttaaaa
     1561 tcttccaaat tactaataaa gttcagtcaa ctatatgctt cgaaattcac tgaatctatt
     1621 attgttatta atttttagac ccttggagag ctcccaactc tttcaatcca gaagactgat
     1681 ggatgccagg tgtacttgtc aaaggtggct caaggatgcg agattgtcac ttccaaatcc
     1741 agtgagatga acatctccgt gcaaaccaat gatgatgggg attatgtaag tttttcggat
     1801 tgtagaaccc aatctaaatt tccttttcag tcggagttcc cggttccgga gcagttcaag
     1861 acaacttttg tcaatggcaa gctagtcact gtcgtctcgg acatttgcta aatcttctca
     1921 cgtcataacc ttatttttca acttcatgaa ctatcccctc gaactttttt atttgtccct
     1981 gtctacaatt ctttataagc aattgagaca cactttgctt tattaaataa taccgatttt
     2041 gttttgtata tgcattgact ggtgctcaac tatttttatt gaaaaaatta ctttttactg
     2101 tctcgtcgaa taaaagtgat cccttccaaa aaatagttat tcatgtttga aggtttgtga
     2161 tcgtagaaca attgcaccga atgtagtgta aaaaaaattc cagaatgttc aaatacattt
     2221 ccaccctcac cagaacatct caaacaccag gcacatttca acacactttc atggatctta
     2281 tcttgcctca aaaccatcag ctttcttctc attctacaag tatgaaacaa tctactattc
     2341 tatacattta taatgtaatg gaaccatgtg aagacatgag caagatcatt gtcgtcagcg
     2401 actacccctc cttcattttt cttagagtat cagtttgcta atgctatttt ttttatcgct
     2461 atcaagttaa tggctgagct tatttcatct gcaacacgat cattgttcgc tgcaggagga
     2521 attaatccat ggccaaaccc atatcatcac gaagatatgg gatgcggagg aatgacacca
     2581 actggagagt gtttctctac tgaatggtgg tgcgatccat catatggatt gtctgatgct
     2641 ggatatggat actgttttgt tgaggcaaca ggaggatact tggttgttgg agttgaaaag
     2701 aaacaagcat ggctccattc acgtggaact ccaggagaaa aaattggagc tcaagtttgc
     2761 caatggattg cattctcaat cgctattgca cttttgacat tctatggatt ttctgcttgg
     2821 aaagcaactt gtggatggga agaggtttac gtttgttgcg ttgaggttct tttcgttaca
     2881 ttggagatct tcaaggaatt ttcatctcca gctacagttt atctctcaac tggaaatcat
     2941 gcatattgtc ttcgttactt tgaatggctc ctttcttgcc cagttatcct catcaaactt
     3001 tcaaacttgt ctggactcaa gaacgattac tcaaagagaa caatgggact tattgtttct
     3061 tgcgttggaa tgattgtttt cggaatggct gcaggattgg ctactgattg gctcaagtgg
     3121 ttgctctata ttgtttcatg tatctacgga ggatacatgt actttcaagc tgcaaagtgc
     3181 tatgttgaag caaatcattc tgttccaaaa ggacactgta gaatggttgt taagttgatg
     3241 gcttatgcat acttcgcttc atggggatct tatccaattc tttgggcagt tggaccagaa
     3301 ggacttttga aattgtcacc atacgcaaac tcaatcggac attctatctg tgatatcatc
     3361 gctaaagagt tctggacatt tctcgcacat caccttagaa tcaagatcca tgaacacatc
     3421 cttattcacg gagatattcg aaaaacaact aagatggaga ttggaggaga agaggttgaa
     3481 gttgaagagt ttgttgaaga ggaagatgaa gatactgttg gtaccgctgt ctcatcctac
     3541 tttcacctag ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc
     3601 ctagaagctt acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc
     3661 ttattgaatc tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta
     3721 ttgtattttc aacttctaat tttaattcag ggaaactgct tcaacgcatc atggtctcaa
     3781 agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag gtgcatatgg
     3841 agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc cgaccatatg
     3901 agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta actaaccctg
     3961 attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg gacatcctct
     4021 ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat ataccagatt
     4081 atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat tttgaagacg
     4141 gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg tactaactaa
     4201 ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa gtcaaacttc
     4261 gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg ggatgggaag
     4321 cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt aaacaacgtc
     4381 ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg attttactaa
     4441 ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa aaaccagttc
     4501 agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac aacgaggatt
     4561 acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt ggcatggatg
     4621 aattgtataa ggcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg
     4681 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat
     4741 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     4801 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     4861 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     4921 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     4981 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     5041 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     5101 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     5161 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     5221 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     5281 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     5341 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     5401 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     5461 aaacataact gtttcctact agtcggccgt acgggccctt gggcccggta cccagctttt
     5521 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     5581 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5641 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5701 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5761 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5821 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5881 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5941 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6001 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6061 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6121 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6181 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6241 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6301 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6361 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     6421 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6481 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6541 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6601 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6781 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6841 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6901 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta t
//
